Experiment: WM_Bas36
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt waaO and rfaY are separated by 25 nucleotides rfaY and rfaJ are separated by 24 nucleotides
MCAODC_06175: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 1,237,197 to 1,238,204
waaO
MCAODC_06180: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 1,238,230 to 1,238,937
rfaY
MCAODC_06185: rfaJ - glucosyltransferase, at 1,238,962 to 1,239,975
rfaJ
Position (kb)
1238
1239 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1237.282 kb on + strand at 1237.324 kb on + strand, within waaO at 1237.490 kb on - strand, within waaO at 1237.512 kb on + strand, within waaO at 1237.570 kb on + strand, within waaO at 1237.720 kb on + strand, within waaO at 1237.732 kb on - strand, within waaO at 1237.918 kb on - strand, within waaO at 1237.920 kb on - strand, within waaO at 1237.981 kb on + strand, within waaO at 1238.087 kb on - strand, within waaO at 1238.211 kb on - strand at 1238.402 kb on + strand, within rfaY at 1238.673 kb on - strand, within rfaY at 1238.825 kb on - strand, within rfaY at 1238.859 kb on + strand, within rfaY at 1238.996 kb on + strand at 1239.045 kb on + strand at 1239.183 kb on - strand, within rfaJ at 1239.338 kb on + strand, within rfaJ at 1239.344 kb on - strand, within rfaJ at 1239.434 kb on + strand, within rfaJ at 1239.435 kb on - strand, within rfaJ at 1239.441 kb on + strand, within rfaJ at 1239.441 kb on + strand, within rfaJ at 1239.493 kb on + strand, within rfaJ at 1239.514 kb on + strand, within rfaJ at 1239.722 kb on - strand, within rfaJ at 1239.835 kb on - strand, within rfaJ at 1239.837 kb on - strand, within rfaJ at 1239.887 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas36 remove 1,237,282 + -1.2 1,237,324 + waaO MCAODC_06175 0.13 +0.7 1,237,490 - waaO MCAODC_06175 0.29 -1.0 1,237,512 + waaO MCAODC_06175 0.31 -2.0 1,237,570 + waaO MCAODC_06175 0.37 -2.1 1,237,720 + waaO MCAODC_06175 0.52 -1.0 1,237,732 - waaO MCAODC_06175 0.53 -1.9 1,237,918 - waaO MCAODC_06175 0.72 -2.7 1,237,920 - waaO MCAODC_06175 0.72 -0.3 1,237,981 + waaO MCAODC_06175 0.78 -2.2 1,238,087 - waaO MCAODC_06175 0.88 +0.1 1,238,211 - -3.7 1,238,402 + rfaY MCAODC_06180 0.24 -0.2 1,238,673 - rfaY MCAODC_06180 0.63 -2.1 1,238,825 - rfaY MCAODC_06180 0.84 -1.6 1,238,859 + rfaY MCAODC_06180 0.89 -0.8 1,238,996 + -2.0 1,239,045 + -3.0 1,239,183 - rfaJ MCAODC_06185 0.22 -1.9 1,239,338 + rfaJ MCAODC_06185 0.37 -1.1 1,239,344 - rfaJ MCAODC_06185 0.38 -0.2 1,239,434 + rfaJ MCAODC_06185 0.47 -3.2 1,239,435 - rfaJ MCAODC_06185 0.47 -3.6 1,239,441 + rfaJ MCAODC_06185 0.47 -0.8 1,239,441 + rfaJ MCAODC_06185 0.47 -0.7 1,239,493 + rfaJ MCAODC_06185 0.52 -4.1 1,239,514 + rfaJ MCAODC_06185 0.54 +0.2 1,239,722 - rfaJ MCAODC_06185 0.75 -4.6 1,239,835 - rfaJ MCAODC_06185 0.86 -4.4 1,239,837 - rfaJ MCAODC_06185 0.86 -3.2 1,239,887 - -3.2
Or see this region's nucleotide sequence