Strain Fitness in Escherichia coli BW25113 around b2017

Experiment: carbon source D-Mannose 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyeeZ and yoeB are separated by 82 nucleotidesyoeB and yefM overlap by 4 nucleotidesyefM and hisL are separated by 282 nucleotideshisL and hisG are separated by 145 nucleotides b2016: yeeZ - predicted epimerase, with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,086,328 to 2,087,152 yeeZ b4539: yoeB - toxin of the YoeB-YefM toxin-antitoxin system (NCBI), at 2,087,235 to 2,087,489 yoeB b2017: yefM - orf, hypothetical protein (VIMSS), at 2,087,486 to 2,087,737 yefM b2018: hisL - his operon leader peptide (NCBI), at 2,088,020 to 2,088,070 hisL b2019: hisG - ATP phosphoribosyltransferase (NCBI), at 2,088,216 to 2,089,115 hisG Position (kb) 2087 2088Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 2086.500 kb on + strand, within yeeZat 2086.556 kb on - strand, within yeeZat 2086.579 kb on + strand, within yeeZat 2086.660 kb on + strand, within yeeZat 2086.660 kb on + strand, within yeeZat 2086.669 kb on + strand, within yeeZat 2086.672 kb on - strand, within yeeZat 2086.672 kb on - strand, within yeeZat 2086.871 kb on - strand, within yeeZat 2086.947 kb on - strand, within yeeZat 2087.058 kb on + strand, within yeeZat 2087.122 kb on + strandat 2087.178 kb on - strandat 2087.178 kb on - strandat 2087.187 kb on - strandat 2087.197 kb on - strandat 2087.364 kb on - strand, within yoeBat 2087.506 kb on - strandat 2087.506 kb on - strandat 2087.544 kb on + strand, within yefMat 2087.642 kb on - strand, within yefMat 2087.867 kb on + strandat 2088.113 kb on - strandat 2088.203 kb on - strandat 2088.203 kb on - strandat 2088.316 kb on + strand, within hisGat 2088.484 kb on - strand, within hisGat 2088.602 kb on + strand, within hisGat 2088.610 kb on - strand, within hisGat 2088.610 kb on - strand, within hisGat 2088.657 kb on - strand, within hisGat 2088.681 kb on - strand, within hisG

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 5mM
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2,086,500 + yeeZ b2016 0.21 -1.5
2,086,556 - yeeZ b2016 0.28 +0.5
2,086,579 + yeeZ b2016 0.30 +0.2
2,086,660 + yeeZ b2016 0.40 +1.6
2,086,660 + yeeZ b2016 0.40 +0.2
2,086,669 + yeeZ b2016 0.41 -0.8
2,086,672 - yeeZ b2016 0.42 -0.9
2,086,672 - yeeZ b2016 0.42 +1.9
2,086,871 - yeeZ b2016 0.66 +0.2
2,086,947 - yeeZ b2016 0.75 -1.0
2,087,058 + yeeZ b2016 0.88 +0.5
2,087,122 + +0.3
2,087,178 - -1.9
2,087,178 - -0.3
2,087,187 - +0.7
2,087,197 - -0.6
2,087,364 - yoeB b4539 0.51 +0.6
2,087,506 - +0.4
2,087,506 - +0.6
2,087,544 + yefM b2017 0.23 -3.0
2,087,642 - yefM b2017 0.62 +2.2
2,087,867 + +0.3
2,088,113 - +0.9
2,088,203 - +0.3
2,088,203 - -1.8
2,088,316 + hisG b2019 0.11 -4.5
2,088,484 - hisG b2019 0.30 -5.4
2,088,602 + hisG b2019 0.43 -3.9
2,088,610 - hisG b2019 0.44 -5.6
2,088,610 - hisG b2019 0.44 -5.8
2,088,657 - hisG b2019 0.49 -3.3
2,088,681 - hisG b2019 0.52 -4.0

Or see this region's nucleotide sequence