Strain Fitness in Escherichia coli BW25113 around b0092

Experiment: carbon source D-Mannose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmurC and ddlB overlap by 8 nucleotidesddlB and ftsQ are separated by 1 nucleotidesftsQ and ftsA overlap by 4 nucleotides b0091: murC - UDP-N-acetylmuramate--L-alanine ligase (NCBI), at 100,765 to 102,240 murC b0092: ddlB - D-alanylalanine synthetase (NCBI), at 102,233 to 103,153 ddlB b0093: ftsQ - membrane anchored protein involved in growth of wall at septum (NCBI), at 103,155 to 103,985 ftsQ b0094: ftsA - cell division protein (NCBI), at 103,982 to 105,244 ftsA Position (kb) 102 103 104Strain fitness (log2 ratio) -1 0 1 2 3at 102.254 kb on + strandat 102.265 kb on + strandat 102.265 kb on + strandat 102.295 kb on + strandat 102.446 kb on + strand, within ddlBat 102.492 kb on + strand, within ddlBat 102.492 kb on + strand, within ddlBat 102.496 kb on + strand, within ddlBat 102.498 kb on + strand, within ddlBat 102.573 kb on + strand, within ddlBat 102.573 kb on + strand, within ddlBat 102.573 kb on + strand, within ddlBat 102.793 kb on + strand, within ddlBat 102.829 kb on + strand, within ddlBat 102.984 kb on + strand, within ddlBat 103.002 kb on + strand, within ddlBat 103.017 kb on + strand, within ddlBat 103.068 kb on + strandat 103.138 kb on + strandat 103.911 kb on + strandat 103.911 kb on + strandat 103.916 kb on + strandat 103.916 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 20mM
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102,254 + -0.6
102,265 + +0.4
102,265 + +1.7
102,295 + +1.8
102,446 + ddlB b0092 0.23 +0.9
102,492 + ddlB b0092 0.28 +1.0
102,492 + ddlB b0092 0.28 +1.9
102,496 + ddlB b0092 0.29 +1.2
102,498 + ddlB b0092 0.29 +1.1
102,573 + ddlB b0092 0.37 +1.5
102,573 + ddlB b0092 0.37 +2.0
102,573 + ddlB b0092 0.37 +1.4
102,793 + ddlB b0092 0.61 +1.8
102,829 + ddlB b0092 0.65 +1.7
102,984 + ddlB b0092 0.82 +0.1
103,002 + ddlB b0092 0.83 +0.6
103,017 + ddlB b0092 0.85 +1.7
103,068 + +1.1
103,138 + +2.8
103,911 + -0.3
103,911 + -0.3
103,916 + -0.1
103,916 + +0.8

Or see this region's nucleotide sequence