Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proA and proB overlap by 4 nucleotides proB and OKGIIK_09425 overlap by 4 nucleotides OKGIIK_09425 and argH are separated by 12 nucleotides
OKGIIK_09415: proA - glutamate-5-semialdehyde dehydrogenase, at 2,042,854 to 2,044,116
proA
OKGIIK_09420: proB - glutamate 5-kinase, at 2,044,113 to 2,045,273
proB
OKGIIK_09425: OKGIIK_09425 - Cupin, at 2,045,270 to 2,045,632
_09425
OKGIIK_09430: argH - argininosuccinate lyase, at 2,045,645 to 2,046,940
argH
Position (kb)
2044
2045
2046 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2043.116 kb on + strand, within proA at 2043.197 kb on + strand, within proA at 2043.197 kb on + strand, within proA at 2043.197 kb on + strand, within proA at 2043.197 kb on + strand, within proA at 2043.197 kb on + strand, within proA at 2043.198 kb on - strand, within proA at 2043.198 kb on - strand, within proA at 2043.198 kb on - strand, within proA at 2043.428 kb on + strand, within proA at 2043.428 kb on + strand, within proA at 2043.428 kb on + strand, within proA at 2043.428 kb on + strand, within proA at 2043.428 kb on + strand, within proA at 2043.428 kb on + strand, within proA at 2043.428 kb on + strand, within proA at 2043.428 kb on + strand, within proA at 2043.429 kb on - strand, within proA at 2043.429 kb on - strand, within proA at 2043.429 kb on - strand, within proA at 2043.429 kb on - strand, within proA at 2043.429 kb on - strand, within proA at 2043.429 kb on - strand, within proA at 2043.429 kb on - strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.755 kb on + strand, within proA at 2043.755 kb on + strand, within proA at 2043.755 kb on + strand, within proA at 2043.755 kb on + strand, within proA at 2043.755 kb on + strand, within proA at 2043.755 kb on + strand, within proA at 2043.755 kb on + strand, within proA at 2043.755 kb on + strand, within proA at 2043.756 kb on - strand, within proA at 2043.756 kb on - strand, within proA at 2043.756 kb on - strand, within proA at 2043.756 kb on - strand, within proA at 2043.756 kb on - strand, within proA at 2043.756 kb on - strand, within proA at 2044.057 kb on + strand at 2044.057 kb on + strand at 2044.057 kb on + strand at 2044.057 kb on + strand at 2044.058 kb on - strand at 2044.192 kb on + strand at 2044.192 kb on + strand at 2044.193 kb on - strand at 2044.193 kb on - strand at 2044.193 kb on - strand at 2044.198 kb on + strand at 2044.264 kb on + strand, within proB at 2044.264 kb on + strand, within proB at 2044.264 kb on + strand, within proB at 2044.264 kb on + strand, within proB at 2044.265 kb on - strand, within proB at 2044.265 kb on - strand, within proB at 2044.265 kb on - strand, within proB at 2044.456 kb on + strand, within proB at 2044.456 kb on + strand, within proB at 2044.456 kb on + strand, within proB at 2044.456 kb on + strand, within proB at 2044.456 kb on + strand, within proB at 2044.456 kb on + strand, within proB at 2044.457 kb on - strand, within proB at 2044.457 kb on - strand, within proB at 2044.457 kb on - strand, within proB at 2044.457 kb on - strand, within proB at 2044.457 kb on - strand, within proB at 2044.732 kb on + strand, within proB at 2044.732 kb on + strand, within proB at 2044.732 kb on + strand, within proB at 2044.733 kb on - strand, within proB at 2044.733 kb on - strand, within proB at 2044.733 kb on - strand, within proB at 2044.733 kb on - strand, within proB at 2044.733 kb on - strand, within proB at 2044.733 kb on - strand, within proB at 2044.915 kb on + strand, within proB at 2044.916 kb on - strand, within proB at 2044.916 kb on - strand, within proB at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.470 kb on - strand, within OKGIIK_09425 at 2045.470 kb on - strand, within OKGIIK_09425 at 2045.470 kb on - strand, within OKGIIK_09425 at 2045.470 kb on - strand, within OKGIIK_09425 at 2045.470 kb on - strand, within OKGIIK_09425 at 2045.598 kb on + strand at 2045.598 kb on + strand at 2045.598 kb on + strand at 2045.598 kb on + strand at 2045.598 kb on + strand at 2045.598 kb on + strand at 2045.598 kb on + strand at 2045.599 kb on - strand at 2045.599 kb on - strand at 2045.599 kb on - strand at 2045.665 kb on + strand at 2045.665 kb on + strand at 2045.788 kb on - strand, within argH at 2045.844 kb on + strand, within argH at 2045.844 kb on + strand, within argH at 2045.844 kb on + strand, within argH at 2045.844 kb on + strand, within argH at 2045.844 kb on + strand, within argH at 2045.845 kb on - strand, within argH at 2045.845 kb on - strand, within argH at 2045.845 kb on - strand, within argH at 2045.845 kb on - strand, within argH at 2045.845 kb on - strand, within argH at 2045.845 kb on - strand, within argH at 2045.845 kb on - strand, within argH at 2045.845 kb on - strand, within argH at 2045.845 kb on - strand, within argH at 2045.845 kb on - strand, within argH at 2046.048 kb on + strand, within argH at 2046.048 kb on + strand, within argH at 2046.049 kb on - strand, within argH at 2046.049 kb on - strand, within argH at 2046.049 kb on - strand, within argH at 2046.049 kb on - strand, within argH at 2046.049 kb on - strand, within argH at 2046.117 kb on + strand, within argH at 2046.117 kb on + strand, within argH at 2046.117 kb on + strand, within argH at 2046.117 kb on + strand, within argH at 2046.117 kb on + strand, within argH at 2046.117 kb on + strand, within argH at 2046.117 kb on + strand, within argH at 2046.118 kb on - strand, within argH at 2046.118 kb on - strand, within argH at 2046.118 kb on - strand, within argH at 2046.258 kb on + strand, within argH at 2046.258 kb on + strand, within argH at 2046.259 kb on - strand, within argH at 2046.259 kb on - strand, within argH
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 2,043,116 + proA OKGIIK_09415 0.21 -2.8 2,043,197 + proA OKGIIK_09415 0.27 -1.1 2,043,197 + proA OKGIIK_09415 0.27 -2.2 2,043,197 + proA OKGIIK_09415 0.27 -3.5 2,043,197 + proA OKGIIK_09415 0.27 -3.8 2,043,197 + proA OKGIIK_09415 0.27 +0.1 2,043,198 - proA OKGIIK_09415 0.27 -0.8 2,043,198 - proA OKGIIK_09415 0.27 -4.1 2,043,198 - proA OKGIIK_09415 0.27 -2.2 2,043,428 + proA OKGIIK_09415 0.45 -1.9 2,043,428 + proA OKGIIK_09415 0.45 -1.4 2,043,428 + proA OKGIIK_09415 0.45 -1.9 2,043,428 + proA OKGIIK_09415 0.45 -1.9 2,043,428 + proA OKGIIK_09415 0.45 -1.6 2,043,428 + proA OKGIIK_09415 0.45 -0.6 2,043,428 + proA OKGIIK_09415 0.45 -2.5 2,043,428 + proA OKGIIK_09415 0.45 -0.6 2,043,429 - proA OKGIIK_09415 0.46 -0.5 2,043,429 - proA OKGIIK_09415 0.46 -3.4 2,043,429 - proA OKGIIK_09415 0.46 -1.6 2,043,429 - proA OKGIIK_09415 0.46 -1.9 2,043,429 - proA OKGIIK_09415 0.46 -1.1 2,043,429 - proA OKGIIK_09415 0.46 -3.4 2,043,429 - proA OKGIIK_09415 0.46 -2.4 2,043,449 + proA OKGIIK_09415 0.47 -2.2 2,043,449 + proA OKGIIK_09415 0.47 -1.6 2,043,449 + proA OKGIIK_09415 0.47 -1.1 2,043,449 + proA OKGIIK_09415 0.47 -1.6 2,043,449 + proA OKGIIK_09415 0.47 -3.3 2,043,449 + proA OKGIIK_09415 0.47 +0.6 2,043,449 + proA OKGIIK_09415 0.47 -2.4 2,043,449 + proA OKGIIK_09415 0.47 -3.0 2,043,449 + proA OKGIIK_09415 0.47 -1.4 2,043,449 + proA OKGIIK_09415 0.47 -1.9 2,043,449 + proA OKGIIK_09415 0.47 -2.2 2,043,449 + proA OKGIIK_09415 0.47 -3.0 2,043,449 + proA OKGIIK_09415 0.47 -4.3 2,043,449 + proA OKGIIK_09415 0.47 -2.2 2,043,450 - proA OKGIIK_09415 0.47 -2.4 2,043,450 - proA OKGIIK_09415 0.47 -1.4 2,043,450 - proA OKGIIK_09415 0.47 -0.6 2,043,450 - proA OKGIIK_09415 0.47 -2.1 2,043,450 - proA OKGIIK_09415 0.47 -2.1 2,043,450 - proA OKGIIK_09415 0.47 -1.9 2,043,450 - proA OKGIIK_09415 0.47 -2.4 2,043,450 - proA OKGIIK_09415 0.47 -3.0 2,043,450 - proA OKGIIK_09415 0.47 -1.7 2,043,450 - proA OKGIIK_09415 0.47 -2.1 2,043,450 - proA OKGIIK_09415 0.47 -1.2 2,043,450 - proA OKGIIK_09415 0.47 -2.4 2,043,450 - proA OKGIIK_09415 0.47 -2.4 2,043,755 + proA OKGIIK_09415 0.71 -2.2 2,043,755 + proA OKGIIK_09415 0.71 -1.3 2,043,755 + proA OKGIIK_09415 0.71 -1.1 2,043,755 + proA OKGIIK_09415 0.71 -3.5 2,043,755 + proA OKGIIK_09415 0.71 -3.7 2,043,755 + proA OKGIIK_09415 0.71 -2.4 2,043,755 + proA OKGIIK_09415 0.71 -1.4 2,043,755 + proA OKGIIK_09415 0.71 -1.9 2,043,756 - proA OKGIIK_09415 0.71 -2.9 2,043,756 - proA OKGIIK_09415 0.71 -0.6 2,043,756 - proA OKGIIK_09415 0.71 -2.9 2,043,756 - proA OKGIIK_09415 0.71 -4.4 2,043,756 - proA OKGIIK_09415 0.71 -1.6 2,043,756 - proA OKGIIK_09415 0.71 +0.9 2,044,057 + -2.5 2,044,057 + -1.6 2,044,057 + -2.4 2,044,057 + -3.1 2,044,058 - -0.6 2,044,192 + +0.9 2,044,192 + -0.6 2,044,193 - -1.6 2,044,193 - -0.1 2,044,193 - +0.5 2,044,198 + -1.1 2,044,264 + proB OKGIIK_09420 0.13 -1.4 2,044,264 + proB OKGIIK_09420 0.13 -1.9 2,044,264 + proB OKGIIK_09420 0.13 -2.9 2,044,264 + proB OKGIIK_09420 0.13 -2.6 2,044,265 - proB OKGIIK_09420 0.13 -1.2 2,044,265 - proB OKGIIK_09420 0.13 -1.6 2,044,265 - proB OKGIIK_09420 0.13 -2.6 2,044,456 + proB OKGIIK_09420 0.30 -3.3 2,044,456 + proB OKGIIK_09420 0.30 -0.6 2,044,456 + proB OKGIIK_09420 0.30 -0.1 2,044,456 + proB OKGIIK_09420 0.30 -1.6 2,044,456 + proB OKGIIK_09420 0.30 +0.7 2,044,456 + proB OKGIIK_09420 0.30 -2.2 2,044,457 - proB OKGIIK_09420 0.30 +0.4 2,044,457 - proB OKGIIK_09420 0.30 -1.8 2,044,457 - proB OKGIIK_09420 0.30 -1.9 2,044,457 - proB OKGIIK_09420 0.30 -1.5 2,044,457 - proB OKGIIK_09420 0.30 -0.1 2,044,732 + proB OKGIIK_09420 0.53 +0.9 2,044,732 + proB OKGIIK_09420 0.53 -3.0 2,044,732 + proB OKGIIK_09420 0.53 -1.1 2,044,733 - proB OKGIIK_09420 0.53 +1.5 2,044,733 - proB OKGIIK_09420 0.53 -1.2 2,044,733 - proB OKGIIK_09420 0.53 -0.6 2,044,733 - proB OKGIIK_09420 0.53 -3.1 2,044,733 - proB OKGIIK_09420 0.53 -0.1 2,044,733 - proB OKGIIK_09420 0.53 -0.6 2,044,915 + proB OKGIIK_09420 0.69 -2.1 2,044,916 - proB OKGIIK_09420 0.69 -0.6 2,044,916 - proB OKGIIK_09420 0.69 -1.1 2,045,469 + OKGIIK_09425 0.55 -2.1 2,045,469 + OKGIIK_09425 0.55 -2.2 2,045,469 + OKGIIK_09425 0.55 +0.4 2,045,469 + OKGIIK_09425 0.55 -2.5 2,045,469 + OKGIIK_09425 0.55 -2.8 2,045,469 + OKGIIK_09425 0.55 -1.6 2,045,469 + OKGIIK_09425 0.55 -3.1 2,045,469 + OKGIIK_09425 0.55 -1.3 2,045,469 + OKGIIK_09425 0.55 -2.2 2,045,469 + OKGIIK_09425 0.55 -2.4 2,045,469 + OKGIIK_09425 0.55 -1.1 2,045,470 - OKGIIK_09425 0.55 -1.1 2,045,470 - OKGIIK_09425 0.55 -0.1 2,045,470 - OKGIIK_09425 0.55 -0.9 2,045,470 - OKGIIK_09425 0.55 -1.5 2,045,470 - OKGIIK_09425 0.55 -1.4 2,045,598 + -2.1 2,045,598 + -2.8 2,045,598 + -2.4 2,045,598 + -1.9 2,045,598 + -2.4 2,045,598 + -1.2 2,045,598 + -1.6 2,045,599 - -1.6 2,045,599 - +1.7 2,045,599 - -1.4 2,045,665 + -3.6 2,045,665 + +1.4 2,045,788 - argH OKGIIK_09430 0.11 -0.6 2,045,844 + argH OKGIIK_09430 0.15 -1.1 2,045,844 + argH OKGIIK_09430 0.15 -2.4 2,045,844 + argH OKGIIK_09430 0.15 -2.2 2,045,844 + argH OKGIIK_09430 0.15 -2.6 2,045,844 + argH OKGIIK_09430 0.15 +0.9 2,045,845 - argH OKGIIK_09430 0.15 +0.9 2,045,845 - argH OKGIIK_09430 0.15 -0.1 2,045,845 - argH OKGIIK_09430 0.15 -0.6 2,045,845 - argH OKGIIK_09430 0.15 -2.4 2,045,845 - argH OKGIIK_09430 0.15 +1.9 2,045,845 - argH OKGIIK_09430 0.15 -0.6 2,045,845 - argH OKGIIK_09430 0.15 -1.4 2,045,845 - argH OKGIIK_09430 0.15 +1.9 2,045,845 - argH OKGIIK_09430 0.15 -2.6 2,045,845 - argH OKGIIK_09430 0.15 -0.1 2,046,048 + argH OKGIIK_09430 0.31 -0.9 2,046,048 + argH OKGIIK_09430 0.31 -3.6 2,046,049 - argH OKGIIK_09430 0.31 -0.1 2,046,049 - argH OKGIIK_09430 0.31 -2.1 2,046,049 - argH OKGIIK_09430 0.31 -1.9 2,046,049 - argH OKGIIK_09430 0.31 +0.4 2,046,049 - argH OKGIIK_09430 0.31 -1.6 2,046,117 + argH OKGIIK_09430 0.36 -1.6 2,046,117 + argH OKGIIK_09430 0.36 -1.6 2,046,117 + argH OKGIIK_09430 0.36 -3.1 2,046,117 + argH OKGIIK_09430 0.36 -1.6 2,046,117 + argH OKGIIK_09430 0.36 -0.6 2,046,117 + argH OKGIIK_09430 0.36 -0.1 2,046,117 + argH OKGIIK_09430 0.36 -1.6 2,046,118 - argH OKGIIK_09430 0.36 -1.1 2,046,118 - argH OKGIIK_09430 0.36 -2.1 2,046,118 - argH OKGIIK_09430 0.36 -2.2 2,046,258 + argH OKGIIK_09430 0.47 -1.1 2,046,258 + argH OKGIIK_09430 0.47 -1.4 2,046,259 - argH OKGIIK_09430 0.47 -2.1 2,046,259 - argH OKGIIK_09430 0.47 -1.6
Or see this region's nucleotide sequence