Experiment: carbon source D-Mannose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lplA and ytjB are separated by 27 nucleotides ytjB and serB are separated by 105 nucleotides serB and radA are separated by 48 nucleotides
b4386: lplA - lipoate-protein ligase A (NCBI), at 4,621,124 to 4,622,140
lplA
b4387: ytjB - hypothetical protein (NCBI), at 4,622,168 to 4,622,812
ytjB
b4388: serB - 3-phosphoserine phosphatase (NCBI), at 4,622,918 to 4,623,886
serB
b4389: radA - predicted repair protein (NCBI), at 4,623,935 to 4,625,317
radA
Position (kb)
4622
4623
4624 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 4621.946 kb on - strand, within lplA at 4621.946 kb on - strand, within lplA at 4621.952 kb on - strand, within lplA at 4621.957 kb on - strand, within lplA at 4621.970 kb on + strand, within lplA at 4621.970 kb on + strand, within lplA at 4621.976 kb on - strand, within lplA at 4622.011 kb on - strand, within lplA at 4622.011 kb on - strand, within lplA at 4622.126 kb on + strand at 4622.281 kb on - strand, within ytjB at 4622.281 kb on - strand, within ytjB at 4622.284 kb on - strand, within ytjB at 4622.284 kb on - strand, within ytjB at 4622.289 kb on - strand, within ytjB at 4622.305 kb on + strand, within ytjB at 4622.531 kb on + strand, within ytjB at 4622.534 kb on + strand, within ytjB at 4622.539 kb on - strand, within ytjB at 4622.539 kb on - strand, within ytjB at 4622.637 kb on - strand, within ytjB at 4622.653 kb on + strand, within ytjB at 4622.705 kb on - strand, within ytjB at 4622.816 kb on - strand at 4622.846 kb on + strand at 4622.901 kb on + strand at 4622.901 kb on + strand at 4622.925 kb on + strand at 4622.925 kb on + strand at 4622.925 kb on + strand at 4622.944 kb on + strand at 4622.952 kb on - strand at 4622.952 kb on - strand at 4622.977 kb on - strand at 4623.023 kb on + strand, within serB at 4623.023 kb on - strand, within serB at 4623.038 kb on + strand, within serB at 4623.038 kb on + strand, within serB at 4623.048 kb on + strand, within serB at 4623.048 kb on + strand, within serB at 4623.056 kb on - strand, within serB at 4623.109 kb on - strand, within serB at 4623.126 kb on - strand, within serB at 4623.126 kb on - strand, within serB at 4623.202 kb on + strand, within serB at 4623.296 kb on + strand, within serB at 4623.352 kb on - strand, within serB at 4623.352 kb on - strand, within serB at 4623.551 kb on + strand, within serB at 4623.691 kb on + strand, within serB at 4623.691 kb on + strand, within serB at 4623.746 kb on - strand, within serB at 4623.832 kb on + strand at 4623.832 kb on + strand at 4623.860 kb on + strand at 4623.895 kb on + strand at 4623.895 kb on + strand at 4623.910 kb on - strand at 4623.925 kb on - strand at 4623.925 kb on - strand at 4623.933 kb on - strand at 4624.047 kb on + strand at 4624.047 kb on + strand at 4624.050 kb on + strand at 4624.104 kb on - strand, within radA at 4624.104 kb on - strand, within radA at 4624.121 kb on + strand, within radA at 4624.173 kb on + strand, within radA at 4624.173 kb on + strand, within radA at 4624.173 kb on + strand, within radA at 4624.181 kb on - strand, within radA at 4624.181 kb on - strand, within radA at 4624.211 kb on + strand, within radA at 4624.219 kb on - strand, within radA at 4624.334 kb on + strand, within radA at 4624.405 kb on - strand, within radA at 4624.405 kb on - strand, within radA at 4624.422 kb on + strand, within radA at 4624.490 kb on + strand, within radA at 4624.490 kb on + strand, within radA at 4624.498 kb on - strand, within radA at 4624.532 kb on - strand, within radA at 4624.569 kb on + strand, within radA at 4624.620 kb on + strand, within radA at 4624.648 kb on - strand, within radA at 4624.755 kb on + strand, within radA at 4624.755 kb on + strand, within radA at 4624.826 kb on + strand, within radA at 4624.826 kb on + strand, within radA at 4624.827 kb on + strand, within radA at 4624.832 kb on + strand, within radA at 4624.855 kb on - strand, within radA at 4624.864 kb on + strand, within radA
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Mannose 20mM remove 4,621,946 - lplA b4386 0.81 +0.1 4,621,946 - lplA b4386 0.81 -0.4 4,621,952 - lplA b4386 0.81 -0.9 4,621,957 - lplA b4386 0.82 +0.2 4,621,970 + lplA b4386 0.83 -0.1 4,621,970 + lplA b4386 0.83 -0.3 4,621,976 - lplA b4386 0.84 -0.2 4,622,011 - lplA b4386 0.87 +0.8 4,622,011 - lplA b4386 0.87 +0.1 4,622,126 + -0.1 4,622,281 - ytjB b4387 0.18 +0.3 4,622,281 - ytjB b4387 0.18 +0.7 4,622,284 - ytjB b4387 0.18 +1.4 4,622,284 - ytjB b4387 0.18 -1.1 4,622,289 - ytjB b4387 0.19 -0.6 4,622,305 + ytjB b4387 0.21 +0.3 4,622,531 + ytjB b4387 0.56 -1.1 4,622,534 + ytjB b4387 0.57 -0.2 4,622,539 - ytjB b4387 0.58 -1.6 4,622,539 - ytjB b4387 0.58 +1.7 4,622,637 - ytjB b4387 0.73 +0.9 4,622,653 + ytjB b4387 0.75 +0.1 4,622,705 - ytjB b4387 0.83 -0.3 4,622,816 - +0.2 4,622,846 + -0.1 4,622,901 + -1.5 4,622,901 + -0.1 4,622,925 + -2.2 4,622,925 + -1.3 4,622,925 + -4.7 4,622,944 + +0.1 4,622,952 - -3.9 4,622,952 - -4.2 4,622,977 - -5.8 4,623,023 + serB b4388 0.11 -2.5 4,623,023 - serB b4388 0.11 -4.4 4,623,038 + serB b4388 0.12 -2.6 4,623,038 + serB b4388 0.12 -2.7 4,623,048 + serB b4388 0.13 -2.8 4,623,048 + serB b4388 0.13 -2.9 4,623,056 - serB b4388 0.14 -5.5 4,623,109 - serB b4388 0.20 -3.4 4,623,126 - serB b4388 0.21 -1.9 4,623,126 - serB b4388 0.21 -3.6 4,623,202 + serB b4388 0.29 -6.2 4,623,296 + serB b4388 0.39 -4.9 4,623,352 - serB b4388 0.45 -2.5 4,623,352 - serB b4388 0.45 -1.1 4,623,551 + serB b4388 0.65 -5.0 4,623,691 + serB b4388 0.80 -4.1 4,623,691 + serB b4388 0.80 -5.0 4,623,746 - serB b4388 0.85 -2.2 4,623,832 + -3.6 4,623,832 + -2.9 4,623,860 + -2.5 4,623,895 + -0.3 4,623,895 + +0.4 4,623,910 - -0.1 4,623,925 - +0.1 4,623,925 - +1.2 4,623,933 - +0.9 4,624,047 + -1.3 4,624,047 + +0.1 4,624,050 + +0.2 4,624,104 - radA b4389 0.12 +0.6 4,624,104 - radA b4389 0.12 +0.8 4,624,121 + radA b4389 0.13 -2.2 4,624,173 + radA b4389 0.17 +0.1 4,624,173 + radA b4389 0.17 -1.0 4,624,173 + radA b4389 0.17 +1.0 4,624,181 - radA b4389 0.18 -2.3 4,624,181 - radA b4389 0.18 +0.4 4,624,211 + radA b4389 0.20 -0.3 4,624,219 - radA b4389 0.21 +0.9 4,624,334 + radA b4389 0.29 -0.7 4,624,405 - radA b4389 0.34 -1.2 4,624,405 - radA b4389 0.34 -0.0 4,624,422 + radA b4389 0.35 -0.6 4,624,490 + radA b4389 0.40 -1.6 4,624,490 + radA b4389 0.40 -1.3 4,624,498 - radA b4389 0.41 +0.6 4,624,532 - radA b4389 0.43 -2.2 4,624,569 + radA b4389 0.46 -0.4 4,624,620 + radA b4389 0.50 -2.1 4,624,648 - radA b4389 0.52 +0.0 4,624,755 + radA b4389 0.59 -1.9 4,624,755 + radA b4389 0.59 -0.1 4,624,826 + radA b4389 0.64 +0.1 4,624,826 + radA b4389 0.64 -0.1 4,624,827 + radA b4389 0.64 -0.9 4,624,832 + radA b4389 0.65 +0.1 4,624,855 - radA b4389 0.67 +0.3 4,624,864 + radA b4389 0.67 -0.1
Or see this region's nucleotide sequence