Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mltB and OKGIIK_00085 overlap by 4 nucleotides OKGIIK_00085 and OKGIIK_00090 are separated by 74 nucleotides OKGIIK_00090 and ybeD overlap by 1 nucleotides ybeD and lipB are separated by 100 nucleotides
OKGIIK_00080: mltB - lytic murein transglycosylase B, at 18,956 to 19,957
mltB
OKGIIK_00085: OKGIIK_00085 - Endolytic peptidoglycan transglycosylase RlpA, at 19,954 to 20,874
_00085
OKGIIK_00090: OKGIIK_00090 - serine-type D-Ala-D-Ala carboxypeptidase, at 20,949 to 22,202
_00090
OKGIIK_00095: ybeD - DUF493 domain-containing protein, at 22,202 to 22,492
ybeD
OKGIIK_00100: lipB - lipoyl(octanoyl) transferase LipB, at 22,593 to 23,291
lipB
Position (kb) 20
21
22
23 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 19.980 kb on - strand at 20.233 kb on + strand, within OKGIIK_00085 at 20.233 kb on + strand, within OKGIIK_00085 at 20.251 kb on + strand, within OKGIIK_00085 at 20.251 kb on + strand, within OKGIIK_00085 at 20.252 kb on - strand, within OKGIIK_00085 at 20.253 kb on + strand, within OKGIIK_00085 at 20.254 kb on - strand, within OKGIIK_00085 at 20.272 kb on + strand, within OKGIIK_00085 at 20.272 kb on + strand, within OKGIIK_00085 at 20.272 kb on + strand, within OKGIIK_00085 at 20.272 kb on + strand, within OKGIIK_00085 at 20.272 kb on + strand, within OKGIIK_00085 at 20.273 kb on - strand, within OKGIIK_00085 at 20.273 kb on - strand, within OKGIIK_00085 at 20.273 kb on - strand, within OKGIIK_00085 at 20.326 kb on + strand, within OKGIIK_00085 at 20.326 kb on + strand, within OKGIIK_00085 at 20.326 kb on + strand, within OKGIIK_00085 at 20.327 kb on - strand, within OKGIIK_00085 at 20.327 kb on - strand, within OKGIIK_00085 at 20.347 kb on + strand, within OKGIIK_00085 at 20.371 kb on + strand, within OKGIIK_00085 at 20.372 kb on - strand, within OKGIIK_00085 at 20.372 kb on - strand, within OKGIIK_00085 at 20.380 kb on + strand, within OKGIIK_00085 at 20.380 kb on + strand, within OKGIIK_00085 at 20.380 kb on + strand, within OKGIIK_00085 at 20.380 kb on + strand, within OKGIIK_00085 at 20.380 kb on + strand, within OKGIIK_00085 at 20.380 kb on + strand, within OKGIIK_00085 at 20.380 kb on + strand, within OKGIIK_00085 at 20.380 kb on + strand, within OKGIIK_00085 at 20.380 kb on + strand, within OKGIIK_00085 at 20.380 kb on + strand, within OKGIIK_00085 at 20.381 kb on - strand, within OKGIIK_00085 at 20.381 kb on - strand, within OKGIIK_00085 at 20.381 kb on - strand, within OKGIIK_00085 at 20.381 kb on - strand, within OKGIIK_00085 at 20.381 kb on - strand, within OKGIIK_00085 at 20.381 kb on - strand, within OKGIIK_00085 at 20.381 kb on - strand, within OKGIIK_00085 at 20.422 kb on + strand, within OKGIIK_00085 at 20.422 kb on + strand, within OKGIIK_00085 at 20.423 kb on - strand, within OKGIIK_00085 at 20.518 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.650 kb on + strand, within OKGIIK_00085 at 20.651 kb on - strand, within OKGIIK_00085 at 20.651 kb on - strand, within OKGIIK_00085 at 20.651 kb on - strand, within OKGIIK_00085 at 20.651 kb on - strand, within OKGIIK_00085 at 20.651 kb on - strand, within OKGIIK_00085 at 20.651 kb on - strand, within OKGIIK_00085 at 20.651 kb on - strand, within OKGIIK_00085 at 20.651 kb on - strand, within OKGIIK_00085 at 20.651 kb on - strand, within OKGIIK_00085 at 20.651 kb on - strand, within OKGIIK_00085 at 20.651 kb on - strand, within OKGIIK_00085 at 20.651 kb on - strand, within OKGIIK_00085 at 20.651 kb on - strand, within OKGIIK_00085 at 20.651 kb on - strand, within OKGIIK_00085 at 20.651 kb on - strand, within OKGIIK_00085 at 20.867 kb on + strand at 21.120 kb on + strand, within OKGIIK_00090 at 21.355 kb on - strand, within OKGIIK_00090 at 21.451 kb on - strand, within OKGIIK_00090 at 21.519 kb on + strand, within OKGIIK_00090 at 21.828 kb on + strand, within OKGIIK_00090 at 21.828 kb on + strand, within OKGIIK_00090 at 21.955 kb on - strand, within OKGIIK_00090 at 22.348 kb on - strand, within ybeD at 22.348 kb on - strand, within ybeD at 22.348 kb on - strand, within ybeD at 22.348 kb on - strand, within ybeD at 22.436 kb on + strand, within ybeD at 22.436 kb on + strand, within ybeD at 22.437 kb on - strand, within ybeD at 22.437 kb on - strand, within ybeD at 22.481 kb on + strand at 22.546 kb on + strand at 22.546 kb on + strand at 22.546 kb on + strand at 22.546 kb on + strand at 22.546 kb on + strand at 22.546 kb on + strand at 22.546 kb on + strand at 22.547 kb on - strand at 22.547 kb on - strand at 22.547 kb on - strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.849 kb on - strand, within lipB at 22.849 kb on - strand, within lipB at 22.849 kb on - strand, within lipB at 22.849 kb on - strand, within lipB at 22.849 kb on - strand, within lipB at 22.849 kb on - strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.008 kb on - strand, within lipB at 23.008 kb on - strand, within lipB at 23.008 kb on - strand, within lipB at 23.008 kb on - strand, within lipB at 23.008 kb on - strand, within lipB at 23.008 kb on - strand, within lipB at 23.008 kb on - strand, within lipB at 23.008 kb on - strand, within lipB at 23.008 kb on - strand, within lipB at 23.130 kb on + strand, within lipB at 23.130 kb on + strand, within lipB at 23.131 kb on - strand, within lipB at 23.158 kb on + strand, within lipB at 23.158 kb on + strand, within lipB at 23.158 kb on + strand, within lipB at 23.158 kb on + strand, within lipB at 23.158 kb on + strand, within lipB at 23.159 kb on - strand, within lipB at 23.159 kb on - strand, within lipB at 23.159 kb on - strand, within lipB at 23.159 kb on - strand, within lipB at 23.159 kb on - strand, within lipB at 23.159 kb on - strand, within lipB at 23.159 kb on - strand, within lipB
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 19,980 - +0.5 20,233 + OKGIIK_00085 0.30 +1.0 20,233 + OKGIIK_00085 0.30 -1.8 20,251 + OKGIIK_00085 0.32 -1.0 20,251 + OKGIIK_00085 0.32 +0.3 20,252 - OKGIIK_00085 0.32 +1.0 20,253 + OKGIIK_00085 0.32 -0.6 20,254 - OKGIIK_00085 0.33 +2.0 20,272 + OKGIIK_00085 0.35 -2.2 20,272 + OKGIIK_00085 0.35 +1.2 20,272 + OKGIIK_00085 0.35 -1.0 20,272 + OKGIIK_00085 0.35 +0.3 20,272 + OKGIIK_00085 0.35 -1.2 20,273 - OKGIIK_00085 0.35 +1.0 20,273 - OKGIIK_00085 0.35 -0.3 20,273 - OKGIIK_00085 0.35 +0.6 20,326 + OKGIIK_00085 0.40 +1.0 20,326 + OKGIIK_00085 0.40 -2.5 20,326 + OKGIIK_00085 0.40 -3.0 20,327 - OKGIIK_00085 0.40 -2.0 20,327 - OKGIIK_00085 0.40 +1.1 20,347 + OKGIIK_00085 0.43 +1.0 20,371 + OKGIIK_00085 0.45 -1.8 20,372 - OKGIIK_00085 0.45 -0.6 20,372 - OKGIIK_00085 0.45 -0.2 20,380 + OKGIIK_00085 0.46 +1.0 20,380 + OKGIIK_00085 0.46 +0.6 20,380 + OKGIIK_00085 0.46 -1.0 20,380 + OKGIIK_00085 0.46 -0.8 20,380 + OKGIIK_00085 0.46 -1.6 20,380 + OKGIIK_00085 0.46 -1.6 20,380 + OKGIIK_00085 0.46 -0.3 20,380 + OKGIIK_00085 0.46 -0.4 20,380 + OKGIIK_00085 0.46 +0.3 20,380 + OKGIIK_00085 0.46 -1.6 20,381 - OKGIIK_00085 0.46 -1.0 20,381 - OKGIIK_00085 0.46 +0.3 20,381 - OKGIIK_00085 0.46 -1.6 20,381 - OKGIIK_00085 0.46 +1.0 20,381 - OKGIIK_00085 0.46 +0.0 20,381 - OKGIIK_00085 0.46 -1.3 20,381 - OKGIIK_00085 0.46 +0.7 20,422 + OKGIIK_00085 0.51 -1.5 20,422 + OKGIIK_00085 0.51 -1.0 20,423 - OKGIIK_00085 0.51 +1.0 20,518 + OKGIIK_00085 0.61 -0.8 20,650 + OKGIIK_00085 0.76 -0.9 20,650 + OKGIIK_00085 0.76 -0.3 20,650 + OKGIIK_00085 0.76 -0.3 20,650 + OKGIIK_00085 0.76 -0.5 20,650 + OKGIIK_00085 0.76 -0.4 20,650 + OKGIIK_00085 0.76 -0.3 20,650 + OKGIIK_00085 0.76 +0.7 20,650 + OKGIIK_00085 0.76 -2.2 20,650 + OKGIIK_00085 0.76 -1.3 20,650 + OKGIIK_00085 0.76 +2.2 20,650 + OKGIIK_00085 0.76 -0.6 20,650 + OKGIIK_00085 0.76 +0.2 20,650 + OKGIIK_00085 0.76 +1.2 20,650 + OKGIIK_00085 0.76 +0.1 20,650 + OKGIIK_00085 0.76 -0.8 20,650 + OKGIIK_00085 0.76 -2.2 20,650 + OKGIIK_00085 0.76 +0.1 20,650 + OKGIIK_00085 0.76 +2.0 20,650 + OKGIIK_00085 0.76 -0.6 20,651 - OKGIIK_00085 0.76 -2.0 20,651 - OKGIIK_00085 0.76 -1.3 20,651 - OKGIIK_00085 0.76 -1.6 20,651 - OKGIIK_00085 0.76 -1.0 20,651 - OKGIIK_00085 0.76 -1.0 20,651 - OKGIIK_00085 0.76 +0.7 20,651 - OKGIIK_00085 0.76 -1.2 20,651 - OKGIIK_00085 0.76 -0.8 20,651 - OKGIIK_00085 0.76 +0.0 20,651 - OKGIIK_00085 0.76 -1.3 20,651 - OKGIIK_00085 0.76 -1.2 20,651 - OKGIIK_00085 0.76 -0.1 20,651 - OKGIIK_00085 0.76 -0.6 20,651 - OKGIIK_00085 0.76 -0.5 20,651 - OKGIIK_00085 0.76 +1.7 20,867 + +0.8 21,120 + OKGIIK_00090 0.14 -2.3 21,355 - OKGIIK_00090 0.32 -1.3 21,451 - OKGIIK_00090 0.40 -0.8 21,519 + OKGIIK_00090 0.45 -1.3 21,828 + OKGIIK_00090 0.70 -0.8 21,828 + OKGIIK_00090 0.70 -1.8 21,955 - OKGIIK_00090 0.80 -0.2 22,348 - ybeD OKGIIK_00095 0.50 -0.6 22,348 - ybeD OKGIIK_00095 0.50 -1.0 22,348 - ybeD OKGIIK_00095 0.50 +0.0 22,348 - ybeD OKGIIK_00095 0.50 +0.6 22,436 + ybeD OKGIIK_00095 0.80 +1.8 22,436 + ybeD OKGIIK_00095 0.80 +0.4 22,437 - ybeD OKGIIK_00095 0.81 -1.0 22,437 - ybeD OKGIIK_00095 0.81 -1.0 22,481 + -0.2 22,546 + +0.0 22,546 + +0.9 22,546 + +0.0 22,546 + +0.6 22,546 + -2.0 22,546 + +0.2 22,546 + -0.6 22,547 - -1.0 22,547 - +0.6 22,547 - -0.3 22,580 + +1.0 22,580 + +0.1 22,580 + +0.9 22,580 + -0.4 22,580 + +0.2 22,580 + +0.5 22,580 + -1.0 22,580 + +0.5 22,580 + -1.0 22,580 + +0.4 22,580 + -1.8 22,580 + -1.1 22,580 + +1.0 22,580 + -0.6 22,580 + +0.5 22,580 + +0.0 22,580 + -1.6 22,580 + -2.0 22,580 + -1.7 22,580 + -1.6 22,580 + -0.4 22,580 + +0.6 22,580 + +1.0 22,580 + +0.7 22,580 + -0.2 22,580 + +0.5 22,581 - -1.8 22,581 - +0.0 22,581 - -0.8 22,581 - -1.5 22,581 - -1.6 22,581 - +0.0 22,581 - -1.0 22,581 - -1.2 22,581 - -1.8 22,581 - +1.9 22,581 - -1.6 22,581 - -1.0 22,581 - +0.0 22,581 - +0.5 22,848 + lipB OKGIIK_00100 0.36 +0.1 22,848 + lipB OKGIIK_00100 0.36 -1.2 22,848 + lipB OKGIIK_00100 0.36 +0.0 22,848 + lipB OKGIIK_00100 0.36 -1.3 22,848 + lipB OKGIIK_00100 0.36 +0.0 22,848 + lipB OKGIIK_00100 0.36 +0.8 22,848 + lipB OKGIIK_00100 0.36 +1.3 22,848 + lipB OKGIIK_00100 0.36 -1.3 22,848 + lipB OKGIIK_00100 0.36 -2.0 22,848 + lipB OKGIIK_00100 0.36 +0.7 22,848 + lipB OKGIIK_00100 0.36 -0.6 22,849 - lipB OKGIIK_00100 0.37 -0.8 22,849 - lipB OKGIIK_00100 0.37 -1.8 22,849 - lipB OKGIIK_00100 0.37 +0.3 22,849 - lipB OKGIIK_00100 0.37 +1.6 22,849 - lipB OKGIIK_00100 0.37 -0.8 22,849 - lipB OKGIIK_00100 0.37 +3.2 23,007 + lipB OKGIIK_00100 0.59 +1.2 23,007 + lipB OKGIIK_00100 0.59 -2.5 23,007 + lipB OKGIIK_00100 0.59 +0.0 23,007 + lipB OKGIIK_00100 0.59 +0.4 23,007 + lipB OKGIIK_00100 0.59 -0.4 23,007 + lipB OKGIIK_00100 0.59 +1.0 23,007 + lipB OKGIIK_00100 0.59 +2.2 23,007 + lipB OKGIIK_00100 0.59 +0.5 23,007 + lipB OKGIIK_00100 0.59 -0.3 23,007 + lipB OKGIIK_00100 0.59 +1.7 23,007 + lipB OKGIIK_00100 0.59 -0.3 23,007 + lipB OKGIIK_00100 0.59 +1.6 23,007 + lipB OKGIIK_00100 0.59 +2.3 23,008 - lipB OKGIIK_00100 0.59 +1.6 23,008 - lipB OKGIIK_00100 0.59 -0.6 23,008 - lipB OKGIIK_00100 0.59 -1.1 23,008 - lipB OKGIIK_00100 0.59 -0.3 23,008 - lipB OKGIIK_00100 0.59 +2.0 23,008 - lipB OKGIIK_00100 0.59 -0.4 23,008 - lipB OKGIIK_00100 0.59 -0.3 23,008 - lipB OKGIIK_00100 0.59 +0.4 23,008 - lipB OKGIIK_00100 0.59 +0.4 23,130 + lipB OKGIIK_00100 0.77 -0.8 23,130 + lipB OKGIIK_00100 0.77 +0.2 23,131 - lipB OKGIIK_00100 0.77 +1.3 23,158 + lipB OKGIIK_00100 0.81 +0.0 23,158 + lipB OKGIIK_00100 0.81 -0.6 23,158 + lipB OKGIIK_00100 0.81 -1.2 23,158 + lipB OKGIIK_00100 0.81 -0.2 23,158 + lipB OKGIIK_00100 0.81 +1.0 23,159 - lipB OKGIIK_00100 0.81 +0.2 23,159 - lipB OKGIIK_00100 0.81 +2.0 23,159 - lipB OKGIIK_00100 0.81 +0.3 23,159 - lipB OKGIIK_00100 0.81 +0.6 23,159 - lipB OKGIIK_00100 0.81 +0.0 23,159 - lipB OKGIIK_00100 0.81 +0.1 23,159 - lipB OKGIIK_00100 0.81 +0.8
Or see this region's nucleotide sequence