Strain Fitness in Escherichia coli BW25113 around b0429

Experiment: carbon source D-Mannose 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcyoE and cyoD are separated by 11 nucleotidescyoD and cyoC overlap by 1 nucleotidescyoC and cyoB overlap by 11 nucleotides b0428: cyoE - protoheme IX farnesyltransferase (NCBI), at 446,039 to 446,929 cyoE b0429: cyoD - cytochrome o ubiquinol oxidase subunit IV (NCBI), at 446,941 to 447,270 cyoD b0430: cyoC - cytochrome o ubiquinol oxidase subunit III (NCBI), at 447,270 to 447,884 cyoC b0431: cyoB - cytochrome o ubiquinol oxidase subunit I (NCBI), at 447,874 to 449,865 cyoB Position (kb) 446 447 448Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 445.978 kb on + strandat 446.028 kb on - strandat 446.028 kb on - strandat 446.054 kb on + strandat 446.069 kb on - strandat 446.069 kb on - strandat 446.096 kb on + strandat 446.101 kb on - strandat 446.119 kb on + strandat 446.140 kb on - strand, within cyoEat 446.161 kb on - strand, within cyoEat 446.242 kb on - strand, within cyoEat 446.297 kb on - strand, within cyoEat 446.394 kb on + strand, within cyoEat 446.394 kb on + strand, within cyoEat 446.394 kb on + strand, within cyoEat 446.407 kb on - strand, within cyoEat 446.525 kb on + strand, within cyoEat 446.525 kb on + strand, within cyoEat 446.533 kb on - strand, within cyoEat 446.562 kb on - strand, within cyoEat 446.562 kb on - strand, within cyoEat 446.657 kb on + strand, within cyoEat 446.657 kb on + strand, within cyoEat 446.673 kb on - strand, within cyoEat 446.677 kb on + strand, within cyoEat 446.677 kb on + strand, within cyoEat 446.796 kb on - strand, within cyoEat 446.824 kb on + strand, within cyoEat 446.832 kb on - strand, within cyoEat 446.880 kb on + strandat 446.880 kb on + strandat 446.932 kb on + strandat 446.944 kb on + strandat 446.981 kb on - strand, within cyoDat 447.018 kb on - strand, within cyoDat 447.093 kb on - strand, within cyoDat 447.093 kb on - strand, within cyoDat 447.105 kb on - strand, within cyoDat 447.161 kb on - strand, within cyoDat 447.167 kb on + strand, within cyoDat 447.175 kb on - strand, within cyoDat 447.201 kb on + strand, within cyoDat 447.223 kb on - strand, within cyoDat 447.285 kb on - strandat 447.303 kb on - strandat 447.347 kb on + strand, within cyoCat 447.351 kb on + strand, within cyoCat 447.373 kb on - strand, within cyoCat 447.407 kb on + strand, within cyoCat 447.418 kb on - strand, within cyoCat 447.445 kb on - strand, within cyoCat 447.492 kb on + strand, within cyoCat 447.492 kb on + strand, within cyoCat 447.521 kb on + strand, within cyoCat 447.570 kb on + strand, within cyoCat 447.580 kb on + strand, within cyoCat 447.679 kb on + strand, within cyoCat 447.956 kb on + strandat 447.964 kb on - strandat 447.964 kb on - strandat 447.989 kb on + strandat 448.002 kb on - strandat 448.005 kb on + strandat 448.092 kb on - strand, within cyoBat 448.092 kb on - strand, within cyoBat 448.134 kb on - strand, within cyoBat 448.134 kb on - strand, within cyoBat 448.238 kb on - strand, within cyoB

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 5mM
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445,978 + -0.9
446,028 - +0.4
446,028 - -0.7
446,054 + -0.2
446,069 - -0.7
446,069 - -0.1
446,096 + +0.4
446,101 - +1.1
446,119 + -0.1
446,140 - cyoE b0428 0.11 +1.1
446,161 - cyoE b0428 0.14 +0.5
446,242 - cyoE b0428 0.23 +0.1
446,297 - cyoE b0428 0.29 +0.8
446,394 + cyoE b0428 0.40 +0.3
446,394 + cyoE b0428 0.40 +0.5
446,394 + cyoE b0428 0.40 -1.7
446,407 - cyoE b0428 0.41 -0.5
446,525 + cyoE b0428 0.55 +0.7
446,525 + cyoE b0428 0.55 -1.5
446,533 - cyoE b0428 0.55 +1.4
446,562 - cyoE b0428 0.59 -0.8
446,562 - cyoE b0428 0.59 -0.2
446,657 + cyoE b0428 0.69 +0.6
446,657 + cyoE b0428 0.69 -0.4
446,673 - cyoE b0428 0.71 -0.2
446,677 + cyoE b0428 0.72 +0.6
446,677 + cyoE b0428 0.72 -0.8
446,796 - cyoE b0428 0.85 +1.2
446,824 + cyoE b0428 0.88 +0.5
446,832 - cyoE b0428 0.89 +1.0
446,880 + -0.9
446,880 + +1.5
446,932 + -3.7
446,944 + -1.2
446,981 - cyoD b0429 0.12 +0.2
447,018 - cyoD b0429 0.23 +0.7
447,093 - cyoD b0429 0.46 +2.5
447,093 - cyoD b0429 0.46 +1.1
447,105 - cyoD b0429 0.50 -0.9
447,161 - cyoD b0429 0.67 +1.7
447,167 + cyoD b0429 0.68 +0.3
447,175 - cyoD b0429 0.71 -3.7
447,201 + cyoD b0429 0.79 +2.2
447,223 - cyoD b0429 0.85 +0.5
447,285 - +1.2
447,303 - +0.5
447,347 + cyoC b0430 0.13 +1.4
447,351 + cyoC b0430 0.13 +0.4
447,373 - cyoC b0430 0.17 +0.0
447,407 + cyoC b0430 0.22 +0.2
447,418 - cyoC b0430 0.24 +0.1
447,445 - cyoC b0430 0.28 +0.6
447,492 + cyoC b0430 0.36 +1.2
447,492 + cyoC b0430 0.36 +2.6
447,521 + cyoC b0430 0.41 +2.2
447,570 + cyoC b0430 0.49 +1.4
447,580 + cyoC b0430 0.50 -0.5
447,679 + cyoC b0430 0.67 -0.2
447,956 + +0.8
447,964 - -1.0
447,964 - -2.6
447,989 + +0.5
448,002 - +0.2
448,005 + -0.9
448,092 - cyoB b0431 0.11 +1.3
448,092 - cyoB b0431 0.11 +3.4
448,134 - cyoB b0431 0.13 -0.9
448,134 - cyoB b0431 0.13 -3.7
448,238 - cyoB b0431 0.18 +0.3

Or see this region's nucleotide sequence