Experiment: WM_Bas36
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisB and hisH overlap by 1 nucleotides hisH and hisA overlap by 1 nucleotides hisA and hisF overlap by 19 nucleotides hisF and hisIE overlap by 7 nucleotides
MCAODC_20435: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 893,423 to 894,490
hisB
MCAODC_20440: hisH - imidazole glycerol phosphate synthase subunit HisH, at 894,490 to 895,080
hisH
MCAODC_20445: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 895,080 to 895,817
hisA
MCAODC_20450: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 895,799 to 896,575
hisF
MCAODC_20455: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 896,569 to 897,180
hisIE
Position (kb) 895
896 Strain fitness (log2 ratio) -2
-1
0
1
2 at 894.263 kb on + strand, within hisB at 894.265 kb on + strand, within hisB at 894.266 kb on - strand, within hisB at 894.341 kb on + strand, within hisB at 894.341 kb on + strand, within hisB at 894.342 kb on - strand, within hisB at 894.379 kb on + strand, within hisB at 894.400 kb on - strand at 894.488 kb on + strand at 894.960 kb on + strand, within hisH at 894.960 kb on + strand, within hisH at 894.960 kb on + strand, within hisH at 894.964 kb on + strand, within hisH at 894.965 kb on - strand, within hisH at 895.007 kb on - strand, within hisH at 895.275 kb on - strand, within hisA at 895.557 kb on + strand, within hisA at 895.636 kb on + strand, within hisA at 895.637 kb on - strand, within hisA at 895.638 kb on + strand, within hisA at 895.639 kb on - strand, within hisA at 895.747 kb on + strand at 895.851 kb on + strand at 895.851 kb on + strand at 895.984 kb on + strand, within hisF at 896.047 kb on + strand, within hisF at 896.133 kb on + strand, within hisF at 896.137 kb on + strand, within hisF at 896.137 kb on + strand, within hisF at 896.170 kb on + strand, within hisF at 896.170 kb on + strand, within hisF at 896.170 kb on + strand, within hisF at 896.216 kb on + strand, within hisF at 896.217 kb on - strand, within hisF at 896.232 kb on - strand, within hisF at 896.511 kb on + strand at 896.511 kb on + strand at 896.529 kb on + strand at 896.537 kb on + strand at 896.537 kb on + strand at 896.569 kb on - strand at 896.674 kb on + strand, within hisIE at 896.676 kb on + strand, within hisIE at 896.676 kb on + strand, within hisIE at 896.693 kb on + strand, within hisIE at 896.796 kb on + strand, within hisIE
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas36 remove 894,263 + hisB MCAODC_20435 0.79 -0.9 894,265 + hisB MCAODC_20435 0.79 +1.3 894,266 - hisB MCAODC_20435 0.79 -0.9 894,341 + hisB MCAODC_20435 0.86 +0.1 894,341 + hisB MCAODC_20435 0.86 +0.6 894,342 - hisB MCAODC_20435 0.86 -0.6 894,379 + hisB MCAODC_20435 0.90 -1.1 894,400 - +0.3 894,488 + -1.2 894,960 + hisH MCAODC_20440 0.80 +0.7 894,960 + hisH MCAODC_20440 0.80 -1.1 894,960 + hisH MCAODC_20440 0.80 +0.8 894,964 + hisH MCAODC_20440 0.80 -0.5 894,965 - hisH MCAODC_20440 0.80 +1.4 895,007 - hisH MCAODC_20440 0.87 +0.8 895,275 - hisA MCAODC_20445 0.26 +1.5 895,557 + hisA MCAODC_20445 0.65 -2.2 895,636 + hisA MCAODC_20445 0.75 -0.5 895,637 - hisA MCAODC_20445 0.75 -1.4 895,638 + hisA MCAODC_20445 0.76 -0.4 895,639 - hisA MCAODC_20445 0.76 -0.1 895,747 + -0.1 895,851 + +1.1 895,851 + +0.8 895,984 + hisF MCAODC_20450 0.24 +0.2 896,047 + hisF MCAODC_20450 0.32 -1.5 896,133 + hisF MCAODC_20450 0.43 +1.3 896,137 + hisF MCAODC_20450 0.44 -0.5 896,137 + hisF MCAODC_20450 0.44 +1.8 896,170 + hisF MCAODC_20450 0.48 -0.2 896,170 + hisF MCAODC_20450 0.48 +0.9 896,170 + hisF MCAODC_20450 0.48 -1.8 896,216 + hisF MCAODC_20450 0.54 +1.2 896,217 - hisF MCAODC_20450 0.54 -1.9 896,232 - hisF MCAODC_20450 0.56 +0.3 896,511 + -1.0 896,511 + -1.4 896,529 + +1.6 896,537 + -1.5 896,537 + +0.5 896,569 - +1.2 896,674 + hisIE MCAODC_20455 0.17 -0.3 896,676 + hisIE MCAODC_20455 0.17 +1.9 896,676 + hisIE MCAODC_20455 0.17 -0.3 896,693 + hisIE MCAODC_20455 0.20 -0.5 896,796 + hisIE MCAODC_20455 0.37 +0.5
Or see this region's nucleotide sequence