Experiment: carbon source D-Glucose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydgG and pntB are separated by 24 nucleotides pntB and pntA are separated by 10 nucleotides
b1601: ydgG - predicted inner membrane protein (NCBI), at 1,671,937 to 1,672,971
ydgG
b1602: pntB - pyridine nucleotide transhydrogenase (NCBI), at 1,672,996 to 1,674,384
pntB
b1603: pntA - NAD(P) transhydrogenase subunit alpha (NCBI), at 1,674,395 to 1,675,927
pntA
Position (kb)
1672
1673
1674
1675 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1672.027 kb on + strand at 1672.082 kb on - strand, within ydgG at 1672.082 kb on - strand, within ydgG at 1672.095 kb on - strand, within ydgG at 1672.095 kb on - strand, within ydgG at 1672.095 kb on - strand, within ydgG at 1672.102 kb on - strand, within ydgG at 1672.106 kb on + strand, within ydgG at 1672.151 kb on - strand, within ydgG at 1672.157 kb on - strand, within ydgG at 1672.291 kb on - strand, within ydgG at 1672.365 kb on + strand, within ydgG at 1672.365 kb on + strand, within ydgG at 1672.413 kb on + strand, within ydgG at 1672.481 kb on + strand, within ydgG at 1672.668 kb on - strand, within ydgG at 1672.698 kb on - strand, within ydgG at 1672.698 kb on - strand, within ydgG at 1672.796 kb on + strand, within ydgG at 1673.042 kb on + strand at 1673.095 kb on - strand at 1673.102 kb on - strand at 1673.145 kb on + strand, within pntB at 1673.501 kb on - strand, within pntB at 1673.579 kb on - strand, within pntB at 1673.579 kb on - strand, within pntB at 1673.667 kb on + strand, within pntB at 1673.672 kb on + strand, within pntB at 1673.672 kb on + strand, within pntB at 1673.878 kb on + strand, within pntB at 1673.878 kb on + strand, within pntB at 1674.216 kb on + strand, within pntB at 1674.370 kb on - strand at 1674.385 kb on + strand at 1674.511 kb on - strand at 1674.521 kb on - strand at 1674.521 kb on - strand at 1674.523 kb on + strand at 1674.583 kb on - strand, within pntA at 1674.865 kb on + strand, within pntA at 1674.894 kb on - strand, within pntA at 1675.064 kb on + strand, within pntA at 1675.064 kb on + strand, within pntA
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 5mM remove 1,672,027 + -1.5 1,672,082 - ydgG b1601 0.14 -2.2 1,672,082 - ydgG b1601 0.14 +0.9 1,672,095 - ydgG b1601 0.15 -1.1 1,672,095 - ydgG b1601 0.15 +2.5 1,672,095 - ydgG b1601 0.15 -0.9 1,672,102 - ydgG b1601 0.16 -2.2 1,672,106 + ydgG b1601 0.16 -2.4 1,672,151 - ydgG b1601 0.21 +2.2 1,672,157 - ydgG b1601 0.21 -2.2 1,672,291 - ydgG b1601 0.34 -1.5 1,672,365 + ydgG b1601 0.41 +1.5 1,672,365 + ydgG b1601 0.41 -2.2 1,672,413 + ydgG b1601 0.46 +0.6 1,672,481 + ydgG b1601 0.53 +1.3 1,672,668 - ydgG b1601 0.71 -2.8 1,672,698 - ydgG b1601 0.74 +0.0 1,672,698 - ydgG b1601 0.74 -2.8 1,672,796 + ydgG b1601 0.83 +2.4 1,673,042 + -0.8 1,673,095 - +0.9 1,673,102 - +0.4 1,673,145 + pntB b1602 0.11 -2.0 1,673,501 - pntB b1602 0.36 +1.5 1,673,579 - pntB b1602 0.42 -0.8 1,673,579 - pntB b1602 0.42 +1.6 1,673,667 + pntB b1602 0.48 +0.6 1,673,672 + pntB b1602 0.49 +3.1 1,673,672 + pntB b1602 0.49 -0.1 1,673,878 + pntB b1602 0.63 +2.3 1,673,878 + pntB b1602 0.63 +0.0 1,674,216 + pntB b1602 0.88 +2.8 1,674,370 - +1.9 1,674,385 + +3.3 1,674,511 - +0.8 1,674,521 - +2.3 1,674,521 - -2.3 1,674,523 + -1.3 1,674,583 - pntA b1603 0.12 -1.3 1,674,865 + pntA b1603 0.31 -0.0 1,674,894 - pntA b1603 0.33 -4.0 1,675,064 + pntA b1603 0.44 -2.8 1,675,064 + pntA b1603 0.44 -1.4
Or see this region's nucleotide sequence