Experiment: carbon source D-Glucose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt paaA and paaB are separated by 11 nucleotides paaB and paaC are separated by 8 nucleotides paaC and paaD are separated by 14 nucleotides paaD and paaE are separated by 7 nucleotides
b1388: paaA - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,451,951 to 1,452,880
paaA
b1389: paaB - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,452,892 to 1,453,179
paaB
b1390: paaC - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,453,188 to 1,453,934
paaC
b1391: paaD - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (RefSeq), at 1,453,949 to 1,454,446
paaD
b1392: paaE - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,454,454 to 1,455,524
paaE
Position (kb)
1453
1454 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1452.190 kb on - strand, within paaA at 1452.425 kb on - strand, within paaA at 1452.529 kb on - strand, within paaA at 1452.529 kb on - strand, within paaA at 1452.655 kb on + strand, within paaA at 1453.221 kb on + strand at 1453.221 kb on + strand at 1453.483 kb on - strand, within paaC at 1453.635 kb on - strand, within paaC at 1453.641 kb on + strand, within paaC at 1453.689 kb on + strand, within paaC at 1453.840 kb on - strand, within paaC at 1454.381 kb on + strand, within paaD at 1454.381 kb on + strand, within paaD at 1454.398 kb on - strand at 1454.470 kb on + strand at 1454.532 kb on - strand at 1454.714 kb on + strand, within paaE at 1454.714 kb on + strand, within paaE at 1454.786 kb on - strand, within paaE
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 5mM remove 1,452,190 - paaA b1388 0.26 -0.5 1,452,425 - paaA b1388 0.51 +1.7 1,452,529 - paaA b1388 0.62 -0.4 1,452,529 - paaA b1388 0.62 +1.7 1,452,655 + paaA b1388 0.76 +0.6 1,453,221 + +0.5 1,453,221 + -0.2 1,453,483 - paaC b1390 0.39 +0.4 1,453,635 - paaC b1390 0.60 +1.4 1,453,641 + paaC b1390 0.61 -0.5 1,453,689 + paaC b1390 0.67 +1.6 1,453,840 - paaC b1390 0.87 +2.0 1,454,381 + paaD b1391 0.87 -3.8 1,454,381 + paaD b1391 0.87 -3.0 1,454,398 - +0.9 1,454,470 + +0.5 1,454,532 - -3.5 1,454,714 + paaE b1392 0.24 +0.4 1,454,714 + paaE b1392 0.24 +0.6 1,454,786 - paaE b1392 0.31 -0.4
Or see this region's nucleotide sequence