Strain Fitness in Escherichia coli ECRC101 around MCAODC_06180

Experiment: WM_Bas36

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwaaO and rfaY are separated by 25 nucleotidesrfaY and rfaJ are separated by 24 nucleotides MCAODC_06175: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 1,237,197 to 1,238,204 waaO MCAODC_06180: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 1,238,230 to 1,238,937 rfaY MCAODC_06185: rfaJ - glucosyltransferase, at 1,238,962 to 1,239,975 rfaJ Position (kb) 1238 1239Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1237.282 kb on + strandat 1237.324 kb on + strand, within waaOat 1237.490 kb on - strand, within waaOat 1237.512 kb on + strand, within waaOat 1237.570 kb on + strand, within waaOat 1237.720 kb on + strand, within waaOat 1237.732 kb on - strand, within waaOat 1237.918 kb on - strand, within waaOat 1237.920 kb on - strand, within waaOat 1237.981 kb on + strand, within waaOat 1238.087 kb on - strand, within waaOat 1238.211 kb on - strandat 1238.402 kb on + strand, within rfaYat 1238.673 kb on - strand, within rfaYat 1238.825 kb on - strand, within rfaYat 1238.859 kb on + strand, within rfaYat 1238.996 kb on + strandat 1239.045 kb on + strandat 1239.183 kb on - strand, within rfaJat 1239.338 kb on + strand, within rfaJat 1239.344 kb on - strand, within rfaJat 1239.434 kb on + strand, within rfaJat 1239.435 kb on - strand, within rfaJat 1239.441 kb on + strand, within rfaJat 1239.441 kb on + strand, within rfaJat 1239.493 kb on + strand, within rfaJat 1239.514 kb on + strand, within rfaJat 1239.722 kb on - strand, within rfaJat 1239.835 kb on - strand, within rfaJat 1239.837 kb on - strand, within rfaJat 1239.887 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas36
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1,237,282 + -1.2
1,237,324 + waaO MCAODC_06175 0.13 +0.7
1,237,490 - waaO MCAODC_06175 0.29 -1.0
1,237,512 + waaO MCAODC_06175 0.31 -2.0
1,237,570 + waaO MCAODC_06175 0.37 -2.1
1,237,720 + waaO MCAODC_06175 0.52 -1.0
1,237,732 - waaO MCAODC_06175 0.53 -1.9
1,237,918 - waaO MCAODC_06175 0.72 -2.7
1,237,920 - waaO MCAODC_06175 0.72 -0.3
1,237,981 + waaO MCAODC_06175 0.78 -2.2
1,238,087 - waaO MCAODC_06175 0.88 +0.1
1,238,211 - -3.7
1,238,402 + rfaY MCAODC_06180 0.24 -0.2
1,238,673 - rfaY MCAODC_06180 0.63 -2.1
1,238,825 - rfaY MCAODC_06180 0.84 -1.6
1,238,859 + rfaY MCAODC_06180 0.89 -0.8
1,238,996 + -2.0
1,239,045 + -3.0
1,239,183 - rfaJ MCAODC_06185 0.22 -1.9
1,239,338 + rfaJ MCAODC_06185 0.37 -1.1
1,239,344 - rfaJ MCAODC_06185 0.38 -0.2
1,239,434 + rfaJ MCAODC_06185 0.47 -3.2
1,239,435 - rfaJ MCAODC_06185 0.47 -3.6
1,239,441 + rfaJ MCAODC_06185 0.47 -0.8
1,239,441 + rfaJ MCAODC_06185 0.47 -0.7
1,239,493 + rfaJ MCAODC_06185 0.52 -4.1
1,239,514 + rfaJ MCAODC_06185 0.54 +0.2
1,239,722 - rfaJ MCAODC_06185 0.75 -4.6
1,239,835 - rfaJ MCAODC_06185 0.86 -4.4
1,239,837 - rfaJ MCAODC_06185 0.86 -3.2
1,239,887 - -3.2

Or see this region's nucleotide sequence