Experiment: carbon source D-Glucose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdtQ and yohP are separated by 223 nucleotides yohP and dusC are separated by 372 nucleotides dusC and yohJ are separated by 238 nucleotides yohJ and yohK overlap by 4 nucleotides
b2139: mdtQ - orf, hypothetical protein (VIMSS), at 2,225,345 to 2,226,780
mdtQ
b4679: yohP - expressed protein (RefSeq), at 2,227,004 to 2,227,087
yohP
b2140: dusC - tRNA-dihydrouridine synthase C (NCBI), at 2,227,460 to 2,228,407
dusC
b2141: yohJ - hypothetical protein (NCBI), at 2,228,646 to 2,229,044
yohJ
b2142: yohK - predicted inner membrane protein (NCBI), at 2,229,041 to 2,229,736
yohK
Position (kb)
2227
2228
2229 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2226.528 kb on + strand, within mdtQ at 2226.528 kb on + strand, within mdtQ at 2226.668 kb on - strand at 2226.721 kb on + strand at 2226.738 kb on + strand at 2226.753 kb on + strand at 2226.753 kb on + strand at 2226.782 kb on + strand at 2226.782 kb on + strand at 2226.794 kb on + strand at 2226.903 kb on + strand at 2226.934 kb on + strand at 2226.934 kb on + strand at 2226.942 kb on - strand at 2227.010 kb on + strand at 2227.020 kb on + strand, within yohP at 2227.020 kb on + strand, within yohP at 2227.047 kb on + strand, within yohP at 2227.047 kb on + strand, within yohP at 2227.050 kb on + strand, within yohP at 2227.051 kb on + strand, within yohP at 2227.052 kb on + strand, within yohP at 2227.052 kb on + strand, within yohP at 2227.052 kb on + strand, within yohP at 2227.126 kb on + strand at 2227.158 kb on - strand at 2227.158 kb on - strand at 2227.163 kb on - strand at 2227.167 kb on + strand at 2227.168 kb on - strand at 2227.175 kb on - strand at 2227.179 kb on - strand at 2227.179 kb on - strand at 2227.336 kb on + strand at 2227.336 kb on + strand at 2227.345 kb on + strand at 2227.355 kb on + strand at 2227.432 kb on + strand at 2227.592 kb on - strand, within dusC at 2227.617 kb on - strand, within dusC at 2227.620 kb on - strand, within dusC at 2227.620 kb on - strand, within dusC at 2227.663 kb on + strand, within dusC at 2227.663 kb on + strand, within dusC at 2227.671 kb on - strand, within dusC at 2227.671 kb on - strand, within dusC at 2227.671 kb on - strand, within dusC at 2227.671 kb on - strand, within dusC at 2227.671 kb on - strand, within dusC at 2227.674 kb on + strand, within dusC at 2227.675 kb on - strand, within dusC at 2227.676 kb on - strand, within dusC at 2227.747 kb on - strand, within dusC at 2227.752 kb on - strand, within dusC at 2227.752 kb on - strand, within dusC at 2227.767 kb on + strand, within dusC at 2227.767 kb on + strand, within dusC at 2227.791 kb on - strand, within dusC at 2227.791 kb on - strand, within dusC at 2227.791 kb on - strand, within dusC at 2227.808 kb on - strand, within dusC at 2227.808 kb on - strand, within dusC at 2227.862 kb on + strand, within dusC at 2227.862 kb on + strand, within dusC at 2227.891 kb on - strand, within dusC at 2227.918 kb on - strand, within dusC at 2228.138 kb on + strand, within dusC at 2228.327 kb on - strand at 2228.444 kb on - strand at 2228.444 kb on - strand at 2228.446 kb on - strand at 2228.446 kb on - strand at 2228.530 kb on - strand at 2228.542 kb on - strand at 2228.542 kb on - strand at 2228.637 kb on - strand at 2228.637 kb on - strand at 2228.716 kb on - strand, within yohJ at 2228.716 kb on - strand, within yohJ at 2228.753 kb on + strand, within yohJ at 2228.753 kb on + strand, within yohJ at 2228.951 kb on + strand, within yohJ at 2229.015 kb on + strand at 2229.068 kb on - strand at 2229.123 kb on - strand, within yohK at 2229.123 kb on - strand, within yohK at 2229.123 kb on - strand, within yohK at 2229.123 kb on - strand, within yohK at 2229.190 kb on + strand, within yohK at 2229.221 kb on + strand, within yohK at 2229.221 kb on + strand, within yohK at 2229.259 kb on + strand, within yohK at 2229.272 kb on - strand, within yohK at 2229.279 kb on + strand, within yohK at 2229.279 kb on + strand, within yohK at 2229.284 kb on + strand, within yohK at 2229.284 kb on + strand, within yohK at 2229.292 kb on - strand, within yohK
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 5mM remove 2,226,528 + mdtQ b2139 0.82 -2.6 2,226,528 + mdtQ b2139 0.82 +1.0 2,226,668 - +0.2 2,226,721 + +0.8 2,226,738 + -0.3 2,226,753 + +0.4 2,226,753 + -0.1 2,226,782 + -3.1 2,226,782 + -2.5 2,226,794 + +0.1 2,226,903 + +1.3 2,226,934 + -2.6 2,226,934 + -0.5 2,226,942 - +2.8 2,227,010 + +1.2 2,227,020 + yohP b4679 0.19 -0.9 2,227,020 + yohP b4679 0.19 -2.0 2,227,047 + yohP b4679 0.51 -0.6 2,227,047 + yohP b4679 0.51 -2.2 2,227,050 + yohP b4679 0.55 +0.0 2,227,051 + yohP b4679 0.56 +0.8 2,227,052 + yohP b4679 0.57 -1.7 2,227,052 + yohP b4679 0.57 -0.2 2,227,052 + yohP b4679 0.57 -2.8 2,227,126 + +2.8 2,227,158 - +2.8 2,227,158 - +1.8 2,227,163 - -3.4 2,227,167 + -2.4 2,227,168 - -0.1 2,227,175 - -0.1 2,227,179 - +2.8 2,227,179 - +2.8 2,227,336 + -2.9 2,227,336 + -1.7 2,227,345 + -1.3 2,227,355 + +0.8 2,227,432 + +2.8 2,227,592 - dusC b2140 0.14 -0.7 2,227,617 - dusC b2140 0.17 -0.9 2,227,620 - dusC b2140 0.17 +1.6 2,227,620 - dusC b2140 0.17 +0.4 2,227,663 + dusC b2140 0.21 +2.8 2,227,663 + dusC b2140 0.21 +2.8 2,227,671 - dusC b2140 0.22 +0.3 2,227,671 - dusC b2140 0.22 +0.1 2,227,671 - dusC b2140 0.22 +0.8 2,227,671 - dusC b2140 0.22 +0.7 2,227,671 - dusC b2140 0.22 +1.4 2,227,674 + dusC b2140 0.23 +0.2 2,227,675 - dusC b2140 0.23 -0.0 2,227,676 - dusC b2140 0.23 -2.1 2,227,747 - dusC b2140 0.30 -0.6 2,227,752 - dusC b2140 0.31 -0.9 2,227,752 - dusC b2140 0.31 -2.3 2,227,767 + dusC b2140 0.32 +4.2 2,227,767 + dusC b2140 0.32 -2.2 2,227,791 - dusC b2140 0.35 +0.7 2,227,791 - dusC b2140 0.35 +1.5 2,227,791 - dusC b2140 0.35 +0.9 2,227,808 - dusC b2140 0.37 -1.4 2,227,808 - dusC b2140 0.37 -2.9 2,227,862 + dusC b2140 0.42 -2.4 2,227,862 + dusC b2140 0.42 +0.0 2,227,891 - dusC b2140 0.45 +1.0 2,227,918 - dusC b2140 0.48 -1.3 2,228,138 + dusC b2140 0.72 +0.5 2,228,327 - -3.2 2,228,444 - -1.6 2,228,444 - -2.6 2,228,446 - -0.4 2,228,446 - -3.8 2,228,530 - +0.9 2,228,542 - +1.8 2,228,542 - +0.9 2,228,637 - -2.6 2,228,637 - -0.0 2,228,716 - yohJ b2141 0.18 +2.6 2,228,716 - yohJ b2141 0.18 +0.8 2,228,753 + yohJ b2141 0.27 +0.2 2,228,753 + yohJ b2141 0.27 -1.6 2,228,951 + yohJ b2141 0.76 +0.7 2,229,015 + +2.8 2,229,068 - +0.4 2,229,123 - yohK b2142 0.12 +3.2 2,229,123 - yohK b2142 0.12 +2.1 2,229,123 - yohK b2142 0.12 +0.6 2,229,123 - yohK b2142 0.12 +2.2 2,229,190 + yohK b2142 0.21 +0.5 2,229,221 + yohK b2142 0.26 +2.0 2,229,221 + yohK b2142 0.26 +0.4 2,229,259 + yohK b2142 0.31 +1.3 2,229,272 - yohK b2142 0.33 -4.0 2,229,279 + yohK b2142 0.34 -3.5 2,229,279 + yohK b2142 0.34 -0.9 2,229,284 + yohK b2142 0.35 -0.2 2,229,284 + yohK b2142 0.35 -2.1 2,229,292 - yohK b2142 0.36 +1.7
Or see this region's nucleotide sequence