Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt 16S_rrna and Ile_trna are separated by 173 nucleotides Ile_trna and Ala_trna are separated by 66 nucleotides Ala_trna and 23S_rrna are separated by 190 nucleotides 23S_rrna and 5S_rrna are separated by 245 nucleotides 5S_rrna and OKGIIK_15745 are separated by 217 nucleotides OKGIIK_15745 and OKGIIK_15750 are separated by 59 nucleotides
OKGIIK_15720: 16S_rrna - 16S ribosomal RNA, at 3,434,055 to 3,435,599
_rrna
OKGIIK_15725: Ile_trna - tRNA-Ile, at 3,435,773 to 3,435,849
_trna
OKGIIK_15730: Ala_trna - tRNA-Ala, at 3,435,916 to 3,435,991
_trna
OKGIIK_15735: 23S_rrna - 23S ribosomal RNA, at 3,436,182 to 3,439,061
_rrna
OKGIIK_15740: 5S_rrna - 5S ribosomal RNA, at 3,439,307 to 3,439,420
_rrna
OKGIIK_15745: OKGIIK_15745 - hypothetical protein, at 3,439,638 to 3,439,832
_15745
OKGIIK_15750: OKGIIK_15750 - Transposase, at 3,439,892 to 3,440,185
_15750
Position (kb)
3436
3437
3438
3439
3440 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3435.851 kb on + strand at 3436.378 kb on + strand at 3436.378 kb on + strand at 3437.256 kb on + strand, within 23S_rrna at 3437.259 kb on + strand, within 23S_rrna at 3437.259 kb on + strand, within 23S_rrna at 3437.260 kb on - strand, within 23S_rrna at 3437.975 kb on - strand, within 23S_rrna at 3437.975 kb on - strand, within 23S_rrna at 3438.721 kb on + strand, within 23S_rrna at 3439.528 kb on + strand at 3439.528 kb on + strand at 3439.528 kb on + strand at 3439.528 kb on + strand at 3439.528 kb on + strand at 3439.528 kb on + strand at 3439.583 kb on + strand at 3439.583 kb on + strand at 3439.583 kb on + strand at 3439.583 kb on + strand at 3439.583 kb on + strand at 3439.583 kb on + strand at 3439.583 kb on + strand at 3439.583 kb on + strand at 3439.583 kb on + strand at 3439.583 kb on + strand at 3439.584 kb on - strand at 3439.584 kb on - strand at 3439.584 kb on - strand at 3439.584 kb on - strand at 3439.584 kb on - strand at 3439.584 kb on - strand at 3439.584 kb on - strand at 3439.609 kb on + strand at 3439.610 kb on - strand at 3439.623 kb on - strand at 3439.643 kb on + strand at 3439.643 kb on + strand at 3439.643 kb on + strand at 3439.644 kb on - strand at 3439.710 kb on + strand, within OKGIIK_15745 at 3439.710 kb on + strand, within OKGIIK_15745 at 3439.710 kb on + strand, within OKGIIK_15745 at 3439.710 kb on + strand, within OKGIIK_15745 at 3439.710 kb on + strand, within OKGIIK_15745 at 3439.710 kb on + strand, within OKGIIK_15745 at 3439.710 kb on + strand, within OKGIIK_15745 at 3439.710 kb on + strand, within OKGIIK_15745 at 3439.710 kb on + strand, within OKGIIK_15745 at 3439.710 kb on + strand, within OKGIIK_15745 at 3439.710 kb on + strand, within OKGIIK_15745 at 3439.711 kb on - strand, within OKGIIK_15745 at 3439.711 kb on - strand, within OKGIIK_15745 at 3439.711 kb on - strand, within OKGIIK_15745 at 3439.711 kb on - strand, within OKGIIK_15745 at 3439.711 kb on - strand, within OKGIIK_15745 at 3439.711 kb on - strand, within OKGIIK_15745 at 3439.711 kb on - strand, within OKGIIK_15745 at 3439.711 kb on - strand, within OKGIIK_15745 at 3439.711 kb on - strand, within OKGIIK_15745 at 3439.750 kb on - strand, within OKGIIK_15745 at 3439.852 kb on - strand at 3439.852 kb on - strand at 3439.852 kb on - strand at 3439.852 kb on - strand at 3439.852 kb on - strand at 3439.852 kb on - strand at 3439.852 kb on - strand at 3439.852 kb on - strand at 3439.852 kb on - strand at 3439.852 kb on - strand at 3439.852 kb on - strand at 3439.852 kb on - strand at 3439.852 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 3,435,851 + +1.2 3,436,378 + +1.0 3,436,378 + -0.6 3,437,256 + 23S_rrna OKGIIK_15735 0.37 -0.6 3,437,259 + 23S_rrna OKGIIK_15735 0.37 -1.0 3,437,259 + 23S_rrna OKGIIK_15735 0.37 -1.0 3,437,260 - 23S_rrna OKGIIK_15735 0.37 +1.0 3,437,975 - 23S_rrna OKGIIK_15735 0.62 -2.9 3,437,975 - 23S_rrna OKGIIK_15735 0.62 -1.0 3,438,721 + 23S_rrna OKGIIK_15735 0.88 +1.0 3,439,528 + +1.4 3,439,528 + -1.3 3,439,528 + -1.3 3,439,528 + -0.7 3,439,528 + -0.6 3,439,528 + -0.3 3,439,583 + +0.7 3,439,583 + -1.3 3,439,583 + +2.0 3,439,583 + -1.0 3,439,583 + -2.0 3,439,583 + -0.3 3,439,583 + -0.3 3,439,583 + -1.6 3,439,583 + -1.3 3,439,583 + +0.0 3,439,584 - +0.4 3,439,584 - -0.6 3,439,584 - -0.6 3,439,584 - +0.0 3,439,584 - -0.3 3,439,584 - +0.8 3,439,584 - +1.0 3,439,609 + +0.7 3,439,610 - -1.4 3,439,623 - +1.0 3,439,643 + +2.0 3,439,643 + +0.4 3,439,643 + -1.3 3,439,644 - +0.4 3,439,710 + OKGIIK_15745 0.37 +1.0 3,439,710 + OKGIIK_15745 0.37 +0.0 3,439,710 + OKGIIK_15745 0.37 +1.7 3,439,710 + OKGIIK_15745 0.37 +1.6 3,439,710 + OKGIIK_15745 0.37 +0.8 3,439,710 + OKGIIK_15745 0.37 -1.0 3,439,710 + OKGIIK_15745 0.37 -0.3 3,439,710 + OKGIIK_15745 0.37 -0.8 3,439,710 + OKGIIK_15745 0.37 -1.1 3,439,710 + OKGIIK_15745 0.37 +1.9 3,439,710 + OKGIIK_15745 0.37 -1.1 3,439,711 - OKGIIK_15745 0.37 -0.2 3,439,711 - OKGIIK_15745 0.37 -0.7 3,439,711 - OKGIIK_15745 0.37 +0.8 3,439,711 - OKGIIK_15745 0.37 -2.3 3,439,711 - OKGIIK_15745 0.37 -1.9 3,439,711 - OKGIIK_15745 0.37 -0.7 3,439,711 - OKGIIK_15745 0.37 -2.0 3,439,711 - OKGIIK_15745 0.37 +0.2 3,439,711 - OKGIIK_15745 0.37 -0.4 3,439,750 - OKGIIK_15745 0.57 -0.6 3,439,852 - -1.3 3,439,852 - -1.8 3,439,852 - +3.0 3,439,852 - +0.0 3,439,852 - -0.6 3,439,852 - -1.0 3,439,852 - +0.6 3,439,852 - +0.2 3,439,852 - +0.8 3,439,852 - -1.6 3,439,852 - -0.8 3,439,852 - -0.7 3,439,852 - +0.0
Or see this region's nucleotide sequence