Experiment: carbon source D-Mannose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykgK and ykgL are separated by 775 nucleotides ykgL and ykgO are separated by 34 nucleotides ykgO and ykgM overlap by 1 nucleotides ykgM and ykgR are separated by 363 nucleotides
b0294: ykgK - predicted regulator (NCBI), at 309,970 to 310,560
ykgK
b0295: ykgL - hypothetical protein (NCBI), at 311,336 to 311,563
ykgL
b4506: ykgO - rpmJ (L36) paralog (NCBI), at 311,598 to 311,738
ykgO
b0296: ykgM - 50S ribosomal protein L31 (NCBI), at 311,738 to 312,001
ykgM
b4671: ykgR - expressed protein (RefSeq), at 312,365 to 312,466
ykgR
Position (kb) 311
312 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 310.434 kb on - strand, within ykgK at 310.459 kb on - strand, within ykgK at 310.646 kb on - strand at 310.829 kb on + strand at 310.880 kb on - strand at 310.891 kb on - strand at 310.891 kb on - strand at 310.892 kb on - strand at 310.892 kb on - strand at 310.894 kb on - strand at 310.904 kb on - strand at 311.087 kb on - strand at 311.091 kb on - strand at 311.100 kb on + strand at 311.100 kb on + strand at 311.114 kb on - strand at 311.138 kb on - strand at 311.172 kb on - strand at 311.172 kb on - strand at 311.194 kb on - strand at 311.222 kb on - strand at 311.222 kb on - strand at 311.256 kb on + strand at 311.260 kb on + strand at 311.260 kb on + strand at 311.352 kb on + strand at 311.360 kb on + strand, within ykgL at 311.360 kb on + strand, within ykgL at 311.364 kb on - strand, within ykgL at 311.370 kb on + strand, within ykgL at 311.380 kb on + strand, within ykgL at 311.385 kb on + strand, within ykgL at 311.385 kb on + strand, within ykgL at 311.396 kb on + strand, within ykgL at 311.396 kb on + strand, within ykgL at 311.402 kb on + strand, within ykgL at 311.402 kb on + strand, within ykgL at 311.418 kb on - strand, within ykgL at 311.470 kb on + strand, within ykgL at 311.470 kb on + strand, within ykgL at 311.525 kb on + strand, within ykgL at 311.626 kb on + strand, within ykgO at 311.626 kb on + strand, within ykgO at 311.634 kb on - strand, within ykgO at 311.634 kb on - strand, within ykgO at 311.634 kb on - strand, within ykgO at 311.722 kb on + strand, within ykgO at 311.722 kb on + strand, within ykgO at 311.829 kb on + strand, within ykgM at 311.829 kb on - strand, within ykgM at 311.944 kb on - strand, within ykgM at 311.977 kb on + strand at 311.985 kb on - strand at 312.124 kb on + strand at 312.124 kb on + strand at 312.125 kb on + strand at 312.144 kb on + strand at 312.146 kb on + strand at 312.146 kb on + strand at 312.215 kb on + strand at 312.216 kb on + strand at 312.258 kb on + strand at 312.258 kb on + strand at 312.260 kb on + strand at 312.269 kb on + strand at 312.488 kb on - strand at 312.488 kb on - strand at 312.488 kb on - strand at 312.488 kb on - strand at 312.490 kb on - strand at 312.505 kb on - strand at 312.520 kb on - strand at 312.522 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Mannose 5mM remove 310,434 - ykgK b0294 0.79 +2.4 310,459 - ykgK b0294 0.83 +2.4 310,646 - +5.2 310,829 + +2.4 310,880 - +2.4 310,891 - +2.4 310,891 - -0.6 310,892 - +0.4 310,892 - -0.5 310,894 - +0.4 310,904 - -1.0 311,087 - +1.0 311,091 - +0.4 311,100 + -1.7 311,100 + -1.0 311,114 - -1.7 311,138 - -2.6 311,172 - +1.5 311,172 - +1.2 311,194 - +2.3 311,222 - -0.8 311,222 - -3.0 311,256 + -0.6 311,260 + +1.8 311,260 + +0.2 311,352 + +0.3 311,360 + ykgL b0295 0.11 +2.1 311,360 + ykgL b0295 0.11 +0.1 311,364 - ykgL b0295 0.12 +0.7 311,370 + ykgL b0295 0.15 +0.4 311,380 + ykgL b0295 0.19 -1.0 311,385 + ykgL b0295 0.21 +1.0 311,385 + ykgL b0295 0.21 +1.1 311,396 + ykgL b0295 0.26 +0.4 311,396 + ykgL b0295 0.26 +2.0 311,402 + ykgL b0295 0.29 +0.5 311,402 + ykgL b0295 0.29 +0.8 311,418 - ykgL b0295 0.36 +1.4 311,470 + ykgL b0295 0.59 +1.6 311,470 + ykgL b0295 0.59 +1.4 311,525 + ykgL b0295 0.83 +2.4 311,626 + ykgO b4506 0.20 +1.4 311,626 + ykgO b4506 0.20 +1.5 311,634 - ykgO b4506 0.26 +0.2 311,634 - ykgO b4506 0.26 -0.4 311,634 - ykgO b4506 0.26 -0.1 311,722 + ykgO b4506 0.88 -1.1 311,722 + ykgO b4506 0.88 -1.1 311,829 + ykgM b0296 0.34 -0.7 311,829 - ykgM b0296 0.34 +0.6 311,944 - ykgM b0296 0.78 +1.7 311,977 + -1.8 311,985 - +0.3 312,124 + +0.3 312,124 + -1.0 312,125 + -0.2 312,144 + -0.8 312,146 + -0.7 312,146 + -1.5 312,215 + -0.3 312,216 + +0.4 312,258 + +0.9 312,258 + -2.5 312,260 + +0.5 312,269 + +0.8 312,488 - -0.2 312,488 - -2.2 312,488 - +0.4 312,488 - -0.8 312,490 - -2.3 312,505 - -0.6 312,520 - -0.1 312,522 - +0.4
Or see this region's nucleotide sequence