Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_03420 and OKGIIK_03425 are separated by 84 nucleotides OKGIIK_03425 and OKGIIK_03430 are separated by 28 nucleotides OKGIIK_03430 and OKGIIK_03435 are separated by 89 nucleotides OKGIIK_03435 and rlmKL overlap by 4 nucleotides
OKGIIK_03420: OKGIIK_03420 - hypothetical protein, at 763,320 to 763,457
_03420
OKGIIK_03425: OKGIIK_03425 - AraC family transcriptional regulator, at 763,542 to 764,378
_03425
OKGIIK_03430: OKGIIK_03430 - N-acetylmuramoyl-L-alanine amidase, at 764,407 to 764,961
_03430
OKGIIK_03435: OKGIIK_03435 - Alpha/beta hydrolase, at 765,051 to 765,578
_03435
OKGIIK_03440: rlmKL - bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL, at 765,575 to 767,776
rlmKL
Position (kb) 764
765 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 763.426 kb on + strand, within OKGIIK_03420 at 763.535 kb on + strand at 763.536 kb on - strand at 763.536 kb on - strand at 763.536 kb on - strand at 763.594 kb on + strand at 763.594 kb on + strand at 763.594 kb on + strand at 763.594 kb on + strand at 763.595 kb on - strand at 763.595 kb on - strand at 763.720 kb on + strand, within OKGIIK_03425 at 763.726 kb on + strand, within OKGIIK_03425 at 763.726 kb on + strand, within OKGIIK_03425 at 763.726 kb on + strand, within OKGIIK_03425 at 763.726 kb on + strand, within OKGIIK_03425 at 763.727 kb on - strand, within OKGIIK_03425 at 763.727 kb on - strand, within OKGIIK_03425 at 763.727 kb on - strand, within OKGIIK_03425 at 763.727 kb on - strand, within OKGIIK_03425 at 763.768 kb on + strand, within OKGIIK_03425 at 763.768 kb on + strand, within OKGIIK_03425 at 763.768 kb on + strand, within OKGIIK_03425 at 763.768 kb on + strand, within OKGIIK_03425 at 763.768 kb on + strand, within OKGIIK_03425 at 763.768 kb on + strand, within OKGIIK_03425 at 763.768 kb on + strand, within OKGIIK_03425 at 763.769 kb on - strand, within OKGIIK_03425 at 763.769 kb on - strand, within OKGIIK_03425 at 763.769 kb on - strand, within OKGIIK_03425 at 763.769 kb on - strand, within OKGIIK_03425 at 763.769 kb on - strand, within OKGIIK_03425 at 763.769 kb on - strand, within OKGIIK_03425 at 764.161 kb on + strand, within OKGIIK_03425 at 764.162 kb on - strand, within OKGIIK_03425 at 764.236 kb on + strand, within OKGIIK_03425 at 764.236 kb on + strand, within OKGIIK_03425 at 764.237 kb on - strand, within OKGIIK_03425 at 764.237 kb on - strand, within OKGIIK_03425 at 764.237 kb on - strand, within OKGIIK_03425 at 764.237 kb on - strand, within OKGIIK_03425 at 764.237 kb on - strand, within OKGIIK_03425 at 764.237 kb on - strand, within OKGIIK_03425 at 764.237 kb on - strand, within OKGIIK_03425 at 764.237 kb on - strand, within OKGIIK_03425 at 764.237 kb on - strand, within OKGIIK_03425 at 764.408 kb on + strand at 764.408 kb on + strand at 764.408 kb on + strand at 764.408 kb on + strand at 764.408 kb on + strand at 764.408 kb on + strand at 764.409 kb on - strand at 764.670 kb on - strand, within OKGIIK_03430 at 764.753 kb on + strand, within OKGIIK_03430 at 764.754 kb on - strand, within OKGIIK_03430 at 764.783 kb on + strand, within OKGIIK_03430 at 764.783 kb on + strand, within OKGIIK_03430 at 764.783 kb on + strand, within OKGIIK_03430 at 764.783 kb on + strand, within OKGIIK_03430 at 764.783 kb on + strand, within OKGIIK_03430 at 764.783 kb on + strand, within OKGIIK_03430 at 764.783 kb on + strand, within OKGIIK_03430 at 764.783 kb on + strand, within OKGIIK_03430 at 764.783 kb on + strand, within OKGIIK_03430 at 764.783 kb on + strand, within OKGIIK_03430 at 764.783 kb on + strand, within OKGIIK_03430 at 764.783 kb on + strand, within OKGIIK_03430 at 764.784 kb on - strand, within OKGIIK_03430 at 764.784 kb on - strand, within OKGIIK_03430 at 764.784 kb on - strand, within OKGIIK_03430 at 764.784 kb on - strand, within OKGIIK_03430 at 764.784 kb on - strand, within OKGIIK_03430 at 764.784 kb on - strand, within OKGIIK_03430 at 764.784 kb on - strand, within OKGIIK_03430 at 764.784 kb on - strand, within OKGIIK_03430 at 764.784 kb on - strand, within OKGIIK_03430 at 764.784 kb on - strand, within OKGIIK_03430 at 764.784 kb on - strand, within OKGIIK_03430 at 764.784 kb on - strand, within OKGIIK_03430 at 764.786 kb on + strand, within OKGIIK_03430 at 764.786 kb on + strand, within OKGIIK_03430 at 764.786 kb on + strand, within OKGIIK_03430 at 764.786 kb on + strand, within OKGIIK_03430 at 764.786 kb on + strand, within OKGIIK_03430 at 764.786 kb on + strand, within OKGIIK_03430 at 764.786 kb on + strand, within OKGIIK_03430 at 764.786 kb on + strand, within OKGIIK_03430 at 764.786 kb on + strand, within OKGIIK_03430 at 764.787 kb on - strand, within OKGIIK_03430 at 764.834 kb on + strand, within OKGIIK_03430 at 764.928 kb on - strand at 764.928 kb on - strand at 764.928 kb on - strand at 764.928 kb on - strand at 765.008 kb on + strand at 765.008 kb on + strand at 765.008 kb on + strand at 765.008 kb on + strand at 765.009 kb on - strand at 765.009 kb on - strand at 765.009 kb on - strand at 765.009 kb on - strand at 765.009 kb on - strand at 765.009 kb on - strand at 765.009 kb on - strand at 765.165 kb on + strand, within OKGIIK_03435 at 765.165 kb on + strand, within OKGIIK_03435 at 765.166 kb on - strand, within OKGIIK_03435 at 765.166 kb on - strand, within OKGIIK_03435 at 765.363 kb on + strand, within OKGIIK_03435 at 765.524 kb on + strand, within OKGIIK_03435 at 765.524 kb on + strand, within OKGIIK_03435 at 765.524 kb on + strand, within OKGIIK_03435 at 765.524 kb on + strand, within OKGIIK_03435 at 765.524 kb on + strand, within OKGIIK_03435 at 765.524 kb on + strand, within OKGIIK_03435 at 765.524 kb on + strand, within OKGIIK_03435 at 765.524 kb on + strand, within OKGIIK_03435 at 765.524 kb on + strand, within OKGIIK_03435 at 765.525 kb on - strand, within OKGIIK_03435 at 765.525 kb on - strand, within OKGIIK_03435 at 765.525 kb on - strand, within OKGIIK_03435 at 765.525 kb on - strand, within OKGIIK_03435 at 765.525 kb on - strand, within OKGIIK_03435 at 765.525 kb on - strand, within OKGIIK_03435 at 765.629 kb on + strand at 765.629 kb on + strand at 765.629 kb on + strand at 765.629 kb on + strand at 765.629 kb on + strand at 765.629 kb on + strand at 765.629 kb on + strand at 765.629 kb on + strand at 765.629 kb on + strand at 765.629 kb on + strand at 765.630 kb on - strand at 765.630 kb on - strand at 765.630 kb on - strand at 765.630 kb on - strand at 765.630 kb on - strand at 765.630 kb on - strand at 765.630 kb on - strand at 765.630 kb on - strand at 765.630 kb on - strand at 765.630 kb on - strand at 765.630 kb on - strand at 765.630 kb on - strand at 765.722 kb on + strand at 765.722 kb on + strand at 765.722 kb on + strand at 765.723 kb on - strand at 765.723 kb on - strand at 765.723 kb on - strand at 765.723 kb on - strand at 765.723 kb on - strand at 765.723 kb on - strand at 765.723 kb on - strand at 765.806 kb on + strand, within rlmKL at 765.807 kb on - strand, within rlmKL at 765.807 kb on - strand, within rlmKL
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 763,426 + OKGIIK_03420 0.77 -0.5 763,535 + -1.1 763,536 - +0.9 763,536 - +1.9 763,536 - -0.5 763,594 + -0.6 763,594 + +1.4 763,594 + -0.0 763,594 + +0.5 763,595 - -0.7 763,595 - -0.5 763,720 + OKGIIK_03425 0.21 +1.9 763,726 + OKGIIK_03425 0.22 +1.5 763,726 + OKGIIK_03425 0.22 -1.5 763,726 + OKGIIK_03425 0.22 -2.1 763,726 + OKGIIK_03425 0.22 +0.1 763,727 - OKGIIK_03425 0.22 -1.0 763,727 - OKGIIK_03425 0.22 -1.2 763,727 - OKGIIK_03425 0.22 -1.9 763,727 - OKGIIK_03425 0.22 -1.7 763,768 + OKGIIK_03425 0.27 -2.7 763,768 + OKGIIK_03425 0.27 -0.6 763,768 + OKGIIK_03425 0.27 -1.9 763,768 + OKGIIK_03425 0.27 -1.9 763,768 + OKGIIK_03425 0.27 +0.7 763,768 + OKGIIK_03425 0.27 -0.7 763,768 + OKGIIK_03425 0.27 +1.5 763,769 - OKGIIK_03425 0.27 +0.4 763,769 - OKGIIK_03425 0.27 -0.1 763,769 - OKGIIK_03425 0.27 +0.3 763,769 - OKGIIK_03425 0.27 -0.1 763,769 - OKGIIK_03425 0.27 -1.5 763,769 - OKGIIK_03425 0.27 +0.5 764,161 + OKGIIK_03425 0.74 +1.2 764,162 - OKGIIK_03425 0.74 -1.9 764,236 + OKGIIK_03425 0.83 +0.5 764,236 + OKGIIK_03425 0.83 -1.7 764,237 - OKGIIK_03425 0.83 -1.8 764,237 - OKGIIK_03425 0.83 -0.1 764,237 - OKGIIK_03425 0.83 -0.9 764,237 - OKGIIK_03425 0.83 +1.2 764,237 - OKGIIK_03425 0.83 -1.1 764,237 - OKGIIK_03425 0.83 -2.5 764,237 - OKGIIK_03425 0.83 -1.5 764,237 - OKGIIK_03425 0.83 +0.5 764,237 - OKGIIK_03425 0.83 -1.5 764,408 + -0.7 764,408 + -0.2 764,408 + +1.7 764,408 + +1.0 764,408 + -0.6 764,408 + -0.3 764,409 - -1.3 764,670 - OKGIIK_03430 0.47 -0.7 764,753 + OKGIIK_03430 0.62 -0.7 764,754 - OKGIIK_03430 0.63 -2.9 764,783 + OKGIIK_03430 0.68 -0.7 764,783 + OKGIIK_03430 0.68 -1.1 764,783 + OKGIIK_03430 0.68 -3.5 764,783 + OKGIIK_03430 0.68 -1.9 764,783 + OKGIIK_03430 0.68 -1.1 764,783 + OKGIIK_03430 0.68 -0.1 764,783 + OKGIIK_03430 0.68 -1.1 764,783 + OKGIIK_03430 0.68 -1.6 764,783 + OKGIIK_03430 0.68 -2.5 764,783 + OKGIIK_03430 0.68 -2.6 764,783 + OKGIIK_03430 0.68 -0.7 764,783 + OKGIIK_03430 0.68 -2.6 764,784 - OKGIIK_03430 0.68 -2.2 764,784 - OKGIIK_03430 0.68 -1.1 764,784 - OKGIIK_03430 0.68 -1.9 764,784 - OKGIIK_03430 0.68 -1.7 764,784 - OKGIIK_03430 0.68 -0.4 764,784 - OKGIIK_03430 0.68 -0.9 764,784 - OKGIIK_03430 0.68 -0.7 764,784 - OKGIIK_03430 0.68 -3.9 764,784 - OKGIIK_03430 0.68 -1.1 764,784 - OKGIIK_03430 0.68 -0.7 764,784 - OKGIIK_03430 0.68 -1.1 764,784 - OKGIIK_03430 0.68 -1.4 764,786 + OKGIIK_03430 0.68 -1.7 764,786 + OKGIIK_03430 0.68 -0.1 764,786 + OKGIIK_03430 0.68 -1.7 764,786 + OKGIIK_03430 0.68 -2.1 764,786 + OKGIIK_03430 0.68 -0.7 764,786 + OKGIIK_03430 0.68 -1.9 764,786 + OKGIIK_03430 0.68 -2.3 764,786 + OKGIIK_03430 0.68 -2.1 764,786 + OKGIIK_03430 0.68 -2.6 764,787 - OKGIIK_03430 0.68 -2.1 764,834 + OKGIIK_03430 0.77 -0.7 764,928 - -0.6 764,928 - -2.9 764,928 - -1.4 764,928 - -0.7 765,008 + +1.5 765,008 + -0.9 765,008 + -0.7 765,008 + -0.1 765,009 - +1.7 765,009 - +1.9 765,009 - -2.1 765,009 - -2.7 765,009 - -0.5 765,009 - -1.7 765,009 - +1.3 765,165 + OKGIIK_03435 0.22 +0.9 765,165 + OKGIIK_03435 0.22 +0.7 765,166 - OKGIIK_03435 0.22 +0.9 765,166 - OKGIIK_03435 0.22 -1.9 765,363 + OKGIIK_03435 0.59 -2.4 765,524 + OKGIIK_03435 0.90 +0.6 765,524 + OKGIIK_03435 0.90 -0.1 765,524 + OKGIIK_03435 0.90 +1.9 765,524 + OKGIIK_03435 0.90 +0.5 765,524 + OKGIIK_03435 0.90 -0.4 765,524 + OKGIIK_03435 0.90 -0.7 765,524 + OKGIIK_03435 0.90 +0.1 765,524 + OKGIIK_03435 0.90 -0.6 765,524 + OKGIIK_03435 0.90 -0.7 765,525 - OKGIIK_03435 0.90 -0.4 765,525 - OKGIIK_03435 0.90 +0.7 765,525 - OKGIIK_03435 0.90 +0.4 765,525 - OKGIIK_03435 0.90 -1.7 765,525 - OKGIIK_03435 0.90 +0.9 765,525 - OKGIIK_03435 0.90 -0.2 765,629 + -0.1 765,629 + -0.4 765,629 + +1.0 765,629 + +1.5 765,629 + -1.4 765,629 + -2.0 765,629 + -1.1 765,629 + +1.9 765,629 + +0.2 765,629 + -1.0 765,630 - -0.7 765,630 - -1.1 765,630 - +0.2 765,630 - -0.7 765,630 - -0.1 765,630 - +1.3 765,630 - -0.2 765,630 - -0.4 765,630 - -1.3 765,630 - +0.3 765,630 - -1.1 765,630 - +1.6 765,722 + -1.1 765,722 + +0.1 765,722 + -0.3 765,723 - -1.8 765,723 - -1.2 765,723 - -0.7 765,723 - +0.2 765,723 - -1.6 765,723 - -1.5 765,723 - +0.9 765,806 + rlmKL OKGIIK_03440 0.10 -0.1 765,807 - rlmKL OKGIIK_03440 0.11 +3.5 765,807 - rlmKL OKGIIK_03440 0.11 -0.9
Or see this region's nucleotide sequence