Experiment: carbon source D-Mannose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt arpA and iclR are separated by 316 nucleotides iclR and metH are separated by 199 nucleotides
b4017: arpA - regulator of acetyl CoA synthetase (NCBI), at 4,218,324 to 4,220,510
arpA
b4018: iclR - repressor of aceBA operon (VIMSS), at 4,220,827 to 4,221,651
iclR
b4019: metH - B12-dependent methionine synthase (NCBI), at 4,221,851 to 4,225,534
metH
Position (kb)
4220
4221
4222 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 4219.879 kb on + strand, within arpA at 4219.892 kb on + strand, within arpA at 4219.892 kb on + strand, within arpA at 4219.895 kb on + strand, within arpA at 4219.900 kb on - strand, within arpA at 4219.902 kb on + strand, within arpA at 4219.995 kb on + strand, within arpA at 4220.048 kb on + strand, within arpA at 4220.050 kb on + strand, within arpA at 4220.050 kb on + strand, within arpA at 4220.051 kb on + strand, within arpA at 4220.058 kb on + strand, within arpA at 4220.058 kb on + strand, within arpA at 4220.603 kb on - strand at 4220.630 kb on - strand at 4220.728 kb on - strand at 4220.730 kb on - strand at 4220.730 kb on - strand at 4220.759 kb on - strand at 4220.783 kb on - strand at 4220.815 kb on - strand at 4220.815 kb on - strand at 4220.840 kb on - strand at 4220.858 kb on - strand at 4220.872 kb on + strand at 4220.883 kb on + strand at 4220.907 kb on + strand at 4220.960 kb on - strand, within iclR at 4220.969 kb on + strand, within iclR at 4220.971 kb on + strand, within iclR at 4220.992 kb on - strand, within iclR at 4220.992 kb on - strand, within iclR at 4220.994 kb on + strand, within iclR at 4220.997 kb on + strand, within iclR at 4221.000 kb on + strand, within iclR at 4221.001 kb on - strand, within iclR at 4221.013 kb on - strand, within iclR at 4221.064 kb on + strand, within iclR at 4221.089 kb on - strand, within iclR at 4221.094 kb on - strand, within iclR at 4221.094 kb on - strand, within iclR at 4221.099 kb on - strand, within iclR at 4221.100 kb on + strand, within iclR at 4221.100 kb on + strand, within iclR at 4221.100 kb on - strand, within iclR at 4221.127 kb on + strand, within iclR at 4221.140 kb on - strand, within iclR at 4221.140 kb on - strand, within iclR at 4221.186 kb on - strand, within iclR at 4221.227 kb on + strand, within iclR at 4221.227 kb on + strand, within iclR at 4221.232 kb on + strand, within iclR at 4221.234 kb on + strand, within iclR at 4221.234 kb on + strand, within iclR at 4221.235 kb on - strand, within iclR at 4221.235 kb on - strand, within iclR at 4221.277 kb on - strand, within iclR at 4221.277 kb on - strand, within iclR at 4221.320 kb on - strand, within iclR at 4221.330 kb on - strand, within iclR at 4221.335 kb on - strand, within iclR at 4221.452 kb on + strand, within iclR at 4221.524 kb on - strand, within iclR at 4221.585 kb on + strand at 4221.861 kb on + strand at 4221.920 kb on - strand at 4221.922 kb on - strand at 4221.936 kb on + strand at 4221.936 kb on + strand at 4222.014 kb on + strand at 4222.049 kb on + strand at 4222.057 kb on - strand at 4222.068 kb on - strand at 4222.196 kb on + strand at 4222.198 kb on + strand at 4222.215 kb on + strand at 4222.215 kb on + strand at 4222.215 kb on + strand at 4222.270 kb on + strand, within metH at 4222.335 kb on + strand, within metH at 4222.335 kb on + strand, within metH at 4222.389 kb on - strand, within metH at 4222.447 kb on + strand, within metH at 4222.455 kb on - strand, within metH at 4222.455 kb on - strand, within metH at 4222.468 kb on - strand, within metH at 4222.519 kb on + strand, within metH at 4222.549 kb on - strand, within metH at 4222.630 kb on - strand, within metH
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Mannose 20mM remove 4,219,879 + arpA b4017 0.71 -0.7 4,219,892 + arpA b4017 0.72 +1.5 4,219,892 + arpA b4017 0.72 +2.5 4,219,895 + arpA b4017 0.72 +2.5 4,219,900 - arpA b4017 0.72 +2.5 4,219,902 + arpA b4017 0.72 +2.5 4,219,995 + arpA b4017 0.76 +2.5 4,220,048 + arpA b4017 0.79 +2.5 4,220,050 + arpA b4017 0.79 +0.5 4,220,050 + arpA b4017 0.79 +2.5 4,220,051 + arpA b4017 0.79 +2.5 4,220,058 + arpA b4017 0.79 +2.5 4,220,058 + arpA b4017 0.79 -0.5 4,220,603 - -0.6 4,220,630 - +0.9 4,220,728 - -1.2 4,220,730 - -0.8 4,220,730 - -3.3 4,220,759 - +0.8 4,220,783 - -0.2 4,220,815 - -0.9 4,220,815 - +0.8 4,220,840 - -0.7 4,220,858 - -0.9 4,220,872 + -1.7 4,220,883 + -0.1 4,220,907 + -0.1 4,220,960 - iclR b4018 0.16 +0.1 4,220,969 + iclR b4018 0.17 +0.7 4,220,971 + iclR b4018 0.17 +1.5 4,220,992 - iclR b4018 0.20 +0.1 4,220,992 - iclR b4018 0.20 -2.0 4,220,994 + iclR b4018 0.20 -0.8 4,220,997 + iclR b4018 0.21 -3.2 4,221,000 + iclR b4018 0.21 -2.7 4,221,001 - iclR b4018 0.21 -2.3 4,221,013 - iclR b4018 0.23 -2.0 4,221,064 + iclR b4018 0.29 +4.3 4,221,089 - iclR b4018 0.32 -1.8 4,221,094 - iclR b4018 0.32 +0.1 4,221,094 - iclR b4018 0.32 -1.3 4,221,099 - iclR b4018 0.33 -1.1 4,221,100 + iclR b4018 0.33 -0.8 4,221,100 + iclR b4018 0.33 -1.5 4,221,100 - iclR b4018 0.33 -1.2 4,221,127 + iclR b4018 0.36 -0.7 4,221,140 - iclR b4018 0.38 -0.3 4,221,140 - iclR b4018 0.38 -2.4 4,221,186 - iclR b4018 0.44 -0.5 4,221,227 + iclR b4018 0.48 -2.9 4,221,227 + iclR b4018 0.48 -4.1 4,221,232 + iclR b4018 0.49 +1.0 4,221,234 + iclR b4018 0.49 -2.4 4,221,234 + iclR b4018 0.49 -0.3 4,221,235 - iclR b4018 0.49 -2.9 4,221,235 - iclR b4018 0.49 -1.5 4,221,277 - iclR b4018 0.55 -1.5 4,221,277 - iclR b4018 0.55 -0.7 4,221,320 - iclR b4018 0.60 -2.9 4,221,330 - iclR b4018 0.61 -0.5 4,221,335 - iclR b4018 0.62 -0.8 4,221,452 + iclR b4018 0.76 +0.5 4,221,524 - iclR b4018 0.84 -2.2 4,221,585 + -3.3 4,221,861 + +0.9 4,221,920 - +0.5 4,221,922 - -0.3 4,221,936 + -1.1 4,221,936 + -1.7 4,222,014 + +0.1 4,222,049 + -0.6 4,222,057 - +0.1 4,222,068 - -1.1 4,222,196 + +0.5 4,222,198 + -2.2 4,222,215 + -0.3 4,222,215 + +0.1 4,222,215 + +0.8 4,222,270 + metH b4019 0.11 -0.6 4,222,335 + metH b4019 0.13 -0.4 4,222,335 + metH b4019 0.13 +0.4 4,222,389 - metH b4019 0.15 -0.7 4,222,447 + metH b4019 0.16 -2.8 4,222,455 - metH b4019 0.16 -2.6 4,222,455 - metH b4019 0.16 -0.1 4,222,468 - metH b4019 0.17 -0.3 4,222,519 + metH b4019 0.18 +0.1 4,222,549 - metH b4019 0.19 +0.1 4,222,630 - metH b4019 0.21 +0.8
Or see this region's nucleotide sequence