Experiment: carbon source D-Glucose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ffh and ypjD are separated by 166 nucleotides ypjD and yfjD are separated by 20 nucleotides yfjD and grpE are separated by 54 nucleotides grpE and yfjB are separated by 122 nucleotides
b2610: ffh - Signal Recognition Particle (SRP) component with 4.5S RNA (ffs) (NCBI), at 2,744,456 to 2,745,817
ffh
b2611: ypjD - predicted inner membrane protein (RefSeq), at 2,745,984 to 2,746,775
ypjD
b4461: yfjD - predicted inner membrane protein (RefSeq), at 2,746,796 to 2,748,082
yfjD
b2614: grpE - heat shock protein (NCBI), at 2,748,137 to 2,748,730
grpE
b2615: yfjB - inorganic polyphosphate/ATP-NAD kinase (NCBI), at 2,748,853 to 2,749,731
yfjB
Position (kb)
2746
2747
2748
2749 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2745.896 kb on - strand at 2745.896 kb on - strand at 2745.945 kb on - strand at 2745.945 kb on - strand at 2746.143 kb on - strand, within ypjD at 2746.191 kb on + strand, within ypjD at 2746.221 kb on - strand, within ypjD at 2746.257 kb on + strand, within ypjD at 2746.257 kb on + strand, within ypjD at 2746.257 kb on + strand, within ypjD at 2746.269 kb on + strand, within ypjD at 2746.269 kb on + strand, within ypjD at 2746.269 kb on + strand, within ypjD at 2746.277 kb on - strand, within ypjD at 2746.290 kb on + strand, within ypjD at 2746.301 kb on + strand, within ypjD at 2746.363 kb on + strand, within ypjD at 2746.425 kb on - strand, within ypjD at 2746.435 kb on - strand, within ypjD at 2746.503 kb on - strand, within ypjD at 2746.563 kb on + strand, within ypjD at 2746.563 kb on + strand, within ypjD at 2746.609 kb on + strand, within ypjD at 2746.627 kb on - strand, within ypjD at 2746.627 kb on - strand, within ypjD at 2746.645 kb on - strand, within ypjD at 2746.645 kb on - strand, within ypjD at 2746.763 kb on - strand at 2746.804 kb on + strand at 2746.817 kb on + strand at 2746.817 kb on + strand at 2746.820 kb on - strand at 2746.957 kb on + strand, within yfjD at 2746.981 kb on + strand, within yfjD at 2746.985 kb on - strand, within yfjD at 2747.009 kb on - strand, within yfjD at 2747.018 kb on + strand, within yfjD at 2747.152 kb on + strand, within yfjD at 2747.315 kb on - strand, within yfjD at 2747.315 kb on - strand, within yfjD at 2747.324 kb on + strand, within yfjD at 2747.332 kb on - strand, within yfjD at 2747.414 kb on - strand, within yfjD at 2747.414 kb on - strand, within yfjD at 2747.503 kb on - strand, within yfjD at 2747.503 kb on - strand, within yfjD at 2747.554 kb on + strand, within yfjD at 2747.647 kb on - strand, within yfjD at 2747.647 kb on - strand, within yfjD at 2747.654 kb on - strand, within yfjD at 2747.717 kb on - strand, within yfjD at 2747.740 kb on + strand, within yfjD at 2747.765 kb on - strand, within yfjD at 2747.796 kb on + strand, within yfjD at 2747.805 kb on - strand, within yfjD at 2747.807 kb on + strand, within yfjD at 2747.822 kb on + strand, within yfjD at 2747.955 kb on + strand at 2747.984 kb on + strand at 2748.001 kb on - strand at 2748.011 kb on + strand at 2748.025 kb on + strand at 2748.069 kb on - strand at 2748.571 kb on - strand, within grpE at 2748.623 kb on - strand, within grpE at 2748.804 kb on + strand at 2748.810 kb on + strand at 2748.818 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 20mM remove 2,745,896 - +2.1 2,745,896 - +0.1 2,745,945 - -0.2 2,745,945 - +0.5 2,746,143 - ypjD b2611 0.20 -0.2 2,746,191 + ypjD b2611 0.26 +1.6 2,746,221 - ypjD b2611 0.30 +0.7 2,746,257 + ypjD b2611 0.34 -1.0 2,746,257 + ypjD b2611 0.34 -0.4 2,746,257 + ypjD b2611 0.34 -2.3 2,746,269 + ypjD b2611 0.36 +0.3 2,746,269 + ypjD b2611 0.36 -1.7 2,746,269 + ypjD b2611 0.36 +1.3 2,746,277 - ypjD b2611 0.37 -0.2 2,746,290 + ypjD b2611 0.39 +0.0 2,746,301 + ypjD b2611 0.40 -2.5 2,746,363 + ypjD b2611 0.48 -1.3 2,746,425 - ypjD b2611 0.56 +0.8 2,746,435 - ypjD b2611 0.57 -0.1 2,746,503 - ypjD b2611 0.66 +0.1 2,746,563 + ypjD b2611 0.73 -1.1 2,746,563 + ypjD b2611 0.73 +1.1 2,746,609 + ypjD b2611 0.79 -2.5 2,746,627 - ypjD b2611 0.81 -0.1 2,746,627 - ypjD b2611 0.81 +0.5 2,746,645 - ypjD b2611 0.83 +0.8 2,746,645 - ypjD b2611 0.83 +1.1 2,746,763 - +1.1 2,746,804 + +0.3 2,746,817 + +0.1 2,746,817 + +0.7 2,746,820 - -2.6 2,746,957 + yfjD b4461 0.13 +1.0 2,746,981 + yfjD b4461 0.14 +0.7 2,746,985 - yfjD b4461 0.15 +1.7 2,747,009 - yfjD b4461 0.17 +0.9 2,747,018 + yfjD b4461 0.17 +1.1 2,747,152 + yfjD b4461 0.28 +0.1 2,747,315 - yfjD b4461 0.40 -0.2 2,747,315 - yfjD b4461 0.40 +0.4 2,747,324 + yfjD b4461 0.41 +0.6 2,747,332 - yfjD b4461 0.42 +1.0 2,747,414 - yfjD b4461 0.48 +1.5 2,747,414 - yfjD b4461 0.48 +0.5 2,747,503 - yfjD b4461 0.55 +1.7 2,747,503 - yfjD b4461 0.55 -2.2 2,747,554 + yfjD b4461 0.59 +1.0 2,747,647 - yfjD b4461 0.66 -2.8 2,747,647 - yfjD b4461 0.66 +0.5 2,747,654 - yfjD b4461 0.67 +0.1 2,747,717 - yfjD b4461 0.72 -2.6 2,747,740 + yfjD b4461 0.73 -0.2 2,747,765 - yfjD b4461 0.75 +0.4 2,747,796 + yfjD b4461 0.78 +1.7 2,747,805 - yfjD b4461 0.78 +0.9 2,747,807 + yfjD b4461 0.79 +1.0 2,747,822 + yfjD b4461 0.80 +1.6 2,747,955 + -0.0 2,747,984 + +1.4 2,748,001 - -0.2 2,748,011 + +1.0 2,748,025 + +0.3 2,748,069 - +0.7 2,748,571 - grpE b2614 0.73 +3.3 2,748,623 - grpE b2614 0.82 -0.1 2,748,804 + +0.5 2,748,810 + +1.0 2,748,818 - -1.3
Or see this region's nucleotide sequence