Experiment: carbon source D-Glucose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fabF and pabC are separated by 119 nucleotides pabC and yceG are separated by 2 nucleotides yceG and tmk overlap by 11 nucleotides tmk and holB overlap by 4 nucleotides
b1095: fabF - 3-oxoacyl-(acyl carrier protein) synthase (NCBI), at 1,151,162 to 1,152,403
fabF
b1096: pabC - 4-amino-4-deoxychorismate lyase (NCBI), at 1,152,523 to 1,153,332
pabC
b1097: yceG - predicted aminodeoxychorismate lyase (NCBI), at 1,153,335 to 1,154,357
yceG
b1098: tmk - thymidylate kinase (NCBI), at 1,154,347 to 1,154,988
tmk
b1099: holB - DNA polymerase III subunit delta' (NCBI), at 1,154,985 to 1,155,989
holB
Position (kb)
1153
1154
1155 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1152.335 kb on + strand at 1152.361 kb on - strand at 1152.361 kb on - strand at 1152.420 kb on + strand at 1152.420 kb on + strand at 1152.474 kb on - strand at 1152.482 kb on - strand at 1152.520 kb on + strand at 1152.520 kb on + strand at 1152.558 kb on + strand at 1152.676 kb on - strand, within pabC at 1152.788 kb on + strand, within pabC at 1152.788 kb on + strand, within pabC at 1152.876 kb on + strand, within pabC at 1152.902 kb on - strand, within pabC at 1152.909 kb on + strand, within pabC at 1152.909 kb on + strand, within pabC at 1153.012 kb on + strand, within pabC at 1153.171 kb on + strand, within pabC at 1153.177 kb on + strand, within pabC at 1153.208 kb on - strand, within pabC at 1153.223 kb on + strand, within pabC at 1153.301 kb on - strand at 1153.412 kb on + strand at 1153.412 kb on + strand at 1153.585 kb on - strand, within yceG at 1153.597 kb on + strand, within yceG at 1153.603 kb on - strand, within yceG at 1153.609 kb on + strand, within yceG at 1153.656 kb on + strand, within yceG at 1153.697 kb on - strand, within yceG at 1153.802 kb on + strand, within yceG at 1153.907 kb on + strand, within yceG at 1153.907 kb on + strand, within yceG at 1153.934 kb on + strand, within yceG at 1154.104 kb on - strand, within yceG at 1154.150 kb on + strand, within yceG at 1154.150 kb on + strand, within yceG at 1154.152 kb on - strand, within yceG at 1154.152 kb on - strand, within yceG at 1154.158 kb on - strand, within yceG at 1154.158 kb on - strand, within yceG at 1154.160 kb on - strand, within yceG at 1154.280 kb on - strand at 1154.323 kb on + strand at 1154.323 kb on + strand at 1155.056 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 20mM remove 1,152,335 + +0.3 1,152,361 - +1.4 1,152,361 - +0.3 1,152,420 + -1.0 1,152,420 + -0.8 1,152,474 - -0.2 1,152,482 - +0.0 1,152,520 + -1.5 1,152,520 + +1.3 1,152,558 + -0.5 1,152,676 - pabC b1096 0.19 -0.3 1,152,788 + pabC b1096 0.33 -0.1 1,152,788 + pabC b1096 0.33 -0.5 1,152,876 + pabC b1096 0.44 +3.5 1,152,902 - pabC b1096 0.47 -1.1 1,152,909 + pabC b1096 0.48 -2.3 1,152,909 + pabC b1096 0.48 +1.5 1,153,012 + pabC b1096 0.60 -0.9 1,153,171 + pabC b1096 0.80 -0.0 1,153,177 + pabC b1096 0.81 -1.4 1,153,208 - pabC b1096 0.85 -1.5 1,153,223 + pabC b1096 0.86 -1.0 1,153,301 - -0.2 1,153,412 + +0.5 1,153,412 + +0.7 1,153,585 - yceG b1097 0.24 -2.2 1,153,597 + yceG b1097 0.26 -0.1 1,153,603 - yceG b1097 0.26 +1.2 1,153,609 + yceG b1097 0.27 -0.6 1,153,656 + yceG b1097 0.31 -0.5 1,153,697 - yceG b1097 0.35 +0.7 1,153,802 + yceG b1097 0.46 +0.1 1,153,907 + yceG b1097 0.56 +0.1 1,153,907 + yceG b1097 0.56 +1.0 1,153,934 + yceG b1097 0.59 +1.5 1,154,104 - yceG b1097 0.75 +1.2 1,154,150 + yceG b1097 0.80 +0.0 1,154,150 + yceG b1097 0.80 +0.4 1,154,152 - yceG b1097 0.80 +1.4 1,154,152 - yceG b1097 0.80 +1.2 1,154,158 - yceG b1097 0.80 -2.0 1,154,158 - yceG b1097 0.80 +3.8 1,154,160 - yceG b1097 0.81 +0.7 1,154,280 - +0.0 1,154,323 + +0.2 1,154,323 + -0.7 1,155,056 + +0.5
Or see this region's nucleotide sequence