Strain Fitness in Escherichia coli BW25113 around b3229

Experiment: carbon source D-Mannose 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdcuD and sspB are separated by 42 nucleotidessspB and sspA are separated by 5 nucleotidessspA and rpsI are separated by 394 nucleotidesrpsI and rplM are separated by 15 nucleotides b3227: dcuD - predicted transporter (NCBI), at 3,372,891 to 3,374,258 dcuD b3228: sspB - ClpXP protease specificity-enhancing factor (NCBI), at 3,374,301 to 3,374,798 sspB b3229: sspA - stringent starvation protein A (NCBI), at 3,374,804 to 3,375,442 sspA b3230: rpsI - 30S ribosomal protein S9 (NCBI), at 3,375,837 to 3,376,229 rpsI b3231: rplM - 50S ribosomal protein L13 (NCBI), at 3,376,245 to 3,376,673 rplM Position (kb) 3374 3375 3376Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 3373.896 kb on + strand, within dcuDat 3373.897 kb on - strand, within dcuDat 3373.924 kb on + strand, within dcuDat 3373.924 kb on + strand, within dcuDat 3373.932 kb on - strand, within dcuDat 3373.932 kb on - strand, within dcuDat 3373.933 kb on - strandat 3373.933 kb on - strand, within dcuDat 3373.984 kb on + strand, within dcuDat 3374.004 kb on - strand, within dcuDat 3374.052 kb on + strand, within dcuDat 3374.060 kb on - strand, within dcuDat 3374.103 kb on + strand, within dcuDat 3374.128 kb on + strandat 3374.209 kb on - strandat 3374.249 kb on + strandat 3374.249 kb on + strandat 3374.306 kb on - strandat 3374.306 kb on - strandat 3374.366 kb on - strand, within sspBat 3374.472 kb on - strand, within sspBat 3374.472 kb on - strand, within sspBat 3374.484 kb on - strand, within sspBat 3374.484 kb on - strand, within sspBat 3374.514 kb on + strand, within sspBat 3374.543 kb on - strand, within sspBat 3374.543 kb on - strand, within sspBat 3374.563 kb on + strand, within sspBat 3374.667 kb on + strand, within sspBat 3374.667 kb on + strand, within sspBat 3374.675 kb on - strand, within sspBat 3374.675 kb on - strand, within sspBat 3374.684 kb on + strand, within sspBat 3374.684 kb on + strand, within sspBat 3374.966 kb on - strand, within sspAat 3374.976 kb on + strand, within sspAat 3374.976 kb on + strand, within sspAat 3375.071 kb on - strand, within sspAat 3375.073 kb on - strand, within sspAat 3375.084 kb on - strand, within sspAat 3375.098 kb on + strand, within sspAat 3375.098 kb on + strand, within sspAat 3375.113 kb on - strand, within sspAat 3375.189 kb on - strand, within sspAat 3375.220 kb on - strand, within sspAat 3375.220 kb on - strand, within sspAat 3375.220 kb on - strand, within sspAat 3375.236 kb on - strand, within sspAat 3375.353 kb on + strand, within sspAat 3375.353 kb on + strand, within sspAat 3375.515 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 5mM
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3,373,896 + dcuD b3227 0.73 +0.2
3,373,897 - dcuD b3227 0.74 +0.1
3,373,924 + dcuD b3227 0.76 -0.8
3,373,924 + dcuD b3227 0.76 -0.6
3,373,932 - dcuD b3227 0.76 -1.2
3,373,932 - dcuD b3227 0.76 -3.2
3,373,933 - -0.6
3,373,933 - dcuD b3227 0.76 -1.5
3,373,984 + dcuD b3227 0.80 -0.1
3,374,004 - dcuD b3227 0.81 -1.9
3,374,052 + dcuD b3227 0.85 -0.1
3,374,060 - dcuD b3227 0.85 +0.2
3,374,103 + dcuD b3227 0.89 -0.8
3,374,128 + -4.5
3,374,209 - -0.7
3,374,249 + -3.6
3,374,249 + +0.2
3,374,306 - -0.1
3,374,306 - +0.6
3,374,366 - sspB b3228 0.13 +0.3
3,374,472 - sspB b3228 0.34 -0.9
3,374,472 - sspB b3228 0.34 -2.2
3,374,484 - sspB b3228 0.37 -0.6
3,374,484 - sspB b3228 0.37 -2.6
3,374,514 + sspB b3228 0.43 +0.2
3,374,543 - sspB b3228 0.49 +0.0
3,374,543 - sspB b3228 0.49 -1.4
3,374,563 + sspB b3228 0.53 -3.5
3,374,667 + sspB b3228 0.73 +0.2
3,374,667 + sspB b3228 0.73 +1.9
3,374,675 - sspB b3228 0.75 +0.7
3,374,675 - sspB b3228 0.75 -0.2
3,374,684 + sspB b3228 0.77 -1.2
3,374,684 + sspB b3228 0.77 +1.8
3,374,966 - sspA b3229 0.25 +1.1
3,374,976 + sspA b3229 0.27 +2.6
3,374,976 + sspA b3229 0.27 +0.8
3,375,071 - sspA b3229 0.42 +2.5
3,375,073 - sspA b3229 0.42 +0.3
3,375,084 - sspA b3229 0.44 +1.0
3,375,098 + sspA b3229 0.46 +1.6
3,375,098 + sspA b3229 0.46 +1.0
3,375,113 - sspA b3229 0.48 +1.1
3,375,189 - sspA b3229 0.60 +2.4
3,375,220 - sspA b3229 0.65 -1.9
3,375,220 - sspA b3229 0.65 +0.1
3,375,220 - sspA b3229 0.65 +3.5
3,375,236 - sspA b3229 0.68 +4.5
3,375,353 + sspA b3229 0.86 +2.5
3,375,353 + sspA b3229 0.86 +0.1
3,375,515 - +0.7

Or see this region's nucleotide sequence