Strain Fitness in Escherichia coli BW25113 around b2420

Experiment: carbon source D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpdxK and yfeK are separated by 104 nucleotidesyfeK and yfeS are separated by 32 nucleotidesyfeS and cysM are separated by 188 nucleotides b2418: pdxK - pyridoxine kinase (NCBI), at 2,534,408 to 2,535,259 pdxK b2419: yfeK - hypothetical protein (NCBI), at 2,535,364 to 2,535,738 yfeK b2420: yfeS - hypothetical protein (NCBI), at 2,535,771 to 2,536,505 yfeS b2421: cysM - cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI), at 2,536,694 to 2,537,605 cysM Position (kb) 2535 2536 2537Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2534.980 kb on + strand, within pdxKat 2535.016 kb on + strand, within pdxKat 2535.103 kb on - strand, within pdxKat 2535.120 kb on + strand, within pdxKat 2535.137 kb on + strand, within pdxKat 2535.137 kb on + strand, within pdxKat 2535.230 kb on - strandat 2535.230 kb on - strandat 2535.230 kb on - strandat 2535.230 kb on - strandat 2535.230 kb on - strandat 2535.230 kb on - strandat 2535.230 kb on - strandat 2535.230 kb on - strandat 2535.396 kb on + strandat 2535.397 kb on - strandat 2535.447 kb on + strand, within yfeKat 2535.506 kb on - strand, within yfeKat 2535.716 kb on - strandat 2535.916 kb on + strand, within yfeSat 2536.076 kb on + strand, within yfeSat 2536.093 kb on - strand, within yfeSat 2536.093 kb on - strand, within yfeSat 2536.129 kb on + strand, within yfeSat 2536.213 kb on + strand, within yfeSat 2536.254 kb on - strand, within yfeSat 2536.282 kb on - strand, within yfeSat 2536.292 kb on - strand, within yfeSat 2536.299 kb on + strand, within yfeSat 2536.299 kb on + strand, within yfeSat 2536.307 kb on - strand, within yfeSat 2536.307 kb on - strand, within yfeSat 2536.319 kb on + strand, within yfeSat 2536.329 kb on - strand, within yfeSat 2536.340 kb on + strand, within yfeSat 2536.343 kb on - strand, within yfeSat 2536.370 kb on + strand, within yfeSat 2536.370 kb on + strand, within yfeSat 2536.448 kb on - strandat 2536.448 kb on - strandat 2536.531 kb on + strandat 2536.700 kb on - strandat 2536.702 kb on - strandat 2536.906 kb on + strand, within cysMat 2537.014 kb on - strand, within cysMat 2537.014 kb on - strand, within cysMat 2537.041 kb on + strand, within cysMat 2537.041 kb on + strand, within cysMat 2537.148 kb on + strand, within cysMat 2537.148 kb on + strand, within cysM

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 20mM
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2,534,980 + pdxK b2418 0.67 +0.2
2,535,016 + pdxK b2418 0.71 -1.8
2,535,103 - pdxK b2418 0.82 +0.3
2,535,120 + pdxK b2418 0.84 -3.8
2,535,137 + pdxK b2418 0.86 +0.5
2,535,137 + pdxK b2418 0.86 -1.4
2,535,230 - +0.0
2,535,230 - +0.2
2,535,230 - +0.1
2,535,230 - -3.0
2,535,230 - -1.5
2,535,230 - -1.7
2,535,230 - -1.4
2,535,230 - +0.5
2,535,396 + +1.5
2,535,397 - +0.2
2,535,447 + yfeK b2419 0.22 +1.4
2,535,506 - yfeK b2419 0.38 +0.1
2,535,716 - +0.0
2,535,916 + yfeS b2420 0.20 +0.3
2,536,076 + yfeS b2420 0.41 -1.0
2,536,093 - yfeS b2420 0.44 +1.6
2,536,093 - yfeS b2420 0.44 +1.6
2,536,129 + yfeS b2420 0.49 +1.0
2,536,213 + yfeS b2420 0.60 -0.1
2,536,254 - yfeS b2420 0.66 -1.6
2,536,282 - yfeS b2420 0.70 +2.5
2,536,292 - yfeS b2420 0.71 +1.6
2,536,299 + yfeS b2420 0.72 +0.5
2,536,299 + yfeS b2420 0.72 +0.7
2,536,307 - yfeS b2420 0.73 -2.1
2,536,307 - yfeS b2420 0.73 +1.0
2,536,319 + yfeS b2420 0.75 -0.4
2,536,329 - yfeS b2420 0.76 -0.3
2,536,340 + yfeS b2420 0.77 +0.6
2,536,343 - yfeS b2420 0.78 +0.6
2,536,370 + yfeS b2420 0.81 +1.2
2,536,370 + yfeS b2420 0.81 +2.2
2,536,448 - -2.1
2,536,448 - +0.0
2,536,531 + -1.4
2,536,700 - +0.1
2,536,702 - -0.2
2,536,906 + cysM b2421 0.23 +0.3
2,537,014 - cysM b2421 0.35 +0.5
2,537,014 - cysM b2421 0.35 +0.2
2,537,041 + cysM b2421 0.38 +1.4
2,537,041 + cysM b2421 0.38 -0.4
2,537,148 + cysM b2421 0.50 -0.1
2,537,148 + cysM b2421 0.50 -0.3

Or see this region's nucleotide sequence