Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acoB and pdhA are separated by 3 nucleotides pdhA and tDO2 are separated by 238 nucleotides
OKGIIK_00750: acoB - alpha-ketoacid dehydrogenase subunit beta, at 166,496 to 167,476
acoB
OKGIIK_00755: pdhA - pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha, at 167,480 to 168,565
pdhA
OKGIIK_00760: tDO2 - Tryptophan 2,3-dioxygenase (vermilion), at 168,804 to 169,652
tDO2
Position (kb) 167
168
169 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 166.497 kb on + strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.629 kb on + strand, within acoB at 166.948 kb on - strand, within acoB at 167.511 kb on + strand at 167.511 kb on + strand at 167.512 kb on - strand at 167.512 kb on - strand at 167.794 kb on - strand, within pdhA at 168.204 kb on + strand, within pdhA at 168.234 kb on + strand, within pdhA at 168.235 kb on - strand, within pdhA at 168.240 kb on + strand, within pdhA at 168.240 kb on + strand, within pdhA at 168.240 kb on + strand, within pdhA at 168.240 kb on + strand, within pdhA at 168.240 kb on + strand, within pdhA at 168.240 kb on + strand, within pdhA at 168.240 kb on + strand, within pdhA at 168.241 kb on - strand, within pdhA at 168.241 kb on - strand, within pdhA at 168.241 kb on - strand, within pdhA at 168.241 kb on - strand, within pdhA at 168.303 kb on + strand, within pdhA at 168.304 kb on - strand, within pdhA at 168.372 kb on + strand, within pdhA at 168.373 kb on - strand, within pdhA at 168.448 kb on - strand, within pdhA at 168.501 kb on - strand at 168.525 kb on + strand at 168.526 kb on - strand at 168.526 kb on - strand at 168.534 kb on + strand at 168.658 kb on - strand at 168.658 kb on - strand at 168.685 kb on + strand at 168.685 kb on + strand at 168.686 kb on - strand at 168.686 kb on - strand at 168.686 kb on - strand at 168.686 kb on - strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.877 kb on - strand at 169.134 kb on + strand, within tDO2 at 169.134 kb on + strand, within tDO2 at 169.134 kb on + strand, within tDO2 at 169.134 kb on + strand, within tDO2 at 169.134 kb on + strand, within tDO2 at 169.134 kb on + strand, within tDO2 at 169.135 kb on - strand, within tDO2 at 169.135 kb on - strand, within tDO2 at 169.135 kb on - strand, within tDO2 at 169.188 kb on + strand, within tDO2 at 169.302 kb on + strand, within tDO2 at 169.302 kb on + strand, within tDO2 at 169.302 kb on + strand, within tDO2 at 169.302 kb on + strand, within tDO2 at 169.302 kb on + strand, within tDO2 at 169.303 kb on - strand, within tDO2 at 169.303 kb on - strand, within tDO2 at 169.303 kb on - strand, within tDO2 at 169.303 kb on - strand, within tDO2 at 169.320 kb on + strand, within tDO2 at 169.320 kb on + strand, within tDO2 at 169.320 kb on + strand, within tDO2 at 169.320 kb on + strand, within tDO2 at 169.320 kb on + strand, within tDO2 at 169.320 kb on + strand, within tDO2 at 169.321 kb on - strand, within tDO2 at 169.321 kb on - strand, within tDO2 at 169.383 kb on + strand, within tDO2 at 169.425 kb on + strand, within tDO2 at 169.425 kb on + strand, within tDO2 at 169.425 kb on + strand, within tDO2 at 169.425 kb on + strand, within tDO2 at 169.425 kb on + strand, within tDO2 at 169.426 kb on - strand, within tDO2 at 169.426 kb on - strand, within tDO2 at 169.426 kb on - strand, within tDO2 at 169.426 kb on - strand, within tDO2 at 169.426 kb on - strand, within tDO2 at 169.426 kb on - strand, within tDO2 at 169.455 kb on + strand, within tDO2 at 169.455 kb on + strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 166,497 + -2.5 166,498 - +1.6 166,498 - -0.8 166,498 - +1.0 166,498 - -1.2 166,498 - +0.3 166,629 + acoB OKGIIK_00750 0.14 -1.3 166,948 - acoB OKGIIK_00750 0.46 -1.3 167,511 + +0.0 167,511 + -2.3 167,512 - -1.1 167,512 - +1.9 167,794 - pdhA OKGIIK_00755 0.29 +1.0 168,204 + pdhA OKGIIK_00755 0.67 +0.3 168,234 + pdhA OKGIIK_00755 0.69 -1.3 168,235 - pdhA OKGIIK_00755 0.70 +0.0 168,240 + pdhA OKGIIK_00755 0.70 +0.0 168,240 + pdhA OKGIIK_00755 0.70 +2.0 168,240 + pdhA OKGIIK_00755 0.70 +1.0 168,240 + pdhA OKGIIK_00755 0.70 -0.6 168,240 + pdhA OKGIIK_00755 0.70 -1.0 168,240 + pdhA OKGIIK_00755 0.70 -1.3 168,240 + pdhA OKGIIK_00755 0.70 +0.0 168,241 - pdhA OKGIIK_00755 0.70 +2.0 168,241 - pdhA OKGIIK_00755 0.70 -1.2 168,241 - pdhA OKGIIK_00755 0.70 -1.3 168,241 - pdhA OKGIIK_00755 0.70 +1.0 168,303 + pdhA OKGIIK_00755 0.76 -1.0 168,304 - pdhA OKGIIK_00755 0.76 +1.0 168,372 + pdhA OKGIIK_00755 0.82 +1.0 168,373 - pdhA OKGIIK_00755 0.82 -1.6 168,448 - pdhA OKGIIK_00755 0.89 -1.6 168,501 - -1.6 168,525 + -1.0 168,526 - -1.0 168,526 - +0.0 168,534 + +0.0 168,658 - +0.5 168,658 - +0.5 168,685 + +0.7 168,685 + -2.2 168,686 - +0.1 168,686 - -1.5 168,686 - +0.4 168,686 - -1.3 168,862 + -1.0 168,862 + -0.7 168,862 + -0.6 168,862 + +1.3 168,862 + -2.5 168,862 + +0.0 168,862 + +1.3 168,862 + -2.0 168,862 + +0.0 168,862 + -0.6 168,862 + +2.8 168,862 + -2.6 168,863 - -1.0 168,863 - -1.5 168,863 - -2.2 168,863 - -0.6 168,863 - +1.1 168,863 - +0.0 168,863 - -1.1 168,863 - -2.3 168,863 - +0.6 168,863 - +0.5 168,863 - -0.6 168,867 + -1.1 168,867 + -0.6 168,867 + -0.3 168,867 + -1.0 168,867 + +0.7 168,867 + -0.3 168,867 + -1.0 168,867 + +0.0 168,867 + +0.5 168,867 + -0.5 168,867 + -0.8 168,867 + -1.3 168,868 - +1.6 168,868 - +0.5 168,868 - +1.0 168,868 - +0.6 168,868 - -1.0 168,868 - -0.1 168,868 - -0.4 168,868 - +1.0 168,868 - +0.0 168,868 - +0.3 168,868 - -1.6 168,868 - -0.6 168,877 - +0.4 169,134 + tDO2 OKGIIK_00760 0.39 +1.0 169,134 + tDO2 OKGIIK_00760 0.39 -0.4 169,134 + tDO2 OKGIIK_00760 0.39 -1.0 169,134 + tDO2 OKGIIK_00760 0.39 -0.7 169,134 + tDO2 OKGIIK_00760 0.39 -0.1 169,134 + tDO2 OKGIIK_00760 0.39 -1.0 169,135 - tDO2 OKGIIK_00760 0.39 +0.1 169,135 - tDO2 OKGIIK_00760 0.39 +0.9 169,135 - tDO2 OKGIIK_00760 0.39 -0.8 169,188 + tDO2 OKGIIK_00760 0.45 +2.0 169,302 + tDO2 OKGIIK_00760 0.59 -1.3 169,302 + tDO2 OKGIIK_00760 0.59 +1.1 169,302 + tDO2 OKGIIK_00760 0.59 +1.0 169,302 + tDO2 OKGIIK_00760 0.59 -0.4 169,302 + tDO2 OKGIIK_00760 0.59 -1.6 169,303 - tDO2 OKGIIK_00760 0.59 +0.0 169,303 - tDO2 OKGIIK_00760 0.59 +2.0 169,303 - tDO2 OKGIIK_00760 0.59 -3.2 169,303 - tDO2 OKGIIK_00760 0.59 +1.0 169,320 + tDO2 OKGIIK_00760 0.61 +1.0 169,320 + tDO2 OKGIIK_00760 0.61 -0.5 169,320 + tDO2 OKGIIK_00760 0.61 -0.5 169,320 + tDO2 OKGIIK_00760 0.61 +2.0 169,320 + tDO2 OKGIIK_00760 0.61 +0.2 169,320 + tDO2 OKGIIK_00760 0.61 -0.4 169,321 - tDO2 OKGIIK_00760 0.61 -0.3 169,321 - tDO2 OKGIIK_00760 0.61 -1.3 169,383 + tDO2 OKGIIK_00760 0.68 -1.6 169,425 + tDO2 OKGIIK_00760 0.73 +1.0 169,425 + tDO2 OKGIIK_00760 0.73 -0.1 169,425 + tDO2 OKGIIK_00760 0.73 -0.6 169,425 + tDO2 OKGIIK_00760 0.73 -0.8 169,425 + tDO2 OKGIIK_00760 0.73 +0.4 169,426 - tDO2 OKGIIK_00760 0.73 +0.4 169,426 - tDO2 OKGIIK_00760 0.73 -2.2 169,426 - tDO2 OKGIIK_00760 0.73 -2.8 169,426 - tDO2 OKGIIK_00760 0.73 -1.3 169,426 - tDO2 OKGIIK_00760 0.73 -0.6 169,426 - tDO2 OKGIIK_00760 0.73 +2.2 169,455 + tDO2 OKGIIK_00760 0.77 +0.9 169,455 + tDO2 OKGIIK_00760 0.77 +0.7 169,456 - tDO2 OKGIIK_00760 0.77 +0.1 169,456 - tDO2 OKGIIK_00760 0.77 +2.0 169,456 - tDO2 OKGIIK_00760 0.77 -0.6 169,456 - tDO2 OKGIIK_00760 0.77 -0.6 169,456 - tDO2 OKGIIK_00760 0.77 -0.1 169,456 - tDO2 OKGIIK_00760 0.77 -1.8 169,456 - tDO2 OKGIIK_00760 0.77 -0.4 169,456 - tDO2 OKGIIK_00760 0.77 +1.5 169,456 - tDO2 OKGIIK_00760 0.77 -1.2
Or see this region's nucleotide sequence