Strain Fitness in Escherichia coli BW25113 around b2012

Experiment: carbon source D-Glucose 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsbcB and yeeD are separated by 42 nucleotidesyeeD and yeeE are separated by 13 nucleotides b2011: sbcB - exonuclease I (NCBI), at 2,080,780 to 2,082,207 sbcB b2012: yeeD - hypothetical protein (NCBI), at 2,082,250 to 2,082,477 yeeD b2013: yeeE - predicted inner membrane protein (NCBI), at 2,082,491 to 2,083,549 yeeE Position (kb) 2082 2083Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2081.289 kb on - strand, within sbcBat 2081.289 kb on - strand, within sbcBat 2081.300 kb on + strand, within sbcBat 2081.307 kb on + strand, within sbcBat 2081.308 kb on - strand, within sbcBat 2081.308 kb on - strand, within sbcBat 2081.410 kb on - strand, within sbcBat 2081.411 kb on + strand, within sbcBat 2081.411 kb on + strand, within sbcBat 2081.513 kb on + strand, within sbcBat 2081.518 kb on - strand, within sbcBat 2081.625 kb on + strand, within sbcBat 2081.675 kb on + strand, within sbcBat 2081.679 kb on + strand, within sbcBat 2081.688 kb on - strand, within sbcBat 2081.701 kb on + strand, within sbcBat 2081.862 kb on - strand, within sbcBat 2081.862 kb on - strand, within sbcBat 2081.885 kb on - strand, within sbcBat 2082.093 kb on + strandat 2082.093 kb on + strandat 2082.168 kb on + strandat 2082.266 kb on + strandat 2082.266 kb on + strandat 2082.266 kb on + strandat 2082.299 kb on + strand, within yeeDat 2082.299 kb on + strand, within yeeDat 2082.429 kb on - strand, within yeeDat 2082.537 kb on - strandat 2082.537 kb on - strandat 2082.544 kb on - strandat 2082.544 kb on - strandat 2082.546 kb on + strandat 2082.674 kb on + strand, within yeeEat 2082.694 kb on - strand, within yeeEat 2082.775 kb on - strand, within yeeEat 2082.775 kb on - strand, within yeeEat 2082.851 kb on - strand, within yeeEat 2082.881 kb on + strand, within yeeEat 2082.897 kb on - strand, within yeeEat 2082.897 kb on - strand, within yeeEat 2082.915 kb on - strand, within yeeEat 2082.973 kb on + strand, within yeeEat 2082.973 kb on + strand, within yeeEat 2082.981 kb on - strand, within yeeEat 2083.040 kb on - strand, within yeeEat 2083.040 kb on - strand, within yeeEat 2083.126 kb on + strand, within yeeEat 2083.139 kb on + strand, within yeeEat 2083.183 kb on + strand, within yeeEat 2083.186 kb on - strand, within yeeEat 2083.186 kb on - strand, within yeeEat 2083.258 kb on - strand, within yeeEat 2083.310 kb on + strand, within yeeEat 2083.319 kb on - strand, within yeeEat 2083.319 kb on - strand, within yeeEat 2083.462 kb on + strandat 2083.470 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 5mM
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2,081,289 - sbcB b2011 0.36 -0.0
2,081,289 - sbcB b2011 0.36 +0.6
2,081,300 + sbcB b2011 0.36 +1.2
2,081,307 + sbcB b2011 0.37 -1.2
2,081,308 - sbcB b2011 0.37 -0.2
2,081,308 - sbcB b2011 0.37 -2.1
2,081,410 - sbcB b2011 0.44 +0.4
2,081,411 + sbcB b2011 0.44 -0.8
2,081,411 + sbcB b2011 0.44 +0.1
2,081,513 + sbcB b2011 0.51 +1.0
2,081,518 - sbcB b2011 0.52 -3.2
2,081,625 + sbcB b2011 0.59 +3.1
2,081,675 + sbcB b2011 0.63 +0.3
2,081,679 + sbcB b2011 0.63 -1.0
2,081,688 - sbcB b2011 0.64 -0.5
2,081,701 + sbcB b2011 0.64 -0.1
2,081,862 - sbcB b2011 0.76 -2.4
2,081,862 - sbcB b2011 0.76 -3.3
2,081,885 - sbcB b2011 0.77 +1.6
2,082,093 + +2.0
2,082,093 + +0.5
2,082,168 + -2.5
2,082,266 + -0.3
2,082,266 + +1.3
2,082,266 + +1.7
2,082,299 + yeeD b2012 0.21 -2.4
2,082,299 + yeeD b2012 0.21 +2.6
2,082,429 - yeeD b2012 0.79 +0.1
2,082,537 - -2.9
2,082,537 - +0.2
2,082,544 - +0.4
2,082,544 - -3.0
2,082,546 + -1.5
2,082,674 + yeeE b2013 0.17 -4.4
2,082,694 - yeeE b2013 0.19 -1.0
2,082,775 - yeeE b2013 0.27 -1.5
2,082,775 - yeeE b2013 0.27 -0.8
2,082,851 - yeeE b2013 0.34 +1.1
2,082,881 + yeeE b2013 0.37 -0.3
2,082,897 - yeeE b2013 0.38 -3.2
2,082,897 - yeeE b2013 0.38 -0.3
2,082,915 - yeeE b2013 0.40 -0.7
2,082,973 + yeeE b2013 0.46 -1.4
2,082,973 + yeeE b2013 0.46 -2.4
2,082,981 - yeeE b2013 0.46 +1.2
2,083,040 - yeeE b2013 0.52 +0.6
2,083,040 - yeeE b2013 0.52 -3.3
2,083,126 + yeeE b2013 0.60 +0.4
2,083,139 + yeeE b2013 0.61 +0.7
2,083,183 + yeeE b2013 0.65 +0.7
2,083,186 - yeeE b2013 0.66 -0.4
2,083,186 - yeeE b2013 0.66 -2.5
2,083,258 - yeeE b2013 0.72 +0.9
2,083,310 + yeeE b2013 0.77 -0.6
2,083,319 - yeeE b2013 0.78 +1.1
2,083,319 - yeeE b2013 0.78 +1.5
2,083,462 + -0.3
2,083,470 + -3.0

Or see this region's nucleotide sequence