Strain Fitness in Escherichia coli BW25113 around b2948

Experiment: carbon source D-Mannose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgshB and yqgE are separated by 108 nucleotidesyqgE and yqgF overlap by 1 nucleotidesyqgF and yggR are separated by 183 nucleotides b2947: gshB - glutathione synthetase (NCBI), at 3,089,900 to 3,090,850 gshB b2948: yqgE - orf, hypothetical protein (VIMSS), at 3,090,959 to 3,091,522 yqgE b2949: yqgF - Holliday junction resolvase-like protein (NCBI), at 3,091,522 to 3,091,938 yqgF b2950: yggR - putative protein transport (VIMSS), at 3,092,122 to 3,093,102 yggR Position (kb) 3090 3091 3092Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 3090.004 kb on + strand, within gshBat 3090.085 kb on - strand, within gshBat 3090.085 kb on - strand, within gshBat 3090.092 kb on - strand, within gshBat 3090.222 kb on + strand, within gshBat 3090.458 kb on - strand, within gshBat 3090.479 kb on - strand, within gshBat 3090.479 kb on - strand, within gshBat 3090.486 kb on - strand, within gshBat 3090.562 kb on - strand, within gshBat 3090.562 kb on - strand, within gshBat 3090.576 kb on + strand, within gshBat 3090.811 kb on + strandat 3090.847 kb on - strandat 3090.847 kb on - strandat 3090.883 kb on + strandat 3090.883 kb on + strandat 3090.893 kb on - strandat 3090.893 kb on - strandat 3091.078 kb on - strand, within yqgEat 3091.078 kb on - strand, within yqgEat 3091.140 kb on + strand, within yqgEat 3091.140 kb on + strand, within yqgEat 3091.271 kb on - strand, within yqgEat 3091.271 kb on - strand, within yqgEat 3091.328 kb on + strand, within yqgEat 3091.534 kb on + strandat 3091.929 kb on + strandat 3091.942 kb on + strandat 3091.973 kb on - strandat 3091.973 kb on - strandat 3091.982 kb on - strandat 3092.008 kb on - strandat 3092.032 kb on + strandat 3092.032 kb on + strandat 3092.034 kb on + strandat 3092.053 kb on + strandat 3092.156 kb on - strandat 3092.156 kb on - strandat 3092.336 kb on - strand, within yggRat 3092.338 kb on + strand, within yggRat 3092.338 kb on + strand, within yggRat 3092.357 kb on - strand, within yggRat 3092.395 kb on + strand, within yggRat 3092.398 kb on + strand, within yggR

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 20mM
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3,090,004 + gshB b2947 0.11 +1.2
3,090,085 - gshB b2947 0.19 +1.4
3,090,085 - gshB b2947 0.19 +0.4
3,090,092 - gshB b2947 0.20 -0.3
3,090,222 + gshB b2947 0.34 -2.6
3,090,458 - gshB b2947 0.59 +1.1
3,090,479 - gshB b2947 0.61 +0.5
3,090,479 - gshB b2947 0.61 +1.3
3,090,486 - gshB b2947 0.62 +1.0
3,090,562 - gshB b2947 0.70 -0.5
3,090,562 - gshB b2947 0.70 +0.3
3,090,576 + gshB b2947 0.71 +1.3
3,090,811 + +0.5
3,090,847 - +0.8
3,090,847 - +1.0
3,090,883 + -0.8
3,090,883 + -0.8
3,090,893 - +1.2
3,090,893 - -0.8
3,091,078 - yqgE b2948 0.21 +1.7
3,091,078 - yqgE b2948 0.21 +0.4
3,091,140 + yqgE b2948 0.32 +0.5
3,091,140 + yqgE b2948 0.32 -2.2
3,091,271 - yqgE b2948 0.55 -2.0
3,091,271 - yqgE b2948 0.55 +5.4
3,091,328 + yqgE b2948 0.65 +0.6
3,091,534 + -0.0
3,091,929 + -1.0
3,091,942 + +0.5
3,091,973 - -0.3
3,091,973 - +0.8
3,091,982 - -0.8
3,092,008 - +1.2
3,092,032 + -0.0
3,092,032 + -0.3
3,092,034 + -0.4
3,092,053 + -0.0
3,092,156 - +0.6
3,092,156 - -0.3
3,092,336 - yggR b2950 0.22 -1.6
3,092,338 + yggR b2950 0.22 -0.4
3,092,338 + yggR b2950 0.22 -2.8
3,092,357 - yggR b2950 0.24 +1.0
3,092,395 + yggR b2950 0.28 -0.1
3,092,398 + yggR b2950 0.28 -0.4

Or see this region's nucleotide sequence