Experiment: carbon source D-Mannose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt napD and napF overlap by 11 nucleotides napF and yojO are separated by 108 nucleotides yojO and eco are separated by 134 nucleotides eco and mqo are separated by 714 nucleotides
b2207: napD - assembly protein for periplasmic nitrate reductase (NCBI), at 2,300,772 to 2,301,035
napD
b2208: napF - ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI), at 2,301,025 to 2,301,519
napF
b4604: yojO - hypothetical protein (RefSeq), at 2,301,628 to 2,301,792
yojO
b2209: eco - ecotin precursor (NCBI), at 2,301,927 to 2,302,415
eco
b2210: mqo - malate:quinone oxidoreductase (NCBI), at 2,303,130 to 2,304,776
mqo
Position (kb)
2301
2302
2303 Strain fitness (log2 ratio)
-2
-1
0
1 at 2300.965 kb on + strand, within napD at 2301.037 kb on + strand at 2301.037 kb on + strand at 2301.060 kb on + strand at 2301.060 kb on + strand at 2301.084 kb on + strand, within napF at 2301.183 kb on - strand, within napF at 2301.210 kb on - strand, within napF at 2301.210 kb on - strand, within napF at 2301.256 kb on - strand, within napF at 2301.257 kb on - strand, within napF at 2301.286 kb on + strand, within napF at 2301.415 kb on - strand, within napF at 2301.496 kb on - strand at 2301.620 kb on + strand at 2301.874 kb on + strand at 2302.083 kb on + strand, within eco at 2302.294 kb on + strand, within eco at 2302.310 kb on - strand, within eco at 2302.522 kb on + strand at 2302.748 kb on + strand at 2302.763 kb on + strand at 2302.763 kb on + strand at 2302.773 kb on + strand at 2303.223 kb on - strand at 2303.225 kb on + strand at 2303.226 kb on + strand at 2303.244 kb on + strand at 2303.346 kb on - strand, within mqo at 2303.346 kb on - strand, within mqo
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Mannose 20mM remove 2,300,965 + napD b2207 0.73 -0.9 2,301,037 + +0.5 2,301,037 + -0.3 2,301,060 + +0.2 2,301,060 + +0.1 2,301,084 + napF b2208 0.12 +0.0 2,301,183 - napF b2208 0.32 -1.1 2,301,210 - napF b2208 0.37 -1.5 2,301,210 - napF b2208 0.37 -1.8 2,301,256 - napF b2208 0.47 -0.4 2,301,257 - napF b2208 0.47 -0.3 2,301,286 + napF b2208 0.53 -0.5 2,301,415 - napF b2208 0.79 +0.4 2,301,496 - +0.9 2,301,620 + +1.0 2,301,874 + -1.1 2,302,083 + eco b2209 0.32 +0.4 2,302,294 + eco b2209 0.75 -1.5 2,302,310 - eco b2209 0.78 +1.4 2,302,522 + +0.2 2,302,748 + -0.1 2,302,763 + -1.9 2,302,763 + +0.4 2,302,773 + +0.3 2,303,223 - +0.3 2,303,225 + -1.0 2,303,226 + -0.3 2,303,244 + -1.5 2,303,346 - mqo b2210 0.13 +0.6 2,303,346 - mqo b2210 0.13 -0.1
Or see this region's nucleotide sequence