Experiment: carbon source D-Glucose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt slyX and slyD are separated by 48 nucleotides slyD and yheV are separated by 94 nucleotides yheV and kefB are separated by 9 nucleotides kefB and kefG overlap by 1 nucleotides kefG and yheS are separated by 127 nucleotides
b3348: slyX - hypothetical protein (NCBI), at 3,475,662 to 3,475,880
slyX
b3349: slyD - FKBP-type peptidyl prolyl cis-trans isomerase (rotamase) (NCBI), at 3,475,929 to 3,476,519
slyD
b4551: yheV - hypothetical protein (NCBI), at 3,476,614 to 3,476,814
yheV
b3350: kefB - glutathione-regulated potassium-efflux system protein (NCBI), at 3,476,824 to 3,478,629
kefB
b3351: kefG - glutathione-regulated potassium-efflux system ancillary protein (NCBI), at 3,478,629 to 3,479,183
kefG
b3352: yheS - fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 3,479,311 to 3,481,224
yheS
Position (kb)
3476
3477
3478
3479 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3475.861 kb on + strand at 3475.874 kb on + strand at 3475.874 kb on + strand at 3475.939 kb on - strand at 3475.971 kb on + strand at 3475.991 kb on - strand, within slyD at 3475.991 kb on - strand, within slyD at 3476.000 kb on - strand, within slyD at 3476.042 kb on + strand at 3476.042 kb on + strand, within slyD at 3476.061 kb on + strand, within slyD at 3476.061 kb on + strand, within slyD at 3476.063 kb on + strand, within slyD at 3476.063 kb on + strand, within slyD at 3476.089 kb on - strand, within slyD at 3476.089 kb on - strand, within slyD at 3476.114 kb on - strand, within slyD at 3476.143 kb on + strand, within slyD at 3476.206 kb on + strand, within slyD at 3476.212 kb on - strand, within slyD at 3476.242 kb on + strand, within slyD at 3476.286 kb on + strand, within slyD at 3476.333 kb on - strand, within slyD at 3476.378 kb on - strand, within slyD at 3476.423 kb on - strand, within slyD at 3476.423 kb on - strand, within slyD at 3476.465 kb on + strand at 3476.473 kb on - strand at 3476.473 kb on - strand at 3476.479 kb on + strand at 3476.479 kb on + strand at 3476.485 kb on - strand at 3476.485 kb on - strand at 3476.522 kb on - strand at 3476.525 kb on + strand at 3476.525 kb on + strand at 3476.525 kb on + strand at 3476.525 kb on + strand at 3476.544 kb on - strand at 3476.544 kb on - strand at 3476.549 kb on - strand at 3476.612 kb on - strand at 3476.649 kb on - strand, within yheV at 3476.768 kb on - strand, within yheV at 3476.810 kb on + strand at 3476.853 kb on - strand at 3476.917 kb on - strand at 3476.917 kb on - strand at 3476.933 kb on + strand at 3476.941 kb on - strand at 3476.941 kb on - strand at 3476.941 kb on - strand at 3476.972 kb on - strand at 3477.015 kb on - strand, within kefB at 3477.021 kb on - strand at 3477.047 kb on - strand, within kefB at 3477.096 kb on + strand, within kefB at 3477.096 kb on + strand, within kefB at 3477.101 kb on + strand, within kefB at 3477.101 kb on + strand, within kefB at 3477.109 kb on - strand, within kefB at 3477.109 kb on - strand, within kefB at 3477.111 kb on + strand, within kefB at 3477.111 kb on + strand, within kefB at 3477.113 kb on - strand, within kefB at 3477.113 kb on - strand, within kefB at 3477.129 kb on - strand at 3477.129 kb on - strand, within kefB at 3477.131 kb on + strand, within kefB at 3477.131 kb on - strand, within kefB at 3477.131 kb on - strand, within kefB at 3477.164 kb on - strand, within kefB at 3477.164 kb on - strand, within kefB at 3477.189 kb on + strand, within kefB at 3477.189 kb on + strand, within kefB at 3477.197 kb on - strand, within kefB at 3477.197 kb on - strand, within kefB at 3477.201 kb on + strand, within kefB at 3477.218 kb on - strand, within kefB at 3477.219 kb on - strand, within kefB at 3477.256 kb on + strand, within kefB at 3477.256 kb on + strand, within kefB at 3477.271 kb on - strand, within kefB at 3477.271 kb on - strand, within kefB at 3477.313 kb on - strand, within kefB at 3477.313 kb on - strand, within kefB at 3477.326 kb on + strand, within kefB at 3477.337 kb on - strand, within kefB at 3477.337 kb on - strand, within kefB at 3477.350 kb on - strand, within kefB at 3477.388 kb on + strand, within kefB at 3477.417 kb on - strand, within kefB at 3477.506 kb on + strand, within kefB at 3477.515 kb on - strand, within kefB at 3477.574 kb on + strand, within kefB at 3477.579 kb on + strand, within kefB at 3477.617 kb on + strand, within kefB at 3477.617 kb on + strand, within kefB at 3477.634 kb on - strand, within kefB at 3477.636 kb on + strand, within kefB at 3477.668 kb on + strand, within kefB at 3477.678 kb on + strand, within kefB at 3477.688 kb on - strand, within kefB at 3477.724 kb on + strand, within kefB at 3477.727 kb on + strand, within kefB at 3477.735 kb on - strand, within kefB at 3477.752 kb on - strand, within kefB at 3477.757 kb on - strand, within kefB at 3477.757 kb on - strand, within kefB at 3477.757 kb on - strand, within kefB at 3477.762 kb on - strand, within kefB at 3477.764 kb on + strand, within kefB at 3477.764 kb on + strand, within kefB at 3477.764 kb on + strand, within kefB at 3477.772 kb on - strand, within kefB at 3477.772 kb on - strand, within kefB at 3477.772 kb on - strand, within kefB at 3477.772 kb on - strand, within kefB at 3477.827 kb on + strand, within kefB at 3477.827 kb on + strand, within kefB at 3477.827 kb on + strand, within kefB at 3477.835 kb on + strand, within kefB at 3477.863 kb on - strand, within kefB at 3477.863 kb on - strand, within kefB at 3477.921 kb on + strand, within kefB at 3477.956 kb on + strand, within kefB at 3477.982 kb on + strand, within kefB at 3477.982 kb on + strand, within kefB at 3477.993 kb on + strand, within kefB at 3478.001 kb on - strand, within kefB at 3478.001 kb on - strand, within kefB at 3478.001 kb on - strand, within kefB at 3478.062 kb on - strand, within kefB at 3478.062 kb on - strand, within kefB at 3478.071 kb on + strand, within kefB at 3478.079 kb on - strand, within kefB at 3478.082 kb on + strand, within kefB at 3478.099 kb on - strand, within kefB at 3478.118 kb on + strand, within kefB at 3478.130 kb on + strand, within kefB at 3478.135 kb on + strand, within kefB at 3478.135 kb on + strand, within kefB at 3478.138 kb on - strand, within kefB at 3478.223 kb on + strand, within kefB at 3478.223 kb on + strand, within kefB at 3478.247 kb on + strand, within kefB at 3478.255 kb on - strand, within kefB at 3478.255 kb on - strand, within kefB at 3478.272 kb on - strand, within kefB at 3478.279 kb on - strand, within kefB at 3478.279 kb on - strand, within kefB at 3478.291 kb on - strand, within kefB at 3478.291 kb on - strand, within kefB at 3478.408 kb on - strand, within kefB at 3478.427 kb on - strand, within kefB at 3478.440 kb on - strand, within kefB at 3478.470 kb on - strand at 3478.494 kb on - strand at 3478.528 kb on + strand at 3478.547 kb on - strand at 3478.576 kb on + strand at 3478.578 kb on - strand at 3478.578 kb on - strand at 3478.637 kb on + strand at 3478.639 kb on + strand at 3478.639 kb on + strand at 3478.669 kb on + strand at 3478.680 kb on + strand at 3478.694 kb on + strand, within kefG at 3478.694 kb on + strand, within kefG at 3478.736 kb on + strand, within kefG at 3478.755 kb on + strand, within kefG at 3478.755 kb on + strand, within kefG at 3478.780 kb on + strand, within kefG at 3478.780 kb on + strand, within kefG at 3478.780 kb on - strand, within kefG at 3478.780 kb on - strand, within kefG at 3478.794 kb on + strand, within kefG at 3478.794 kb on + strand, within kefG at 3478.819 kb on + strand, within kefG at 3478.845 kb on + strand, within kefG at 3478.869 kb on - strand, within kefG at 3478.937 kb on - strand, within kefG at 3478.941 kb on + strand, within kefG at 3478.944 kb on + strand, within kefG at 3478.965 kb on + strand, within kefG at 3478.965 kb on + strand, within kefG at 3478.966 kb on + strand, within kefG at 3478.966 kb on + strand, within kefG at 3478.988 kb on + strand, within kefG at 3478.988 kb on + strand, within kefG at 3479.017 kb on - strand, within kefG at 3479.017 kb on - strand, within kefG at 3479.057 kb on + strand, within kefG at 3479.057 kb on + strand, within kefG at 3479.080 kb on + strand, within kefG at 3479.085 kb on + strand, within kefG at 3479.088 kb on - strand, within kefG at 3479.098 kb on + strand, within kefG at 3479.108 kb on + strand, within kefG at 3479.154 kb on - strand at 3479.170 kb on + strand at 3479.178 kb on - strand at 3479.178 kb on - strand at 3479.205 kb on + strand at 3479.205 kb on + strand at 3479.205 kb on + strand at 3479.305 kb on - strand at 3479.305 kb on - strand at 3479.437 kb on - strand at 3479.437 kb on - strand at 3479.445 kb on - strand at 3479.445 kb on - strand at 3479.486 kb on + strand at 3479.599 kb on + strand, within yheS at 3479.607 kb on - strand, within yheS at 3479.607 kb on - strand, within yheS at 3479.628 kb on - strand, within yheS
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 20mM remove 3,475,861 + +0.3 3,475,874 + +1.5 3,475,874 + -0.3 3,475,939 - +0.6 3,475,971 + +0.9 3,475,991 - slyD b3349 0.10 +0.1 3,475,991 - slyD b3349 0.10 +2.1 3,476,000 - slyD b3349 0.12 +0.5 3,476,042 + +1.2 3,476,042 + slyD b3349 0.19 +2.3 3,476,061 + slyD b3349 0.22 -2.1 3,476,061 + slyD b3349 0.22 -2.9 3,476,063 + slyD b3349 0.23 -0.5 3,476,063 + slyD b3349 0.23 -2.2 3,476,089 - slyD b3349 0.27 +0.3 3,476,089 - slyD b3349 0.27 +0.3 3,476,114 - slyD b3349 0.31 -0.5 3,476,143 + slyD b3349 0.36 +1.4 3,476,206 + slyD b3349 0.47 +0.0 3,476,212 - slyD b3349 0.48 -0.1 3,476,242 + slyD b3349 0.53 -1.5 3,476,286 + slyD b3349 0.60 -0.0 3,476,333 - slyD b3349 0.68 +0.0 3,476,378 - slyD b3349 0.76 +0.3 3,476,423 - slyD b3349 0.84 +0.4 3,476,423 - slyD b3349 0.84 -1.1 3,476,465 + +0.6 3,476,473 - +0.4 3,476,473 - +1.0 3,476,479 + +1.7 3,476,479 + +0.9 3,476,485 - -0.1 3,476,485 - -0.4 3,476,522 - -0.6 3,476,525 + +0.7 3,476,525 + +1.1 3,476,525 + +0.1 3,476,525 + -1.3 3,476,544 - +0.1 3,476,544 - -0.2 3,476,549 - +0.0 3,476,612 - -0.6 3,476,649 - yheV b4551 0.17 +0.3 3,476,768 - yheV b4551 0.77 +1.0 3,476,810 + +2.0 3,476,853 - -1.6 3,476,917 - +0.8 3,476,917 - +0.7 3,476,933 + +1.9 3,476,941 - +0.9 3,476,941 - -0.1 3,476,941 - -0.7 3,476,972 - +1.4 3,477,015 - kefB b3350 0.11 +0.5 3,477,021 - -1.2 3,477,047 - kefB b3350 0.12 +0.4 3,477,096 + kefB b3350 0.15 +0.9 3,477,096 + kefB b3350 0.15 +1.1 3,477,101 + kefB b3350 0.15 +1.0 3,477,101 + kefB b3350 0.15 +0.5 3,477,109 - kefB b3350 0.16 +0.6 3,477,109 - kefB b3350 0.16 +2.0 3,477,111 + kefB b3350 0.16 +1.5 3,477,111 + kefB b3350 0.16 +1.2 3,477,113 - kefB b3350 0.16 +0.3 3,477,113 - kefB b3350 0.16 +1.0 3,477,129 - +1.6 3,477,129 - kefB b3350 0.17 -0.4 3,477,131 + kefB b3350 0.17 -0.5 3,477,131 - kefB b3350 0.17 -1.0 3,477,131 - kefB b3350 0.17 +1.9 3,477,164 - kefB b3350 0.19 +1.5 3,477,164 - kefB b3350 0.19 -1.9 3,477,189 + kefB b3350 0.20 +1.2 3,477,189 + kefB b3350 0.20 -0.9 3,477,197 - kefB b3350 0.21 +0.8 3,477,197 - kefB b3350 0.21 +0.6 3,477,201 + kefB b3350 0.21 +0.1 3,477,218 - kefB b3350 0.22 +0.5 3,477,219 - kefB b3350 0.22 +0.6 3,477,256 + kefB b3350 0.24 -0.5 3,477,256 + kefB b3350 0.24 +0.9 3,477,271 - kefB b3350 0.25 +1.6 3,477,271 - kefB b3350 0.25 +1.4 3,477,313 - kefB b3350 0.27 +0.4 3,477,313 - kefB b3350 0.27 +1.0 3,477,326 + kefB b3350 0.28 -0.6 3,477,337 - kefB b3350 0.28 +0.3 3,477,337 - kefB b3350 0.28 +0.8 3,477,350 - kefB b3350 0.29 +0.8 3,477,388 + kefB b3350 0.31 +1.8 3,477,417 - kefB b3350 0.33 -0.4 3,477,506 + kefB b3350 0.38 +0.2 3,477,515 - kefB b3350 0.38 +0.8 3,477,574 + kefB b3350 0.42 +0.3 3,477,579 + kefB b3350 0.42 +0.2 3,477,617 + kefB b3350 0.44 +0.8 3,477,617 + kefB b3350 0.44 -1.7 3,477,634 - kefB b3350 0.45 +1.4 3,477,636 + kefB b3350 0.45 +1.0 3,477,668 + kefB b3350 0.47 +0.6 3,477,678 + kefB b3350 0.47 +1.2 3,477,688 - kefB b3350 0.48 -0.4 3,477,724 + kefB b3350 0.50 -0.2 3,477,727 + kefB b3350 0.50 -0.3 3,477,735 - kefB b3350 0.50 +1.0 3,477,752 - kefB b3350 0.51 +0.6 3,477,757 - kefB b3350 0.52 +0.6 3,477,757 - kefB b3350 0.52 +1.4 3,477,757 - kefB b3350 0.52 -2.5 3,477,762 - kefB b3350 0.52 +0.7 3,477,764 + kefB b3350 0.52 +1.3 3,477,764 + kefB b3350 0.52 +0.7 3,477,764 + kefB b3350 0.52 +1.0 3,477,772 - kefB b3350 0.52 +0.4 3,477,772 - kefB b3350 0.52 +1.4 3,477,772 - kefB b3350 0.52 +0.6 3,477,772 - kefB b3350 0.52 +0.8 3,477,827 + kefB b3350 0.56 +1.0 3,477,827 + kefB b3350 0.56 +2.6 3,477,827 + kefB b3350 0.56 -2.9 3,477,835 + kefB b3350 0.56 -0.1 3,477,863 - kefB b3350 0.58 +1.5 3,477,863 - kefB b3350 0.58 +0.6 3,477,921 + kefB b3350 0.61 +1.2 3,477,956 + kefB b3350 0.63 +1.0 3,477,982 + kefB b3350 0.64 +0.6 3,477,982 + kefB b3350 0.64 -1.5 3,477,993 + kefB b3350 0.65 -0.6 3,478,001 - kefB b3350 0.65 +0.7 3,478,001 - kefB b3350 0.65 +1.1 3,478,001 - kefB b3350 0.65 +1.6 3,478,062 - kefB b3350 0.69 +0.8 3,478,062 - kefB b3350 0.69 +1.7 3,478,071 + kefB b3350 0.69 +0.3 3,478,079 - kefB b3350 0.69 +0.6 3,478,082 + kefB b3350 0.70 +1.2 3,478,099 - kefB b3350 0.71 +1.2 3,478,118 + kefB b3350 0.72 +1.3 3,478,130 + kefB b3350 0.72 +0.9 3,478,135 + kefB b3350 0.73 +0.5 3,478,135 + kefB b3350 0.73 +1.2 3,478,138 - kefB b3350 0.73 +1.0 3,478,223 + kefB b3350 0.77 +0.5 3,478,223 + kefB b3350 0.77 +0.3 3,478,247 + kefB b3350 0.79 -0.3 3,478,255 - kefB b3350 0.79 +0.8 3,478,255 - kefB b3350 0.79 +0.7 3,478,272 - kefB b3350 0.80 +0.5 3,478,279 - kefB b3350 0.81 +0.3 3,478,279 - kefB b3350 0.81 +0.3 3,478,291 - kefB b3350 0.81 +0.9 3,478,291 - kefB b3350 0.81 -0.5 3,478,408 - kefB b3350 0.88 +0.9 3,478,427 - kefB b3350 0.89 +0.5 3,478,440 - kefB b3350 0.89 -0.5 3,478,470 - +0.2 3,478,494 - +0.8 3,478,528 + +1.7 3,478,547 - +0.3 3,478,576 + +0.7 3,478,578 - +0.3 3,478,578 - +0.9 3,478,637 + +1.1 3,478,639 + +0.5 3,478,639 + +0.9 3,478,669 + +0.5 3,478,680 + +0.7 3,478,694 + kefG b3351 0.12 +0.2 3,478,694 + kefG b3351 0.12 +0.6 3,478,736 + kefG b3351 0.19 +1.2 3,478,755 + kefG b3351 0.23 +2.1 3,478,755 + kefG b3351 0.23 +0.8 3,478,780 + kefG b3351 0.27 +1.2 3,478,780 + kefG b3351 0.27 +0.5 3,478,780 - kefG b3351 0.27 +1.8 3,478,780 - kefG b3351 0.27 +0.9 3,478,794 + kefG b3351 0.30 +1.1 3,478,794 + kefG b3351 0.30 -0.1 3,478,819 + kefG b3351 0.34 +1.5 3,478,845 + kefG b3351 0.39 +1.4 3,478,869 - kefG b3351 0.43 +0.3 3,478,937 - kefG b3351 0.55 -0.2 3,478,941 + kefG b3351 0.56 +0.4 3,478,944 + kefG b3351 0.57 +0.4 3,478,965 + kefG b3351 0.61 +1.1 3,478,965 + kefG b3351 0.61 -0.2 3,478,966 + kefG b3351 0.61 -1.0 3,478,966 + kefG b3351 0.61 -2.5 3,478,988 + kefG b3351 0.65 -0.4 3,478,988 + kefG b3351 0.65 -0.3 3,479,017 - kefG b3351 0.70 +2.9 3,479,017 - kefG b3351 0.70 +3.0 3,479,057 + kefG b3351 0.77 +1.1 3,479,057 + kefG b3351 0.77 +0.9 3,479,080 + kefG b3351 0.81 +1.0 3,479,085 + kefG b3351 0.82 +0.8 3,479,088 - kefG b3351 0.83 +0.1 3,479,098 + kefG b3351 0.85 -0.6 3,479,108 + kefG b3351 0.86 +1.2 3,479,154 - +0.6 3,479,170 + +0.5 3,479,178 - -0.6 3,479,178 - -0.7 3,479,205 + +0.3 3,479,205 + +0.1 3,479,205 + +0.9 3,479,305 - +1.1 3,479,305 - -2.1 3,479,437 - -0.1 3,479,437 - -0.6 3,479,445 - -3.5 3,479,445 - -1.1 3,479,486 + +0.6 3,479,599 + yheS b3352 0.15 +0.4 3,479,607 - yheS b3352 0.15 -0.8 3,479,607 - yheS b3352 0.15 +0.4 3,479,628 - yheS b3352 0.17 +1.9
Or see this region's nucleotide sequence