Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_16670 and OKGIIK_16675 overlap by 4 nucleotides OKGIIK_16675 and natA overlap by 4 nucleotides natA and natB overlap by 4 nucleotides natB and OKGIIK_16690 are separated by 182 nucleotides OKGIIK_16690 and folE are separated by 26 nucleotides
OKGIIK_16670: OKGIIK_16670 - Transporter, at 3,659,229 to 3,660,806
_16670
OKGIIK_16675: OKGIIK_16675 - hypothetical protein, at 3,660,803 to 3,660,976
_16675
OKGIIK_16680: natA - ABC transporter, at 3,660,973 to 3,661,707
natA
OKGIIK_16685: natB - ABC transporter permease, at 3,661,704 to 3,662,906
natB
OKGIIK_16690: OKGIIK_16690 - EF-hand domain-containing protein, at 3,663,089 to 3,663,391
_16690
OKGIIK_16695: folE - GTP cyclohydrolase I FolE, at 3,663,418 to 3,664,020
folE
Position (kb)
3661
3662
3663 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3660.815 kb on + strand at 3660.964 kb on + strand at 3660.964 kb on + strand at 3660.964 kb on + strand at 3660.964 kb on + strand at 3660.965 kb on - strand at 3660.965 kb on - strand at 3660.965 kb on - strand at 3661.114 kb on + strand, within natA at 3661.114 kb on + strand, within natA at 3661.114 kb on + strand, within natA at 3661.114 kb on + strand, within natA at 3661.114 kb on + strand, within natA at 3661.114 kb on + strand, within natA at 3661.114 kb on + strand, within natA at 3661.114 kb on + strand, within natA at 3661.115 kb on - strand, within natA at 3661.115 kb on - strand, within natA at 3661.115 kb on - strand, within natA at 3661.228 kb on + strand, within natA at 3661.228 kb on + strand, within natA at 3661.228 kb on + strand, within natA at 3661.228 kb on + strand, within natA at 3661.228 kb on + strand, within natA at 3661.229 kb on - strand, within natA at 3661.229 kb on - strand, within natA at 3661.229 kb on - strand, within natA at 3661.229 kb on - strand, within natA at 3661.261 kb on + strand, within natA at 3661.261 kb on + strand, within natA at 3661.261 kb on + strand, within natA at 3661.261 kb on + strand, within natA at 3661.262 kb on - strand, within natA at 3661.262 kb on - strand, within natA at 3661.262 kb on - strand, within natA at 3662.094 kb on + strand, within natB at 3662.094 kb on + strand, within natB at 3662.094 kb on + strand, within natB at 3662.094 kb on + strand, within natB at 3662.094 kb on + strand, within natB at 3662.094 kb on + strand, within natB at 3662.157 kb on + strand, within natB at 3662.157 kb on + strand, within natB at 3662.157 kb on + strand, within natB at 3662.158 kb on - strand, within natB at 3662.158 kb on - strand, within natB at 3662.158 kb on - strand, within natB at 3662.292 kb on + strand, within natB at 3662.292 kb on + strand, within natB at 3662.292 kb on + strand, within natB at 3662.292 kb on + strand, within natB at 3662.292 kb on + strand, within natB at 3662.293 kb on - strand, within natB at 3662.293 kb on - strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.329 kb on - strand, within natB at 3662.329 kb on - strand, within natB at 3662.329 kb on - strand, within natB at 3662.329 kb on - strand, within natB at 3662.329 kb on - strand, within natB at 3662.329 kb on - strand, within natB at 3662.329 kb on - strand, within natB at 3662.329 kb on - strand, within natB at 3662.635 kb on - strand, within natB at 3662.652 kb on + strand, within natB at 3662.652 kb on + strand, within natB at 3662.652 kb on + strand, within natB at 3662.652 kb on + strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.733 kb on + strand, within natB at 3662.733 kb on + strand, within natB at 3662.733 kb on + strand, within natB at 3662.733 kb on + strand, within natB at 3662.733 kb on + strand, within natB at 3662.734 kb on - strand, within natB at 3662.734 kb on - strand, within natB at 3662.734 kb on - strand, within natB at 3662.734 kb on - strand, within natB at 3662.734 kb on - strand, within natB at 3662.734 kb on - strand, within natB at 3662.734 kb on - strand, within natB at 3662.734 kb on - strand, within natB at 3662.874 kb on + strand at 3662.874 kb on + strand at 3662.874 kb on + strand at 3662.874 kb on + strand at 3662.916 kb on + strand at 3662.917 kb on - strand at 3662.998 kb on + strand at 3662.998 kb on + strand at 3662.998 kb on + strand at 3662.998 kb on + strand at 3662.998 kb on + strand at 3662.998 kb on + strand at 3662.998 kb on + strand at 3662.999 kb on - strand at 3662.999 kb on - strand at 3662.999 kb on - strand at 3662.999 kb on - strand at 3662.999 kb on - strand at 3662.999 kb on - strand at 3662.999 kb on - strand at 3663.070 kb on + strand at 3663.070 kb on + strand at 3663.070 kb on + strand at 3663.070 kb on + strand at 3663.070 kb on + strand at 3663.070 kb on + strand at 3663.070 kb on + strand at 3663.070 kb on + strand at 3663.071 kb on - strand at 3663.071 kb on - strand at 3663.071 kb on - strand at 3663.071 kb on - strand at 3663.071 kb on - strand at 3663.087 kb on + strand at 3663.126 kb on + strand, within OKGIIK_16690 at 3663.127 kb on - strand, within OKGIIK_16690 at 3663.180 kb on + strand, within OKGIIK_16690 at 3663.180 kb on + strand, within OKGIIK_16690 at 3663.180 kb on + strand, within OKGIIK_16690 at 3663.181 kb on - strand, within OKGIIK_16690 at 3663.181 kb on - strand, within OKGIIK_16690 at 3663.282 kb on + strand, within OKGIIK_16690 at 3663.282 kb on + strand, within OKGIIK_16690 at 3663.282 kb on + strand, within OKGIIK_16690 at 3663.282 kb on + strand, within OKGIIK_16690 at 3663.282 kb on + strand, within OKGIIK_16690 at 3663.282 kb on + strand, within OKGIIK_16690 at 3663.282 kb on + strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.291 kb on + strand, within OKGIIK_16690 at 3663.291 kb on + strand, within OKGIIK_16690 at 3663.291 kb on + strand, within OKGIIK_16690 at 3663.291 kb on + strand, within OKGIIK_16690 at 3663.292 kb on - strand, within OKGIIK_16690 at 3663.292 kb on - strand, within OKGIIK_16690 at 3663.292 kb on - strand, within OKGIIK_16690 at 3663.292 kb on - strand, within OKGIIK_16690 at 3663.292 kb on - strand, within OKGIIK_16690 at 3663.342 kb on + strand, within OKGIIK_16690 at 3663.342 kb on + strand, within OKGIIK_16690 at 3663.342 kb on + strand, within OKGIIK_16690
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 3,660,815 + -0.1 3,660,964 + +0.5 3,660,964 + +0.7 3,660,964 + -0.5 3,660,964 + +0.4 3,660,965 - -1.4 3,660,965 - -0.7 3,660,965 - -1.0 3,661,114 + natA OKGIIK_16680 0.19 -0.3 3,661,114 + natA OKGIIK_16680 0.19 -0.5 3,661,114 + natA OKGIIK_16680 0.19 -2.1 3,661,114 + natA OKGIIK_16680 0.19 -0.9 3,661,114 + natA OKGIIK_16680 0.19 -2.4 3,661,114 + natA OKGIIK_16680 0.19 -2.3 3,661,114 + natA OKGIIK_16680 0.19 -3.1 3,661,114 + natA OKGIIK_16680 0.19 -1.0 3,661,115 - natA OKGIIK_16680 0.19 -1.5 3,661,115 - natA OKGIIK_16680 0.19 -1.1 3,661,115 - natA OKGIIK_16680 0.19 +1.0 3,661,228 + natA OKGIIK_16680 0.35 +1.0 3,661,228 + natA OKGIIK_16680 0.35 +2.0 3,661,228 + natA OKGIIK_16680 0.35 +0.0 3,661,228 + natA OKGIIK_16680 0.35 -1.3 3,661,228 + natA OKGIIK_16680 0.35 -1.5 3,661,229 - natA OKGIIK_16680 0.35 -1.0 3,661,229 - natA OKGIIK_16680 0.35 -1.0 3,661,229 - natA OKGIIK_16680 0.35 +0.5 3,661,229 - natA OKGIIK_16680 0.35 -2.0 3,661,261 + natA OKGIIK_16680 0.39 -1.5 3,661,261 + natA OKGIIK_16680 0.39 -1.0 3,661,261 + natA OKGIIK_16680 0.39 -0.8 3,661,261 + natA OKGIIK_16680 0.39 -0.5 3,661,262 - natA OKGIIK_16680 0.39 +0.0 3,661,262 - natA OKGIIK_16680 0.39 -1.6 3,661,262 - natA OKGIIK_16680 0.39 -1.3 3,662,094 + natB OKGIIK_16685 0.32 -0.3 3,662,094 + natB OKGIIK_16685 0.32 +0.3 3,662,094 + natB OKGIIK_16685 0.32 -1.0 3,662,094 + natB OKGIIK_16685 0.32 +0.0 3,662,094 + natB OKGIIK_16685 0.32 -0.5 3,662,094 + natB OKGIIK_16685 0.32 -0.5 3,662,157 + natB OKGIIK_16685 0.38 -1.8 3,662,157 + natB OKGIIK_16685 0.38 -2.3 3,662,157 + natB OKGIIK_16685 0.38 -1.8 3,662,158 - natB OKGIIK_16685 0.38 -0.5 3,662,158 - natB OKGIIK_16685 0.38 -1.0 3,662,158 - natB OKGIIK_16685 0.38 -0.5 3,662,292 + natB OKGIIK_16685 0.49 -0.5 3,662,292 + natB OKGIIK_16685 0.49 -0.2 3,662,292 + natB OKGIIK_16685 0.49 +1.0 3,662,292 + natB OKGIIK_16685 0.49 -1.8 3,662,292 + natB OKGIIK_16685 0.49 -1.5 3,662,293 - natB OKGIIK_16685 0.49 +0.0 3,662,293 - natB OKGIIK_16685 0.49 +2.0 3,662,328 + natB OKGIIK_16685 0.52 +1.4 3,662,328 + natB OKGIIK_16685 0.52 -1.5 3,662,328 + natB OKGIIK_16685 0.52 -0.5 3,662,328 + natB OKGIIK_16685 0.52 -2.0 3,662,328 + natB OKGIIK_16685 0.52 -0.5 3,662,328 + natB OKGIIK_16685 0.52 -1.9 3,662,328 + natB OKGIIK_16685 0.52 -1.3 3,662,328 + natB OKGIIK_16685 0.52 +0.0 3,662,328 + natB OKGIIK_16685 0.52 -1.8 3,662,328 + natB OKGIIK_16685 0.52 -2.3 3,662,328 + natB OKGIIK_16685 0.52 -1.3 3,662,328 + natB OKGIIK_16685 0.52 +0.0 3,662,329 - natB OKGIIK_16685 0.52 -1.8 3,662,329 - natB OKGIIK_16685 0.52 -1.0 3,662,329 - natB OKGIIK_16685 0.52 -1.5 3,662,329 - natB OKGIIK_16685 0.52 -1.2 3,662,329 - natB OKGIIK_16685 0.52 -1.5 3,662,329 - natB OKGIIK_16685 0.52 +0.0 3,662,329 - natB OKGIIK_16685 0.52 +0.6 3,662,329 - natB OKGIIK_16685 0.52 -1.8 3,662,635 - natB OKGIIK_16685 0.77 -2.5 3,662,652 + natB OKGIIK_16685 0.79 -1.5 3,662,652 + natB OKGIIK_16685 0.79 -1.2 3,662,652 + natB OKGIIK_16685 0.79 -1.1 3,662,652 + natB OKGIIK_16685 0.79 -2.8 3,662,653 - natB OKGIIK_16685 0.79 -1.5 3,662,653 - natB OKGIIK_16685 0.79 -1.5 3,662,653 - natB OKGIIK_16685 0.79 -2.0 3,662,653 - natB OKGIIK_16685 0.79 -1.5 3,662,653 - natB OKGIIK_16685 0.79 -2.1 3,662,653 - natB OKGIIK_16685 0.79 -1.0 3,662,653 - natB OKGIIK_16685 0.79 -3.4 3,662,653 - natB OKGIIK_16685 0.79 -2.7 3,662,653 - natB OKGIIK_16685 0.79 -2.1 3,662,733 + natB OKGIIK_16685 0.86 -1.0 3,662,733 + natB OKGIIK_16685 0.86 -1.0 3,662,733 + natB OKGIIK_16685 0.86 +0.6 3,662,733 + natB OKGIIK_16685 0.86 -1.0 3,662,733 + natB OKGIIK_16685 0.86 -0.1 3,662,734 - natB OKGIIK_16685 0.86 +0.3 3,662,734 - natB OKGIIK_16685 0.86 -2.9 3,662,734 - natB OKGIIK_16685 0.86 -1.3 3,662,734 - natB OKGIIK_16685 0.86 -1.0 3,662,734 - natB OKGIIK_16685 0.86 +0.0 3,662,734 - natB OKGIIK_16685 0.86 -1.8 3,662,734 - natB OKGIIK_16685 0.86 -1.0 3,662,734 - natB OKGIIK_16685 0.86 +1.0 3,662,874 + -1.3 3,662,874 + -2.9 3,662,874 + +0.4 3,662,874 + -1.0 3,662,916 + -0.5 3,662,917 - -1.0 3,662,998 + +0.0 3,662,998 + +1.2 3,662,998 + +0.6 3,662,998 + -0.3 3,662,998 + +0.7 3,662,998 + +2.6 3,662,998 + -2.3 3,662,999 - -1.3 3,662,999 - -0.2 3,662,999 - +0.7 3,662,999 - -0.8 3,662,999 - -0.5 3,662,999 - +0.4 3,662,999 - +1.4 3,663,070 + +0.5 3,663,070 + -1.8 3,663,070 + -2.1 3,663,070 + +1.3 3,663,070 + +0.4 3,663,070 + +0.0 3,663,070 + -0.4 3,663,070 + -1.5 3,663,071 - -0.4 3,663,071 - +1.5 3,663,071 - -0.3 3,663,071 - -3.0 3,663,071 - +0.0 3,663,087 + +0.5 3,663,126 + OKGIIK_16690 0.12 +1.0 3,663,127 - OKGIIK_16690 0.13 +0.6 3,663,180 + OKGIIK_16690 0.30 +0.2 3,663,180 + OKGIIK_16690 0.30 -0.5 3,663,180 + OKGIIK_16690 0.30 +1.0 3,663,181 - OKGIIK_16690 0.30 +0.0 3,663,181 - OKGIIK_16690 0.30 -1.5 3,663,282 + OKGIIK_16690 0.64 +0.0 3,663,282 + OKGIIK_16690 0.64 -1.0 3,663,282 + OKGIIK_16690 0.64 -1.0 3,663,282 + OKGIIK_16690 0.64 +0.0 3,663,282 + OKGIIK_16690 0.64 +0.5 3,663,282 + OKGIIK_16690 0.64 -1.0 3,663,282 + OKGIIK_16690 0.64 +0.5 3,663,283 - OKGIIK_16690 0.64 +1.0 3,663,283 - OKGIIK_16690 0.64 -0.5 3,663,283 - OKGIIK_16690 0.64 +2.4 3,663,283 - OKGIIK_16690 0.64 -0.1 3,663,283 - OKGIIK_16690 0.64 -2.4 3,663,283 - OKGIIK_16690 0.64 +1.0 3,663,283 - OKGIIK_16690 0.64 +0.2 3,663,291 + OKGIIK_16690 0.67 -0.3 3,663,291 + OKGIIK_16690 0.67 -0.5 3,663,291 + OKGIIK_16690 0.67 -0.4 3,663,291 + OKGIIK_16690 0.67 +0.3 3,663,292 - OKGIIK_16690 0.67 +0.0 3,663,292 - OKGIIK_16690 0.67 -0.5 3,663,292 - OKGIIK_16690 0.67 +0.6 3,663,292 - OKGIIK_16690 0.67 -0.2 3,663,292 - OKGIIK_16690 0.67 -1.5 3,663,342 + OKGIIK_16690 0.83 -0.1 3,663,342 + OKGIIK_16690 0.83 -0.5 3,663,342 + OKGIIK_16690 0.83 +0.0
Or see this region's nucleotide sequence