Strain Fitness in Escherichia coli BW25113 around b2620

Experiment: carbon source D-Mannose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsmpA and b2618 are separated by 61 nucleotidesb2618 and yfjG overlap by 11 nucleotidesyfjG and smpB are separated by 131 nucleotidessmpB and ssrA are separated by 214 nucleotidesssrA and intA are separated by 203 nucleotides b2617: smpA - small membrane protein A (VIMSS), at 2,751,627 to 2,751,968 smpA b2618: b2618 - orf, hypothetical protein (VIMSS), at 2,752,030 to 2,752,320 b2618 b2619: yfjG - hypothetical protein (NCBI), at 2,752,310 to 2,752,786 yfjG b2620: smpB - SsrA-binding protein (NCBI), at 2,752,918 to 2,753,400 smpB b2621: ssrA - 10Sa RNA SsrA (NCBI), at 2,753,615 to 2,753,977 ssrA b2622: intA - CP4-57 prophage; integrase (NCBI), at 2,754,181 to 2,755,422 intA Position (kb) 2752 2753 2754Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2751.941 kb on + strandat 2752.081 kb on + strand, within b2618at 2752.081 kb on + strand, within b2618at 2752.092 kb on + strand, within b2618at 2752.118 kb on - strand, within b2618at 2752.150 kb on + strand, within b2618at 2752.822 kb on + strandat 2752.851 kb on - strandat 2752.936 kb on + strandat 2753.096 kb on + strand, within smpBat 2753.223 kb on - strand, within smpBat 2753.223 kb on - strand, within smpBat 2753.403 kb on + strandat 2753.530 kb on + strandat 2754.108 kb on + strandat 2754.230 kb on + strandat 2754.297 kb on + strandat 2754.297 kb on + strandat 2754.367 kb on - strand, within intA

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 20mM
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2,751,941 + +0.2
2,752,081 + b2618 b2618 0.18 +0.5
2,752,081 + b2618 b2618 0.18 +1.3
2,752,092 + b2618 b2618 0.21 -3.1
2,752,118 - b2618 b2618 0.30 -0.1
2,752,150 + b2618 b2618 0.41 -1.4
2,752,822 + -0.3
2,752,851 - -0.0
2,752,936 + -1.3
2,753,096 + smpB b2620 0.37 +1.6
2,753,223 - smpB b2620 0.63 -2.9
2,753,223 - smpB b2620 0.63 +1.1
2,753,403 + -0.1
2,753,530 + -3.2
2,754,108 + +1.8
2,754,230 + +1.1
2,754,297 + -0.3
2,754,297 + +1.8
2,754,367 - intA b2622 0.15 -0.4

Or see this region's nucleotide sequence