Experiment: carbon source D-Glucose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mltA and metZ are separated by 208 nucleotides metZ and metW are separated by 33 nucleotides metW and metV are separated by 33 nucleotides metV and b2817 are separated by 73 nucleotides b2817 and argA are separated by 231 nucleotides
b2813: mltA - membrane-bound lytic murein transglycosylase A (NCBI), at 2,944,103 to 2,945,200
mltA
b2814: metZ - tRNA-Met (NCBI), at 2,945,409 to 2,945,485
metZ
b2815: metW - tRNA-Met (NCBI), at 2,945,519 to 2,945,595
metW
b2816: metV - tRNA-Met (NCBI), at 2,945,629 to 2,945,705
metV
b2817: b2817 - putative amidase (VIMSS), at 2,945,779 to 2,947,032
b2817
b2818: argA - N-acetylglutamate synthase (NCBI), at 2,947,264 to 2,948,595
argA
Position (kb)
2945
2946
2947
2948 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2944.860 kb on + strand, within mltA at 2944.860 kb on + strand, within mltA at 2944.883 kb on - strand, within mltA at 2944.883 kb on - strand, within mltA at 2944.943 kb on + strand, within mltA at 2944.943 kb on + strand, within mltA at 2944.943 kb on + strand, within mltA at 2945.034 kb on + strand, within mltA at 2945.034 kb on + strand, within mltA at 2945.094 kb on - strand at 2945.105 kb on + strand at 2945.105 kb on + strand at 2945.124 kb on + strand at 2945.184 kb on + strand at 2945.272 kb on + strand at 2945.423 kb on + strand, within metZ at 2945.508 kb on - strand at 2946.029 kb on + strand, within b2817 at 2946.029 kb on + strand, within b2817 at 2946.068 kb on - strand, within b2817 at 2946.068 kb on - strand, within b2817 at 2946.086 kb on + strand, within b2817 at 2946.094 kb on + strand, within b2817 at 2946.164 kb on + strand, within b2817 at 2946.172 kb on - strand, within b2817 at 2946.213 kb on + strand, within b2817 at 2946.223 kb on + strand, within b2817 at 2946.223 kb on + strand, within b2817 at 2946.267 kb on + strand, within b2817 at 2946.309 kb on + strand, within b2817 at 2946.334 kb on - strand, within b2817 at 2946.542 kb on + strand, within b2817 at 2946.607 kb on - strand, within b2817 at 2946.610 kb on - strand, within b2817 at 2946.610 kb on - strand, within b2817 at 2946.857 kb on - strand, within b2817 at 2947.024 kb on - strand at 2947.032 kb on + strand at 2947.273 kb on - strand at 2947.422 kb on + strand, within argA at 2947.436 kb on + strand, within argA at 2947.442 kb on - strand, within argA at 2947.514 kb on - strand, within argA at 2947.514 kb on - strand, within argA at 2947.517 kb on + strand, within argA at 2947.517 kb on + strand, within argA at 2947.584 kb on - strand, within argA at 2947.584 kb on - strand, within argA at 2947.611 kb on + strand, within argA at 2947.621 kb on + strand, within argA at 2947.631 kb on - strand, within argA at 2947.748 kb on + strand, within argA at 2947.766 kb on + strand, within argA at 2947.782 kb on - strand, within argA at 2947.782 kb on - strand, within argA at 2947.783 kb on + strand, within argA
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 20mM remove 2,944,860 + mltA b2813 0.69 -1.4 2,944,860 + mltA b2813 0.69 -1.4 2,944,883 - mltA b2813 0.71 -2.7 2,944,883 - mltA b2813 0.71 -1.2 2,944,943 + mltA b2813 0.77 -0.7 2,944,943 + mltA b2813 0.77 +0.2 2,944,943 + mltA b2813 0.77 -0.0 2,945,034 + mltA b2813 0.85 -0.4 2,945,034 + mltA b2813 0.85 +0.3 2,945,094 - +0.1 2,945,105 + -0.4 2,945,105 + -0.1 2,945,124 + +1.0 2,945,184 + +0.6 2,945,272 + +0.9 2,945,423 + metZ b2814 0.18 -1.0 2,945,508 - +0.2 2,946,029 + b2817 b2817 0.20 +2.6 2,946,029 + b2817 b2817 0.20 +2.0 2,946,068 - b2817 b2817 0.23 +0.8 2,946,068 - b2817 b2817 0.23 -0.8 2,946,086 + b2817 b2817 0.24 +0.3 2,946,094 + b2817 b2817 0.25 -1.1 2,946,164 + b2817 b2817 0.31 +1.4 2,946,172 - b2817 b2817 0.31 +3.0 2,946,213 + b2817 b2817 0.35 +1.0 2,946,223 + b2817 b2817 0.35 +0.2 2,946,223 + b2817 b2817 0.35 +2.3 2,946,267 + b2817 b2817 0.39 -0.1 2,946,309 + b2817 b2817 0.42 +0.8 2,946,334 - b2817 b2817 0.44 +1.7 2,946,542 + b2817 b2817 0.61 -0.8 2,946,607 - b2817 b2817 0.66 +0.1 2,946,610 - b2817 b2817 0.66 +1.0 2,946,610 - b2817 b2817 0.66 +2.0 2,946,857 - b2817 b2817 0.86 +1.9 2,947,024 - +0.1 2,947,032 + +0.2 2,947,273 - -4.0 2,947,422 + argA b2818 0.12 -5.0 2,947,436 + argA b2818 0.13 -3.6 2,947,442 - argA b2818 0.13 -3.4 2,947,514 - argA b2818 0.19 -5.1 2,947,514 - argA b2818 0.19 -5.4 2,947,517 + argA b2818 0.19 -4.5 2,947,517 + argA b2818 0.19 -2.7 2,947,584 - argA b2818 0.24 -5.1 2,947,584 - argA b2818 0.24 -3.9 2,947,611 + argA b2818 0.26 -4.8 2,947,621 + argA b2818 0.27 -2.8 2,947,631 - argA b2818 0.28 -5.1 2,947,748 + argA b2818 0.36 -2.9 2,947,766 + argA b2818 0.38 -4.8 2,947,782 - argA b2818 0.39 -3.0 2,947,782 - argA b2818 0.39 -3.1 2,947,783 + argA b2818 0.39 -2.5
Or see this region's nucleotide sequence