Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_00740 and aceF are separated by 604 nucleotides aceF and acoB are separated by 117 nucleotides
OKGIIK_00740: OKGIIK_00740 - L,D-transpeptidase, at 163,816 to 164,433
_00740
OKGIIK_00745: aceF - branched-chain alpha-keto acid dehydrogenase subunit E2, at 165,038 to 166,378
aceF
OKGIIK_00750: acoB - alpha-ketoacid dehydrogenase subunit beta, at 166,496 to 167,476
acoB
Position (kb) 165
166
167 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.174 kb on + strand, within OKGIIK_00740 at 164.174 kb on + strand, within OKGIIK_00740 at 164.174 kb on + strand, within OKGIIK_00740 at 164.174 kb on + strand, within OKGIIK_00740 at 164.175 kb on - strand, within OKGIIK_00740 at 164.175 kb on - strand, within OKGIIK_00740 at 164.175 kb on - strand, within OKGIIK_00740 at 164.175 kb on - strand, within OKGIIK_00740 at 164.263 kb on + strand, within OKGIIK_00740 at 164.263 kb on + strand, within OKGIIK_00740 at 164.263 kb on + strand, within OKGIIK_00740 at 164.263 kb on + strand, within OKGIIK_00740 at 164.263 kb on + strand, within OKGIIK_00740 at 164.263 kb on + strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.293 kb on + strand, within OKGIIK_00740 at 164.294 kb on - strand, within OKGIIK_00740 at 164.341 kb on + strand, within OKGIIK_00740 at 164.341 kb on + strand, within OKGIIK_00740 at 164.341 kb on + strand, within OKGIIK_00740 at 164.341 kb on + strand, within OKGIIK_00740 at 164.341 kb on + strand, within OKGIIK_00740 at 164.341 kb on + strand, within OKGIIK_00740 at 164.342 kb on - strand, within OKGIIK_00740 at 164.342 kb on - strand, within OKGIIK_00740 at 164.342 kb on - strand, within OKGIIK_00740 at 164.342 kb on - strand, within OKGIIK_00740 at 164.342 kb on - strand, within OKGIIK_00740 at 164.342 kb on - strand, within OKGIIK_00740 at 164.386 kb on + strand at 164.386 kb on + strand at 164.386 kb on + strand at 164.386 kb on + strand at 164.387 kb on - strand at 164.387 kb on - strand at 164.387 kb on - strand at 164.387 kb on - strand at 164.403 kb on + strand at 164.403 kb on + strand at 164.404 kb on - strand at 164.404 kb on - strand at 164.404 kb on - strand at 164.404 kb on - strand at 164.404 kb on - strand at 164.431 kb on + strand at 164.431 kb on + strand at 164.431 kb on + strand at 164.432 kb on - strand at 164.432 kb on - strand at 164.432 kb on - strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.621 kb on - strand at 164.621 kb on - strand at 164.621 kb on - strand at 164.621 kb on - strand at 164.621 kb on - strand at 164.621 kb on - strand at 164.621 kb on - strand at 164.621 kb on - strand at 164.946 kb on + strand at 164.946 kb on + strand at 164.946 kb on + strand at 164.946 kb on + strand at 164.947 kb on - strand at 164.980 kb on + strand at 164.980 kb on + strand at 164.980 kb on + strand at 164.980 kb on + strand at 164.980 kb on + strand at 164.980 kb on + strand at 164.981 kb on - strand at 164.981 kb on - strand at 164.981 kb on - strand at 164.981 kb on - strand at 165.280 kb on + strand, within aceF at 165.281 kb on - strand, within aceF at 165.281 kb on - strand, within aceF at 165.283 kb on - strand, within aceF at 165.926 kb on + strand, within aceF at 166.218 kb on + strand, within aceF at 166.219 kb on - strand, within aceF at 166.356 kb on + strand at 166.357 kb on - strand at 166.398 kb on - strand at 166.398 kb on - strand at 166.398 kb on - strand at 166.398 kb on - strand at 166.398 kb on - strand at 166.398 kb on - strand at 166.451 kb on + strand at 166.451 kb on + strand at 166.475 kb on + strand at 166.475 kb on + strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.497 kb on + strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.629 kb on + strand, within acoB at 166.948 kb on - strand, within acoB
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 164,161 + OKGIIK_00740 0.56 -1.8 164,161 + OKGIIK_00740 0.56 -1.3 164,161 + OKGIIK_00740 0.56 +0.4 164,161 + OKGIIK_00740 0.56 -1.6 164,161 + OKGIIK_00740 0.56 +1.0 164,161 + OKGIIK_00740 0.56 -0.6 164,161 + OKGIIK_00740 0.56 -2.2 164,161 + OKGIIK_00740 0.56 +1.0 164,161 + OKGIIK_00740 0.56 -1.3 164,162 - OKGIIK_00740 0.56 -1.6 164,162 - OKGIIK_00740 0.56 +1.7 164,162 - OKGIIK_00740 0.56 +0.1 164,162 - OKGIIK_00740 0.56 +1.0 164,162 - OKGIIK_00740 0.56 +0.3 164,162 - OKGIIK_00740 0.56 -1.0 164,162 - OKGIIK_00740 0.56 +1.0 164,162 - OKGIIK_00740 0.56 -0.2 164,174 + OKGIIK_00740 0.58 -0.8 164,174 + OKGIIK_00740 0.58 -1.6 164,174 + OKGIIK_00740 0.58 +0.4 164,174 + OKGIIK_00740 0.58 +0.5 164,175 - OKGIIK_00740 0.58 +0.3 164,175 - OKGIIK_00740 0.58 -1.8 164,175 - OKGIIK_00740 0.58 +0.6 164,175 - OKGIIK_00740 0.58 +0.7 164,263 + OKGIIK_00740 0.72 -3.2 164,263 + OKGIIK_00740 0.72 -1.1 164,263 + OKGIIK_00740 0.72 -0.6 164,263 + OKGIIK_00740 0.72 +0.0 164,263 + OKGIIK_00740 0.72 +0.0 164,263 + OKGIIK_00740 0.72 -2.2 164,264 - OKGIIK_00740 0.72 +0.0 164,264 - OKGIIK_00740 0.72 -0.8 164,264 - OKGIIK_00740 0.72 -0.7 164,264 - OKGIIK_00740 0.72 -0.6 164,264 - OKGIIK_00740 0.72 +1.3 164,264 - OKGIIK_00740 0.72 +0.7 164,264 - OKGIIK_00740 0.72 +0.0 164,264 - OKGIIK_00740 0.72 +0.6 164,264 - OKGIIK_00740 0.72 +0.5 164,264 - OKGIIK_00740 0.72 +0.9 164,264 - OKGIIK_00740 0.72 +1.2 164,293 + OKGIIK_00740 0.77 -0.3 164,294 - OKGIIK_00740 0.77 +0.5 164,341 + OKGIIK_00740 0.85 -1.0 164,341 + OKGIIK_00740 0.85 +0.2 164,341 + OKGIIK_00740 0.85 -1.2 164,341 + OKGIIK_00740 0.85 +2.8 164,341 + OKGIIK_00740 0.85 -2.5 164,341 + OKGIIK_00740 0.85 +1.7 164,342 - OKGIIK_00740 0.85 +0.2 164,342 - OKGIIK_00740 0.85 -1.0 164,342 - OKGIIK_00740 0.85 -0.6 164,342 - OKGIIK_00740 0.85 +0.7 164,342 - OKGIIK_00740 0.85 -0.2 164,342 - OKGIIK_00740 0.85 +0.2 164,386 + -0.6 164,386 + +0.2 164,386 + +0.5 164,386 + -0.1 164,387 - -2.6 164,387 - +0.6 164,387 - -0.2 164,387 - +2.0 164,403 + +1.4 164,403 + -0.6 164,404 - +0.7 164,404 - -1.0 164,404 - +1.0 164,404 - +1.0 164,404 - -1.8 164,431 + +1.6 164,431 + +1.4 164,431 + +0.6 164,432 - +0.4 164,432 - -1.8 164,432 - -0.6 164,620 + -0.9 164,620 + +0.2 164,620 + -0.1 164,620 + -2.0 164,620 + -2.3 164,620 + -0.3 164,620 + -1.2 164,620 + -0.1 164,620 + +0.7 164,620 + -0.4 164,620 + -2.2 164,620 + +0.0 164,620 + -0.3 164,621 - +0.5 164,621 - +0.6 164,621 - +0.5 164,621 - +2.4 164,621 - -0.6 164,621 - +0.5 164,621 - -2.7 164,621 - -2.3 164,946 + -2.0 164,946 + -0.5 164,946 + -0.3 164,946 + -0.6 164,947 - +1.6 164,980 + -0.5 164,980 + +0.0 164,980 + -1.3 164,980 + -0.3 164,980 + +1.0 164,980 + -1.2 164,981 - +0.0 164,981 - -1.8 164,981 - -0.3 164,981 - +0.2 165,280 + aceF OKGIIK_00745 0.18 +0.0 165,281 - aceF OKGIIK_00745 0.18 -1.7 165,281 - aceF OKGIIK_00745 0.18 +0.0 165,283 - aceF OKGIIK_00745 0.18 +0.0 165,926 + aceF OKGIIK_00745 0.66 -1.0 166,218 + aceF OKGIIK_00745 0.88 -1.0 166,219 - aceF OKGIIK_00745 0.88 -1.0 166,356 + -1.0 166,357 - -1.0 166,398 - -0.3 166,398 - -0.2 166,398 - -1.0 166,398 - +0.0 166,398 - -0.1 166,398 - +0.0 166,451 + +0.0 166,451 + -1.3 166,475 + -2.0 166,475 + -2.3 166,476 - +0.0 166,476 - +1.0 166,476 - -0.4 166,476 - -0.9 166,476 - -0.1 166,476 - -2.0 166,476 - -0.3 166,476 - -0.7 166,476 - -0.8 166,497 + -2.5 166,498 - +1.6 166,498 - -0.8 166,498 - +1.0 166,498 - -1.2 166,498 - +0.3 166,629 + acoB OKGIIK_00750 0.14 -1.3 166,948 - acoB OKGIIK_00750 0.46 -1.3
Or see this region's nucleotide sequence