Experiment: carbon source D-Mannose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rhsB and yhhH overlap by 29 nucleotides yhhH and yrhC are separated by 104 nucleotides yrhC and yhhI are separated by 140 nucleotides yhhI and yhhJ are separated by 164 nucleotides
b3482: rhsB - rhsB element core protein RshB (NCBI), at 3,617,215 to 3,621,450
rhsB
b3483: yhhH - orf, hypothetical protein (VIMSS), at 3,621,422 to 3,621,805
yhhH
b4552: yrhC - no description, at 3,621,910 to 3,622,260
yrhC
b3484: yhhI - predicted transposase (NCBI), at 3,622,401 to 3,623,537
yhhI
b3485: yhhJ - predicted transporter subunit: membrane component of ABC superfamily (RefSeq), at 3,623,702 to 3,624,826
yhhJ
Position (kb)
3622
3623
3624 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3621.654 kb on - strand, within yhhH at 3621.654 kb on - strand, within yhhH at 3621.816 kb on - strand at 3621.827 kb on - strand at 3621.836 kb on - strand at 3621.848 kb on - strand at 3621.848 kb on - strand at 3621.851 kb on + strand at 3621.852 kb on - strand at 3621.853 kb on - strand at 3621.858 kb on + strand at 3621.858 kb on + strand at 3621.858 kb on + strand at 3621.866 kb on + strand at 3622.073 kb on - strand, within yrhC at 3622.075 kb on - strand, within yrhC at 3622.075 kb on - strand, within yrhC at 3622.262 kb on - strand at 3622.360 kb on + strand at 3622.375 kb on + strand at 3622.375 kb on + strand at 3623.245 kb on + strand, within yhhI at 3623.298 kb on - strand, within yhhI at 3623.298 kb on - strand, within yhhI at 3623.318 kb on - strand, within yhhI at 3623.325 kb on - strand, within yhhI at 3623.337 kb on - strand, within yhhI at 3623.337 kb on - strand, within yhhI at 3623.340 kb on - strand, within yhhI at 3623.542 kb on + strand at 3623.679 kb on - strand at 3623.679 kb on - strand at 3623.681 kb on - strand at 3623.681 kb on - strand at 3623.733 kb on - strand at 3623.841 kb on + strand, within yhhJ at 3623.864 kb on + strand, within yhhJ at 3623.869 kb on + strand, within yhhJ at 3623.874 kb on + strand, within yhhJ at 3623.874 kb on + strand, within yhhJ at 3623.876 kb on + strand, within yhhJ at 3623.906 kb on - strand, within yhhJ at 3623.916 kb on - strand, within yhhJ at 3623.916 kb on - strand, within yhhJ at 3623.969 kb on - strand, within yhhJ at 3624.083 kb on + strand, within yhhJ at 3624.085 kb on + strand, within yhhJ at 3624.112 kb on + strand, within yhhJ at 3624.130 kb on + strand, within yhhJ at 3624.200 kb on + strand, within yhhJ at 3624.215 kb on + strand, within yhhJ at 3624.235 kb on + strand, within yhhJ at 3624.238 kb on - strand, within yhhJ at 3624.238 kb on - strand, within yhhJ at 3624.238 kb on - strand, within yhhJ at 3624.256 kb on - strand, within yhhJ at 3624.256 kb on - strand, within yhhJ at 3624.273 kb on - strand, within yhhJ at 3624.317 kb on - strand, within yhhJ at 3624.317 kb on - strand, within yhhJ at 3624.365 kb on + strand, within yhhJ at 3624.407 kb on + strand, within yhhJ at 3624.407 kb on + strand, within yhhJ at 3624.448 kb on - strand, within yhhJ at 3624.489 kb on + strand, within yhhJ
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Mannose 20mM remove 3,621,654 - yhhH b3483 0.60 +2.5 3,621,654 - yhhH b3483 0.60 +2.5 3,621,816 - +1.1 3,621,827 - -0.5 3,621,836 - +2.5 3,621,848 - +0.9 3,621,848 - +2.5 3,621,851 + +2.5 3,621,852 - +2.5 3,621,853 - +2.5 3,621,858 + +2.5 3,621,858 + +0.2 3,621,858 + +2.5 3,621,866 + +2.5 3,622,073 - yrhC b4552 0.46 +2.5 3,622,075 - yrhC b4552 0.47 +2.5 3,622,075 - yrhC b4552 0.47 +1.0 3,622,262 - -0.9 3,622,360 + +0.5 3,622,375 + -0.3 3,622,375 + +2.5 3,623,245 + yhhI b3484 0.74 -0.8 3,623,298 - yhhI b3484 0.79 -0.0 3,623,298 - yhhI b3484 0.79 -2.1 3,623,318 - yhhI b3484 0.81 -1.4 3,623,325 - yhhI b3484 0.81 -0.2 3,623,337 - yhhI b3484 0.82 -1.3 3,623,337 - yhhI b3484 0.82 -2.0 3,623,340 - yhhI b3484 0.83 -0.5 3,623,542 + +0.5 3,623,679 - +0.5 3,623,679 - +1.0 3,623,681 - +0.0 3,623,681 - -1.1 3,623,733 - +1.0 3,623,841 + yhhJ b3485 0.12 -0.4 3,623,864 + yhhJ b3485 0.14 +0.5 3,623,869 + yhhJ b3485 0.15 -3.1 3,623,874 + yhhJ b3485 0.15 +0.3 3,623,874 + yhhJ b3485 0.15 -3.2 3,623,876 + yhhJ b3485 0.15 +0.3 3,623,906 - yhhJ b3485 0.18 -2.1 3,623,916 - yhhJ b3485 0.19 -0.4 3,623,916 - yhhJ b3485 0.19 +0.7 3,623,969 - yhhJ b3485 0.24 +0.3 3,624,083 + yhhJ b3485 0.34 -1.3 3,624,085 + yhhJ b3485 0.34 +0.9 3,624,112 + yhhJ b3485 0.36 +1.2 3,624,130 + yhhJ b3485 0.38 -1.8 3,624,200 + yhhJ b3485 0.44 +0.5 3,624,215 + yhhJ b3485 0.46 +1.5 3,624,235 + yhhJ b3485 0.47 +0.2 3,624,238 - yhhJ b3485 0.48 -0.3 3,624,238 - yhhJ b3485 0.48 +0.4 3,624,238 - yhhJ b3485 0.48 +0.7 3,624,256 - yhhJ b3485 0.49 +0.9 3,624,256 - yhhJ b3485 0.49 -0.1 3,624,273 - yhhJ b3485 0.51 +1.0 3,624,317 - yhhJ b3485 0.55 +0.4 3,624,317 - yhhJ b3485 0.55 +0.4 3,624,365 + yhhJ b3485 0.59 +0.0 3,624,407 + yhhJ b3485 0.63 +0.7 3,624,407 + yhhJ b3485 0.63 +0.6 3,624,448 - yhhJ b3485 0.66 +0.4 3,624,489 + yhhJ b3485 0.70 -3.7
Or see this region's nucleotide sequence