Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvC and ruvA are separated by 25 nucleotides ruvA and kup are separated by 2 nucleotides kup and ruvB are separated by 118 nucleotides
OKGIIK_01835: ruvC - crossover junction endodeoxyribonuclease RuvC, at 426,446 to 426,967
ruvC
OKGIIK_01840: ruvA - Holliday junction branch migration protein RuvA, at 426,993 to 427,589
ruvA
OKGIIK_01845: kup - potassium uptake protein, at 427,592 to 429,493
kup
OKGIIK_01850: ruvB - Holliday junction branch migration DNA helicase RuvB, at 429,612 to 430,661
ruvB
Position (kb) 427
428
429
430 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 427.098 kb on + strand, within ruvA at 427.098 kb on + strand, within ruvA at 427.099 kb on - strand, within ruvA at 427.706 kb on + strand at 427.707 kb on + strand at 427.817 kb on + strand, within kup at 427.817 kb on + strand, within kup at 427.818 kb on - strand, within kup at 427.818 kb on - strand, within kup at 427.818 kb on - strand, within kup at 428.045 kb on + strand, within kup at 428.045 kb on + strand, within kup at 428.045 kb on + strand, within kup at 428.046 kb on - strand, within kup at 428.046 kb on - strand, within kup at 428.046 kb on - strand, within kup at 428.315 kb on + strand, within kup at 428.316 kb on - strand, within kup at 428.396 kb on + strand, within kup at 428.396 kb on + strand, within kup at 428.397 kb on - strand, within kup at 428.453 kb on + strand, within kup at 428.453 kb on + strand, within kup at 428.481 kb on - strand, within kup at 428.599 kb on - strand, within kup at 429.319 kb on + strand at 429.319 kb on + strand at 429.319 kb on + strand at 429.320 kb on - strand at 429.504 kb on + strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.543 kb on + strand at 429.543 kb on + strand at 429.544 kb on - strand at 429.578 kb on + strand at 429.578 kb on + strand at 429.578 kb on + strand at 429.578 kb on + strand at 429.578 kb on + strand at 429.578 kb on + strand at 429.578 kb on + strand at 429.578 kb on + strand at 429.578 kb on + strand at 429.579 kb on - strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.585 kb on - strand at 429.585 kb on - strand at 429.585 kb on - strand at 429.585 kb on - strand at 429.585 kb on - strand at 429.585 kb on - strand at 430.159 kb on - strand, within ruvB
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 427,098 + ruvA OKGIIK_01840 0.18 -1.5 427,098 + ruvA OKGIIK_01840 0.18 -1.0 427,099 - ruvA OKGIIK_01840 0.18 +1.0 427,706 + -1.0 427,707 + +0.4 427,817 + kup OKGIIK_01845 0.12 -0.0 427,817 + kup OKGIIK_01845 0.12 -2.0 427,818 - kup OKGIIK_01845 0.12 -0.7 427,818 - kup OKGIIK_01845 0.12 -0.0 427,818 - kup OKGIIK_01845 0.12 -0.0 428,045 + kup OKGIIK_01845 0.24 -1.0 428,045 + kup OKGIIK_01845 0.24 -1.6 428,045 + kup OKGIIK_01845 0.24 +0.7 428,046 - kup OKGIIK_01845 0.24 -1.0 428,046 - kup OKGIIK_01845 0.24 -1.6 428,046 - kup OKGIIK_01845 0.24 -1.0 428,315 + kup OKGIIK_01845 0.38 -1.6 428,316 - kup OKGIIK_01845 0.38 -1.4 428,396 + kup OKGIIK_01845 0.42 +2.0 428,396 + kup OKGIIK_01845 0.42 +1.0 428,397 - kup OKGIIK_01845 0.42 +1.0 428,453 + kup OKGIIK_01845 0.45 -2.4 428,453 + kup OKGIIK_01845 0.45 -1.6 428,481 - kup OKGIIK_01845 0.47 +1.0 428,599 - kup OKGIIK_01845 0.53 -1.8 429,319 + -0.8 429,319 + -1.2 429,319 + +0.2 429,320 - -1.0 429,504 + +1.4 429,505 - +0.6 429,505 - +0.3 429,505 - -0.4 429,505 - +0.1 429,505 - -2.4 429,505 - -1.8 429,505 - -1.2 429,505 - +3.3 429,505 - -1.3 429,505 - -0.3 429,541 + +0.6 429,541 + -1.4 429,541 + +2.3 429,541 + -1.1 429,541 + -1.6 429,541 + -1.0 429,541 + +0.1 429,541 + -0.0 429,541 + +0.5 429,541 + +0.3 429,541 + +1.3 429,541 + -0.6 429,541 + +1.6 429,541 + -0.6 429,541 + -0.4 429,541 + -2.6 429,541 + +0.4 429,541 + +0.2 429,541 + -0.2 429,541 + -0.6 429,541 + -0.0 429,541 + +1.0 429,541 + -2.4 429,541 + +0.2 429,542 - -1.4 429,542 - +1.7 429,542 - -2.0 429,542 - +0.5 429,542 - -0.4 429,542 - +0.2 429,542 - +1.5 429,542 - +0.4 429,542 - -0.1 429,542 - -2.0 429,542 - -1.4 429,542 - +1.0 429,542 - +1.7 429,542 - -1.4 429,542 - +1.0 429,542 - +1.4 429,542 - -2.6 429,542 - -0.8 429,542 - -1.6 429,542 - -2.0 429,542 - -0.0 429,542 - +0.5 429,542 - +1.0 429,542 - -2.0 429,542 - +0.1 429,542 - -0.0 429,542 - -1.0 429,542 - -2.4 429,542 - +0.5 429,542 - -2.2 429,543 + -1.4 429,543 + -0.5 429,544 - -1.3 429,578 + -1.0 429,578 + +0.8 429,578 + +0.2 429,578 + -0.4 429,578 + -0.7 429,578 + +0.4 429,578 + -1.6 429,578 + +0.6 429,578 + +3.5 429,579 - +2.4 429,584 + -0.5 429,584 + +0.3 429,584 + -0.3 429,584 + +0.5 429,584 + -0.6 429,584 + -0.6 429,584 + -1.0 429,584 + +0.8 429,584 + -0.6 429,584 + +1.2 429,584 + -1.0 429,584 + -1.0 429,584 + -1.4 429,584 + -0.5 429,584 + +0.7 429,584 + -0.2 429,584 + -1.0 429,584 + -1.8 429,584 + +1.0 429,584 + +1.0 429,584 + -1.4 429,584 + -1.0 429,584 + -0.8 429,584 + -0.6 429,584 + +0.0 429,584 + -1.0 429,584 + -0.1 429,584 + +0.2 429,584 + +1.0 429,584 + +0.3 429,584 + -1.1 429,584 + -1.6 429,584 + +0.8 429,584 + -0.8 429,584 + +1.0 429,584 + -1.2 429,584 + -2.0 429,584 + -2.2 429,584 + +0.4 429,584 + +1.4 429,584 + +1.7 429,584 + -1.4 429,584 + -0.2 429,584 + +0.2 429,584 + -0.4 429,584 + -0.3 429,584 + -0.0 429,585 - +0.4 429,585 - +0.8 429,585 - -0.4 429,585 - -0.1 429,585 - -0.5 429,585 - -2.0 430,159 - ruvB OKGIIK_01850 0.52 +0.4
Or see this region's nucleotide sequence