Experiment: carbon source D-Glucose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdxK and yfeK are separated by 104 nucleotides yfeK and yfeS are separated by 32 nucleotides yfeS and cysM are separated by 188 nucleotides
b2418: pdxK - pyridoxine kinase (NCBI), at 2,534,408 to 2,535,259
pdxK
b2419: yfeK - hypothetical protein (NCBI), at 2,535,364 to 2,535,738
yfeK
b2420: yfeS - hypothetical protein (NCBI), at 2,535,771 to 2,536,505
yfeS
b2421: cysM - cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI), at 2,536,694 to 2,537,605
cysM
Position (kb)
2535
2536
2537 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2534.980 kb on + strand, within pdxK at 2535.016 kb on + strand, within pdxK at 2535.103 kb on - strand, within pdxK at 2535.120 kb on + strand, within pdxK at 2535.137 kb on + strand, within pdxK at 2535.137 kb on + strand, within pdxK at 2535.230 kb on - strand at 2535.230 kb on - strand at 2535.230 kb on - strand at 2535.230 kb on - strand at 2535.230 kb on - strand at 2535.230 kb on - strand at 2535.230 kb on - strand at 2535.230 kb on - strand at 2535.396 kb on + strand at 2535.397 kb on - strand at 2535.447 kb on + strand, within yfeK at 2535.506 kb on - strand, within yfeK at 2535.716 kb on - strand at 2535.916 kb on + strand, within yfeS at 2536.076 kb on + strand, within yfeS at 2536.093 kb on - strand, within yfeS at 2536.093 kb on - strand, within yfeS at 2536.129 kb on + strand, within yfeS at 2536.213 kb on + strand, within yfeS at 2536.254 kb on - strand, within yfeS at 2536.282 kb on - strand, within yfeS at 2536.292 kb on - strand, within yfeS at 2536.299 kb on + strand, within yfeS at 2536.299 kb on + strand, within yfeS at 2536.307 kb on - strand, within yfeS at 2536.307 kb on - strand, within yfeS at 2536.319 kb on + strand, within yfeS at 2536.329 kb on - strand, within yfeS at 2536.340 kb on + strand, within yfeS at 2536.343 kb on - strand, within yfeS at 2536.370 kb on + strand, within yfeS at 2536.370 kb on + strand, within yfeS at 2536.448 kb on - strand at 2536.448 kb on - strand at 2536.531 kb on + strand at 2536.700 kb on - strand at 2536.702 kb on - strand at 2536.906 kb on + strand, within cysM at 2537.014 kb on - strand, within cysM at 2537.014 kb on - strand, within cysM at 2537.041 kb on + strand, within cysM at 2537.041 kb on + strand, within cysM at 2537.148 kb on + strand, within cysM at 2537.148 kb on + strand, within cysM
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 20mM remove 2,534,980 + pdxK b2418 0.67 +0.2 2,535,016 + pdxK b2418 0.71 -1.8 2,535,103 - pdxK b2418 0.82 +0.3 2,535,120 + pdxK b2418 0.84 -3.8 2,535,137 + pdxK b2418 0.86 +0.5 2,535,137 + pdxK b2418 0.86 -1.4 2,535,230 - +0.0 2,535,230 - +0.2 2,535,230 - +0.1 2,535,230 - -3.0 2,535,230 - -1.5 2,535,230 - -1.7 2,535,230 - -1.4 2,535,230 - +0.5 2,535,396 + +1.5 2,535,397 - +0.2 2,535,447 + yfeK b2419 0.22 +1.4 2,535,506 - yfeK b2419 0.38 +0.1 2,535,716 - +0.0 2,535,916 + yfeS b2420 0.20 +0.3 2,536,076 + yfeS b2420 0.41 -1.0 2,536,093 - yfeS b2420 0.44 +1.6 2,536,093 - yfeS b2420 0.44 +1.6 2,536,129 + yfeS b2420 0.49 +1.0 2,536,213 + yfeS b2420 0.60 -0.1 2,536,254 - yfeS b2420 0.66 -1.6 2,536,282 - yfeS b2420 0.70 +2.5 2,536,292 - yfeS b2420 0.71 +1.6 2,536,299 + yfeS b2420 0.72 +0.5 2,536,299 + yfeS b2420 0.72 +0.7 2,536,307 - yfeS b2420 0.73 -2.1 2,536,307 - yfeS b2420 0.73 +1.0 2,536,319 + yfeS b2420 0.75 -0.4 2,536,329 - yfeS b2420 0.76 -0.3 2,536,340 + yfeS b2420 0.77 +0.6 2,536,343 - yfeS b2420 0.78 +0.6 2,536,370 + yfeS b2420 0.81 +1.2 2,536,370 + yfeS b2420 0.81 +2.2 2,536,448 - -2.1 2,536,448 - +0.0 2,536,531 + -1.4 2,536,700 - +0.1 2,536,702 - -0.2 2,536,906 + cysM b2421 0.23 +0.3 2,537,014 - cysM b2421 0.35 +0.5 2,537,014 - cysM b2421 0.35 +0.2 2,537,041 + cysM b2421 0.38 +1.4 2,537,041 + cysM b2421 0.38 -0.4 2,537,148 + cysM b2421 0.50 -0.1 2,537,148 + cysM b2421 0.50 -0.3
Or see this region's nucleotide sequence