Strain Fitness in Escherichia coli BW25113 around b4220

Experiment: carbon source D-Mannose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmsrA and ytfM are separated by 205 nucleotidesytfM and ytfN overlap by 4 nucleotides b4219: msrA - methionine sulfoxide reductase A (NCBI), at 4,439,561 to 4,440,199 msrA b4220: ytfM - predicted outer membrane protein and surface antigen (NCBI), at 4,440,405 to 4,442,138 ytfM b4221: ytfN - hypothetical protein (NCBI), at 4,442,135 to 4,445,914 ytfN Position (kb) 4440 4441 4442 4443Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4439.431 kb on - strandat 4439.431 kb on - strandat 4439.466 kb on - strandat 4439.688 kb on + strand, within msrAat 4439.739 kb on + strand, within msrAat 4439.759 kb on + strand, within msrAat 4439.759 kb on + strand, within msrAat 4439.764 kb on + strand, within msrAat 4439.872 kb on + strand, within msrAat 4439.937 kb on - strand, within msrAat 4439.937 kb on - strand, within msrAat 4440.009 kb on + strand, within msrAat 4440.090 kb on + strand, within msrAat 4440.120 kb on - strand, within msrAat 4440.131 kb on - strand, within msrAat 4440.143 kb on - strandat 4440.143 kb on - strandat 4440.279 kb on - strandat 4440.331 kb on + strandat 4440.331 kb on + strandat 4440.430 kb on + strandat 4440.435 kb on + strandat 4440.435 kb on + strandat 4440.435 kb on + strandat 4440.483 kb on - strandat 4440.483 kb on - strandat 4440.485 kb on - strandat 4440.582 kb on + strand, within ytfMat 4440.582 kb on - strand, within ytfMat 4440.675 kb on - strand, within ytfMat 4440.681 kb on + strand, within ytfMat 4440.698 kb on + strand, within ytfMat 4440.740 kb on + strand, within ytfMat 4440.741 kb on - strand, within ytfMat 4440.741 kb on - strand, within ytfMat 4440.770 kb on - strand, within ytfMat 4440.770 kb on - strand, within ytfMat 4440.798 kb on + strand, within ytfMat 4440.810 kb on + strand, within ytfMat 4440.810 kb on + strand, within ytfMat 4440.810 kb on + strand, within ytfMat 4440.810 kb on + strand, within ytfMat 4440.810 kb on + strand, within ytfMat 4440.818 kb on - strand, within ytfMat 4440.861 kb on + strand, within ytfMat 4440.864 kb on - strand, within ytfMat 4440.864 kb on - strand, within ytfMat 4440.935 kb on + strand, within ytfMat 4440.935 kb on + strand, within ytfMat 4440.948 kb on + strand, within ytfMat 4440.973 kb on + strand, within ytfMat 4440.976 kb on - strand, within ytfMat 4440.989 kb on + strand, within ytfMat 4440.996 kb on - strand, within ytfMat 4441.096 kb on + strand, within ytfMat 4441.144 kb on - strand, within ytfMat 4441.234 kb on - strand, within ytfMat 4441.234 kb on - strand, within ytfMat 4441.292 kb on + strand, within ytfMat 4441.306 kb on + strand, within ytfMat 4441.382 kb on + strand, within ytfMat 4441.406 kb on + strand, within ytfMat 4441.406 kb on + strand, within ytfMat 4441.416 kb on + strand, within ytfMat 4441.424 kb on - strand, within ytfMat 4441.441 kb on + strand, within ytfMat 4441.441 kb on + strand, within ytfMat 4441.442 kb on + strand, within ytfMat 4441.456 kb on + strand, within ytfMat 4441.458 kb on + strand, within ytfMat 4441.495 kb on + strand, within ytfMat 4441.503 kb on - strand, within ytfMat 4441.521 kb on - strand, within ytfMat 4441.559 kb on + strand, within ytfMat 4441.564 kb on + strand, within ytfMat 4441.564 kb on + strand, within ytfMat 4441.567 kb on - strand, within ytfMat 4441.614 kb on + strand, within ytfMat 4441.614 kb on + strand, within ytfMat 4441.639 kb on + strand, within ytfMat 4441.639 kb on + strand, within ytfMat 4441.653 kb on + strand, within ytfMat 4441.657 kb on - strand, within ytfMat 4441.657 kb on - strand, within ytfMat 4441.657 kb on - strand, within ytfMat 4441.717 kb on + strand, within ytfMat 4441.727 kb on - strand, within ytfMat 4441.828 kb on + strand, within ytfMat 4441.933 kb on - strand, within ytfMat 4441.933 kb on - strand, within ytfMat 4441.969 kb on - strandat 4441.969 kb on - strandat 4442.033 kb on - strandat 4442.108 kb on + strandat 4442.183 kb on + strandat 4442.201 kb on - strandat 4442.225 kb on + strandat 4442.262 kb on + strandat 4442.262 kb on + strandat 4442.264 kb on - strandat 4442.303 kb on + strandat 4442.303 kb on + strandat 4442.312 kb on - strandat 4442.400 kb on + strandat 4442.448 kb on - strandat 4442.448 kb on - strandat 4442.469 kb on + strandat 4442.469 kb on + strandat 4442.512 kb on - strandat 4442.515 kb on + strand, within ytfNat 4442.535 kb on + strand, within ytfNat 4442.535 kb on + strand, within ytfNat 4442.542 kb on - strand, within ytfNat 4442.543 kb on - strand, within ytfNat 4442.544 kb on + strand, within ytfNat 4442.544 kb on + strand, within ytfNat 4442.549 kb on + strand, within ytfNat 4442.557 kb on - strand, within ytfNat 4442.557 kb on - strand, within ytfNat 4442.557 kb on - strand, within ytfNat 4442.571 kb on + strand, within ytfNat 4442.571 kb on + strand, within ytfNat 4442.594 kb on - strand, within ytfNat 4442.634 kb on + strand, within ytfNat 4442.634 kb on + strand, within ytfNat 4442.704 kb on + strand, within ytfNat 4442.704 kb on + strand, within ytfNat 4442.818 kb on - strand, within ytfNat 4442.853 kb on - strand, within ytfNat 4442.853 kb on - strand, within ytfNat 4442.855 kb on + strand, within ytfNat 4442.872 kb on + strand, within ytfNat 4442.890 kb on + strand, within ytfNat 4442.890 kb on + strand, within ytfNat 4442.912 kb on - strand, within ytfNat 4443.017 kb on + strand, within ytfNat 4443.017 kb on + strand, within ytfNat 4443.031 kb on + strand, within ytfNat 4443.033 kb on + strand, within ytfNat 4443.041 kb on - strand, within ytfNat 4443.041 kb on - strand, within ytfNat 4443.041 kb on - strand, within ytfNat 4443.041 kb on - strand, within ytfNat 4443.053 kb on - strand, within ytfNat 4443.053 kb on - strand, within ytfNat 4443.106 kb on + strand, within ytfN

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 20mM
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4,439,431 - -0.4
4,439,431 - -0.5
4,439,466 - -1.0
4,439,688 + msrA b4219 0.20 +0.1
4,439,739 + msrA b4219 0.28 +0.1
4,439,759 + msrA b4219 0.31 -2.2
4,439,759 + msrA b4219 0.31 +0.3
4,439,764 + msrA b4219 0.32 -0.1
4,439,872 + msrA b4219 0.49 -0.2
4,439,937 - msrA b4219 0.59 +1.2
4,439,937 - msrA b4219 0.59 -0.3
4,440,009 + msrA b4219 0.70 +0.7
4,440,090 + msrA b4219 0.83 +0.7
4,440,120 - msrA b4219 0.87 +0.9
4,440,131 - msrA b4219 0.89 +0.5
4,440,143 - -1.1
4,440,143 - -0.1
4,440,279 - -0.3
4,440,331 + +0.4
4,440,331 + +1.3
4,440,430 + +1.1
4,440,435 + +0.3
4,440,435 + +0.7
4,440,435 + +0.6
4,440,483 - +1.2
4,440,483 - +1.0
4,440,485 - -0.1
4,440,582 + ytfM b4220 0.10 +0.3
4,440,582 - ytfM b4220 0.10 +0.8
4,440,675 - ytfM b4220 0.16 +1.4
4,440,681 + ytfM b4220 0.16 +2.3
4,440,698 + ytfM b4220 0.17 +0.8
4,440,740 + ytfM b4220 0.19 +0.8
4,440,741 - ytfM b4220 0.19 +1.0
4,440,741 - ytfM b4220 0.19 +0.2
4,440,770 - ytfM b4220 0.21 +0.3
4,440,770 - ytfM b4220 0.21 -0.5
4,440,798 + ytfM b4220 0.23 +1.0
4,440,810 + ytfM b4220 0.23 +2.0
4,440,810 + ytfM b4220 0.23 -2.5
4,440,810 + ytfM b4220 0.23 +0.8
4,440,810 + ytfM b4220 0.23 +1.3
4,440,810 + ytfM b4220 0.23 +0.3
4,440,818 - ytfM b4220 0.24 +0.9
4,440,861 + ytfM b4220 0.26 +1.7
4,440,864 - ytfM b4220 0.26 +1.4
4,440,864 - ytfM b4220 0.26 +2.5
4,440,935 + ytfM b4220 0.31 +1.0
4,440,935 + ytfM b4220 0.31 +0.1
4,440,948 + ytfM b4220 0.31 +0.7
4,440,973 + ytfM b4220 0.33 +0.3
4,440,976 - ytfM b4220 0.33 +0.8
4,440,989 + ytfM b4220 0.34 -0.3
4,440,996 - ytfM b4220 0.34 +0.8
4,441,096 + ytfM b4220 0.40 -0.3
4,441,144 - ytfM b4220 0.43 +0.5
4,441,234 - ytfM b4220 0.48 -0.2
4,441,234 - ytfM b4220 0.48 +0.1
4,441,292 + ytfM b4220 0.51 +0.3
4,441,306 + ytfM b4220 0.52 +0.8
4,441,382 + ytfM b4220 0.56 +1.1
4,441,406 + ytfM b4220 0.58 +0.7
4,441,406 + ytfM b4220 0.58 +1.4
4,441,416 + ytfM b4220 0.58 +0.6
4,441,424 - ytfM b4220 0.59 -0.1
4,441,441 + ytfM b4220 0.60 +1.2
4,441,441 + ytfM b4220 0.60 -0.3
4,441,442 + ytfM b4220 0.60 +1.2
4,441,456 + ytfM b4220 0.61 +0.7
4,441,458 + ytfM b4220 0.61 +0.7
4,441,495 + ytfM b4220 0.63 +0.6
4,441,503 - ytfM b4220 0.63 +0.9
4,441,521 - ytfM b4220 0.64 +0.2
4,441,559 + ytfM b4220 0.67 +1.1
4,441,564 + ytfM b4220 0.67 +1.0
4,441,564 + ytfM b4220 0.67 +1.0
4,441,567 - ytfM b4220 0.67 +0.3
4,441,614 + ytfM b4220 0.70 +0.9
4,441,614 + ytfM b4220 0.70 +0.3
4,441,639 + ytfM b4220 0.71 +1.3
4,441,639 + ytfM b4220 0.71 +0.2
4,441,653 + ytfM b4220 0.72 +1.0
4,441,657 - ytfM b4220 0.72 +1.7
4,441,657 - ytfM b4220 0.72 +0.5
4,441,657 - ytfM b4220 0.72 +0.8
4,441,717 + ytfM b4220 0.76 +1.1
4,441,727 - ytfM b4220 0.76 +0.5
4,441,828 + ytfM b4220 0.82 +1.3
4,441,933 - ytfM b4220 0.88 +0.7
4,441,933 - ytfM b4220 0.88 +0.5
4,441,969 - +1.3
4,441,969 - +0.7
4,442,033 - +0.7
4,442,108 + +1.8
4,442,183 + +0.3
4,442,201 - +2.4
4,442,225 + +0.4
4,442,262 + -0.3
4,442,262 + +0.6
4,442,264 - -0.8
4,442,303 + +1.4
4,442,303 + -0.4
4,442,312 - +0.6
4,442,400 + +0.9
4,442,448 - -0.2
4,442,448 - +0.5
4,442,469 + +0.6
4,442,469 + +0.9
4,442,512 - +1.2
4,442,515 + ytfN b4221 0.10 +0.0
4,442,535 + ytfN b4221 0.11 +0.6
4,442,535 + ytfN b4221 0.11 +0.7
4,442,542 - ytfN b4221 0.11 +0.3
4,442,543 - ytfN b4221 0.11 +0.2
4,442,544 + ytfN b4221 0.11 +1.1
4,442,544 + ytfN b4221 0.11 +0.7
4,442,549 + ytfN b4221 0.11 +0.8
4,442,557 - ytfN b4221 0.11 -0.4
4,442,557 - ytfN b4221 0.11 +1.3
4,442,557 - ytfN b4221 0.11 +0.5
4,442,571 + ytfN b4221 0.12 +0.2
4,442,571 + ytfN b4221 0.12 +0.3
4,442,594 - ytfN b4221 0.12 +0.7
4,442,634 + ytfN b4221 0.13 -0.4
4,442,634 + ytfN b4221 0.13 +1.5
4,442,704 + ytfN b4221 0.15 +1.1
4,442,704 + ytfN b4221 0.15 +0.5
4,442,818 - ytfN b4221 0.18 +1.0
4,442,853 - ytfN b4221 0.19 +1.2
4,442,853 - ytfN b4221 0.19 +0.6
4,442,855 + ytfN b4221 0.19 -0.4
4,442,872 + ytfN b4221 0.19 +3.3
4,442,890 + ytfN b4221 0.20 +0.9
4,442,890 + ytfN b4221 0.20 +0.1
4,442,912 - ytfN b4221 0.21 +0.8
4,443,017 + ytfN b4221 0.23 +1.1
4,443,017 + ytfN b4221 0.23 +1.8
4,443,031 + ytfN b4221 0.24 +0.9
4,443,033 + ytfN b4221 0.24 +0.3
4,443,041 - ytfN b4221 0.24 +0.9
4,443,041 - ytfN b4221 0.24 +0.7
4,443,041 - ytfN b4221 0.24 +1.1
4,443,041 - ytfN b4221 0.24 +0.6
4,443,053 - ytfN b4221 0.24 +1.3
4,443,053 - ytfN b4221 0.24 +0.9
4,443,106 + ytfN b4221 0.26 +0.8

Or see this region's nucleotide sequence