Strain Fitness in Escherichia coli BW25113 around b2516

Experiment: carbon source D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntispG and yfgA are separated by 26 nucleotidesyfgA and yfgB are separated by 284 nucleotides b2515: ispG - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (NCBI), at 2,638,708 to 2,639,826 ispG b2516: yfgA - hypothetical protein (NCBI), at 2,639,853 to 2,640,866 yfgA b2517: yfgB - predicted enzyme (NCBI), at 2,641,151 to 2,642,305 yfgB Position (kb) 2639 2640 2641Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2639.871 kb on - strandat 2639.871 kb on - strandat 2639.924 kb on + strandat 2639.926 kb on - strandat 2640.019 kb on + strand, within yfgAat 2640.019 kb on + strand, within yfgAat 2640.027 kb on - strand, within yfgAat 2640.027 kb on - strand, within yfgAat 2640.027 kb on - strand, within yfgAat 2640.037 kb on - strand, within yfgAat 2640.037 kb on - strand, within yfgAat 2640.071 kb on - strand, within yfgAat 2640.129 kb on + strand, within yfgAat 2640.129 kb on + strand, within yfgAat 2640.146 kb on - strand, within yfgAat 2640.158 kb on + strand, within yfgAat 2640.262 kb on - strand, within yfgAat 2640.262 kb on - strand, within yfgAat 2640.310 kb on + strand, within yfgAat 2640.315 kb on - strand, within yfgAat 2640.336 kb on + strand, within yfgAat 2640.336 kb on + strand, within yfgAat 2640.347 kb on + strand, within yfgAat 2640.347 kb on + strand, within yfgAat 2640.347 kb on + strand, within yfgAat 2640.348 kb on + strand, within yfgAat 2640.384 kb on + strand, within yfgAat 2640.387 kb on - strand, within yfgAat 2640.442 kb on + strand, within yfgAat 2640.442 kb on + strand, within yfgAat 2640.953 kb on + strandat 2640.955 kb on + strandat 2640.963 kb on + strandat 2640.963 kb on + strandat 2640.963 kb on + strandat 2640.963 kb on + strandat 2640.963 kb on + strandat 2640.963 kb on + strandat 2640.971 kb on - strandat 2640.971 kb on - strandat 2641.026 kb on - strandat 2641.028 kb on + strandat 2641.038 kb on + strandat 2641.046 kb on - strandat 2641.046 kb on - strandat 2641.046 kb on - strandat 2641.173 kb on + strandat 2641.179 kb on + strandat 2641.181 kb on - strandat 2641.181 kb on - strandat 2641.187 kb on - strandat 2641.279 kb on + strand, within yfgBat 2641.279 kb on + strand, within yfgBat 2641.299 kb on - strand, within yfgBat 2641.344 kb on + strand, within yfgBat 2641.397 kb on - strand, within yfgBat 2641.453 kb on - strand, within yfgBat 2641.453 kb on - strand, within yfgBat 2641.671 kb on + strand, within yfgBat 2641.688 kb on + strand, within yfgBat 2641.748 kb on + strand, within yfgBat 2641.748 kb on + strand, within yfgBat 2641.784 kb on - strand, within yfgBat 2641.819 kb on + strand, within yfgBat 2641.819 kb on + strand, within yfgB

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 20mM
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2,639,871 - +1.0
2,639,871 - +0.9
2,639,924 + +1.5
2,639,926 - +0.1
2,640,019 + yfgA b2516 0.16 +2.2
2,640,019 + yfgA b2516 0.16 +1.8
2,640,027 - yfgA b2516 0.17 -0.3
2,640,027 - yfgA b2516 0.17 +0.7
2,640,027 - yfgA b2516 0.17 +0.5
2,640,037 - yfgA b2516 0.18 +0.8
2,640,037 - yfgA b2516 0.18 +2.5
2,640,071 - yfgA b2516 0.21 +1.3
2,640,129 + yfgA b2516 0.27 +0.9
2,640,129 + yfgA b2516 0.27 +1.3
2,640,146 - yfgA b2516 0.29 +2.0
2,640,158 + yfgA b2516 0.30 +2.5
2,640,262 - yfgA b2516 0.40 +0.1
2,640,262 - yfgA b2516 0.40 -0.1
2,640,310 + yfgA b2516 0.45 +1.4
2,640,315 - yfgA b2516 0.46 -1.3
2,640,336 + yfgA b2516 0.48 +1.8
2,640,336 + yfgA b2516 0.48 +1.5
2,640,347 + yfgA b2516 0.49 +1.7
2,640,347 + yfgA b2516 0.49 +0.8
2,640,347 + yfgA b2516 0.49 +0.7
2,640,348 + yfgA b2516 0.49 +2.5
2,640,384 + yfgA b2516 0.52 -2.7
2,640,387 - yfgA b2516 0.53 +0.1
2,640,442 + yfgA b2516 0.58 +2.0
2,640,442 + yfgA b2516 0.58 +0.5
2,640,953 + +2.4
2,640,955 + +1.7
2,640,963 + -1.0
2,640,963 + +0.0
2,640,963 + +0.1
2,640,963 + +1.6
2,640,963 + -1.4
2,640,963 + -0.6
2,640,971 - -0.1
2,640,971 - +0.4
2,641,026 - +0.5
2,641,028 + +2.0
2,641,038 + -0.6
2,641,046 - -0.5
2,641,046 - -0.3
2,641,046 - +1.0
2,641,173 + -0.6
2,641,179 + -0.1
2,641,181 - -0.2
2,641,181 - +0.0
2,641,187 - +1.0
2,641,279 + yfgB b2517 0.11 +0.3
2,641,279 + yfgB b2517 0.11 -0.8
2,641,299 - yfgB b2517 0.13 +0.6
2,641,344 + yfgB b2517 0.17 -3.3
2,641,397 - yfgB b2517 0.21 +0.2
2,641,453 - yfgB b2517 0.26 +0.0
2,641,453 - yfgB b2517 0.26 -0.9
2,641,671 + yfgB b2517 0.45 -0.7
2,641,688 + yfgB b2517 0.46 -1.1
2,641,748 + yfgB b2517 0.52 -2.7
2,641,748 + yfgB b2517 0.52 +0.1
2,641,784 - yfgB b2517 0.55 +0.6
2,641,819 + yfgB b2517 0.58 -0.4
2,641,819 + yfgB b2517 0.58 -1.3

Or see this region's nucleotide sequence