Strain Fitness in Escherichia coli BW25113 around b3164

Experiment: carbon source D-Glucose 20mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntnlpI and pnp are separated by 108 nucleotidespnp and sraG are separated by 56 nucleotidessraG and rpsO are separated by 16 nucleotidesrpsO and truB are separated by 148 nucleotides b3163: nlpI - hypothetical protein (NCBI), at 3,306,062 to 3,306,946 nlpI b3164: pnp - polynucleotide phosphorylase/polyadenylase (RefSeq), at 3,307,055 to 3,309,190 pnp b4449: sraG - unknown RNA (NCBI), at 3,309,247 to 3,309,420 sraG b3165: rpsO - 30S ribosomal protein S15 (NCBI), at 3,309,437 to 3,309,706 rpsO b3166: truB - tRNA pseudouridine synthase B (NCBI), at 3,309,855 to 3,310,799 truB Position (kb) 3307 3308 3309 3310Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3306.073 kb on - strandat 3306.103 kb on - strandat 3306.171 kb on + strand, within nlpIat 3306.189 kb on - strand, within nlpIat 3306.189 kb on - strand, within nlpIat 3306.192 kb on - strand, within nlpIat 3306.216 kb on - strand, within nlpIat 3306.275 kb on - strand, within nlpIat 3306.275 kb on - strand, within nlpIat 3306.640 kb on - strand, within nlpIat 3306.640 kb on - strand, within nlpIat 3306.652 kb on - strand, within nlpIat 3306.676 kb on + strand, within nlpIat 3306.712 kb on + strand, within nlpIat 3306.728 kb on + strand, within nlpIat 3306.729 kb on + strand, within nlpIat 3306.855 kb on - strand, within nlpIat 3307.056 kb on + strandat 3307.056 kb on + strandat 3307.069 kb on - strandat 3307.069 kb on - strandat 3307.074 kb on - strandat 3307.095 kb on - strandat 3307.117 kb on + strandat 3307.200 kb on - strandat 3307.247 kb on - strandat 3307.279 kb on - strand, within pnpat 3307.314 kb on - strand, within pnpat 3307.354 kb on - strand, within pnpat 3307.359 kb on - strand, within pnpat 3307.371 kb on - strand, within pnpat 3307.471 kb on - strand, within pnpat 3309.216 kb on - strandat 3309.239 kb on - strandat 3309.437 kb on - strandat 3309.823 kb on - strandat 3309.823 kb on - strandat 3309.838 kb on - strandat 3309.987 kb on - strand, within truBat 3309.990 kb on - strand, within truBat 3310.065 kb on + strand, within truBat 3310.085 kb on - strand, within truBat 3310.126 kb on + strand, within truBat 3310.148 kb on + strand, within truBat 3310.148 kb on + strand, within truB

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 20mM
remove
3,306,073 - -0.4
3,306,103 - -1.9
3,306,171 + nlpI b3163 0.12 +0.5
3,306,189 - nlpI b3163 0.14 +2.3
3,306,189 - nlpI b3163 0.14 +2.2
3,306,192 - nlpI b3163 0.15 +1.0
3,306,216 - nlpI b3163 0.17 +2.1
3,306,275 - nlpI b3163 0.24 +1.4
3,306,275 - nlpI b3163 0.24 +2.3
3,306,640 - nlpI b3163 0.65 +4.6
3,306,640 - nlpI b3163 0.65 +2.1
3,306,652 - nlpI b3163 0.67 +2.8
3,306,676 + nlpI b3163 0.69 +1.3
3,306,712 + nlpI b3163 0.73 +1.0
3,306,728 + nlpI b3163 0.75 +0.7
3,306,729 + nlpI b3163 0.75 +0.9
3,306,855 - nlpI b3163 0.90 +2.0
3,307,056 + -0.6
3,307,056 + +0.7
3,307,069 - +1.6
3,307,069 - +1.0
3,307,074 - -0.4
3,307,095 - +0.4
3,307,117 + +1.5
3,307,200 - +2.4
3,307,247 - +1.4
3,307,279 - pnp b3164 0.10 +1.3
3,307,314 - pnp b3164 0.12 +1.9
3,307,354 - pnp b3164 0.14 +2.3
3,307,359 - pnp b3164 0.14 +1.8
3,307,371 - pnp b3164 0.15 -0.8
3,307,471 - pnp b3164 0.19 +3.2
3,309,216 - +0.6
3,309,239 - +1.1
3,309,437 - +0.5
3,309,823 - +0.2
3,309,823 - -1.4
3,309,838 - -0.0
3,309,987 - truB b3166 0.14 +2.2
3,309,990 - truB b3166 0.14 +1.2
3,310,065 + truB b3166 0.22 -2.0
3,310,085 - truB b3166 0.24 +1.7
3,310,126 + truB b3166 0.29 +1.0
3,310,148 + truB b3166 0.31 +0.3
3,310,148 + truB b3166 0.31 -0.1

Or see this region's nucleotide sequence