Experiment: carbon source D-Glucose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt racR and ydaS are separated by 123 nucleotides ydaS and ydaT are separated by 22 nucleotides ydaT and ydaU are separated by 12 nucleotides ydaU and ydaV are separated by 6 nucleotides ydaV and ydaW overlap by 29 nucleotides
b1356: racR - Rac prophage; predicted DNA-binding transcriptional regulator (NCBI), at 1,417,789 to 1,418,265
racR
b1357: ydaS - Rac prophage; predicted DNA-binding transcriptional regulator (NCBI), at 1,418,389 to 1,418,685
ydaS
b1358: ydaT - Rac prophage; predicted protein (NCBI), at 1,418,708 to 1,419,130
ydaT
b1359: ydaU - Rac prophage; conserved protein (NCBI), at 1,419,143 to 1,420,000
ydaU
b1360: ydaV - Rac prophage; predicted DNA replication protein (NCBI), at 1,420,007 to 1,420,753
ydaV
b1361: ydaW - orf, hypothetical protein (VIMSS), at 1,420,725 to 1,421,224
ydaW
Position (kb)
1419
1420
1421 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1418.573 kb on - strand, within ydaS at 1418.619 kb on - strand, within ydaS at 1418.746 kb on - strand at 1418.848 kb on - strand, within ydaT at 1418.848 kb on - strand, within ydaT at 1419.186 kb on + strand at 1419.186 kb on + strand at 1419.235 kb on - strand, within ydaU at 1419.360 kb on - strand, within ydaU at 1419.393 kb on - strand, within ydaU at 1419.393 kb on - strand, within ydaU at 1419.797 kb on + strand, within ydaU at 1419.910 kb on + strand, within ydaU at 1419.933 kb on - strand at 1419.988 kb on - strand at 1420.068 kb on - strand at 1420.436 kb on - strand, within ydaV at 1420.589 kb on + strand, within ydaV at 1420.831 kb on - strand, within ydaW
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 20mM remove 1,418,573 - ydaS b1357 0.62 -0.7 1,418,619 - ydaS b1357 0.77 -0.4 1,418,746 - +0.8 1,418,848 - ydaT b1358 0.33 +1.7 1,418,848 - ydaT b1358 0.33 -1.6 1,419,186 + +1.3 1,419,186 + +0.7 1,419,235 - ydaU b1359 0.11 -0.2 1,419,360 - ydaU b1359 0.25 +0.3 1,419,393 - ydaU b1359 0.29 +3.1 1,419,393 - ydaU b1359 0.29 -1.9 1,419,797 + ydaU b1359 0.76 +0.5 1,419,910 + ydaU b1359 0.89 +1.0 1,419,933 - +0.3 1,419,988 - +1.8 1,420,068 - -1.2 1,420,436 - ydaV b1360 0.57 -1.6 1,420,589 + ydaV b1360 0.78 -0.0 1,420,831 - ydaW b1361 0.21 -1.6
Or see this region's nucleotide sequence