Experiment: WM_TP6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolA and tolR are separated by 64 nucleotides tolR and tolQ are separated by 3 nucleotides tolQ and ybgC overlap by 4 nucleotides ybgC and ybgE are separated by 149 nucleotides ybgE and cydX overlap by 1 nucleotides cydX and cydB are separated by 14 nucleotides
MCAODC_26030: tolA - cell envelope integrity protein TolA, at 1,918,675 to 1,919,949
tolA
MCAODC_26035: tolR - colicin uptake protein TolR, at 1,920,014 to 1,920,442
tolR
MCAODC_26040: tolQ - Tol-Pal system protein TolQ, at 1,920,446 to 1,921,138
tolQ
MCAODC_26045: ybgC - tol-pal system-associated acyl-CoA thioesterase, at 1,921,135 to 1,921,539
ybgC
MCAODC_26050: ybgE - cyd operon protein YbgE, at 1,921,689 to 1,921,982
ybgE
MCAODC_26055: cydX - cytochrome bd-I oxidase subunit CydX, at 1,921,982 to 1,922,095
cydX
MCAODC_26060: cydB - cytochrome d ubiquinol oxidase subunit II, at 1,922,110 to 1,923,249
cydB
Position (kb) 1920
1921
1922 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1919.514 kb on + strand, within tolA at 1919.875 kb on + strand at 1919.902 kb on - strand at 1919.906 kb on - strand at 1920.015 kb on + strand at 1920.016 kb on - strand at 1920.016 kb on - strand at 1920.016 kb on - strand at 1920.019 kb on + strand at 1920.033 kb on - strand at 1920.058 kb on + strand, within tolR at 1920.062 kb on + strand, within tolR at 1920.084 kb on - strand, within tolR at 1920.523 kb on - strand, within tolQ at 1920.723 kb on + strand, within tolQ at 1920.888 kb on + strand, within tolQ at 1920.942 kb on + strand, within tolQ at 1920.943 kb on - strand, within tolQ at 1921.143 kb on + strand at 1921.158 kb on + strand at 1921.159 kb on - strand at 1921.254 kb on + strand, within ybgC at 1921.255 kb on - strand, within ybgC at 1921.255 kb on - strand, within ybgC at 1921.255 kb on - strand, within ybgC at 1921.255 kb on - strand, within ybgC at 1921.307 kb on - strand, within ybgC at 1921.307 kb on - strand, within ybgC at 1921.318 kb on - strand, within ybgC at 1921.341 kb on - strand, within ybgC at 1921.341 kb on - strand, within ybgC at 1921.344 kb on - strand, within ybgC at 1921.344 kb on - strand, within ybgC at 1921.359 kb on + strand, within ybgC at 1921.360 kb on - strand, within ybgC at 1921.360 kb on - strand, within ybgC at 1921.446 kb on - strand, within ybgC at 1921.457 kb on + strand, within ybgC at 1921.458 kb on - strand, within ybgC at 1921.499 kb on + strand at 1921.499 kb on + strand at 1921.500 kb on - strand at 1921.503 kb on - strand at 1921.543 kb on - strand at 1921.543 kb on - strand at 1921.543 kb on - strand at 1921.543 kb on - strand at 1921.543 kb on - strand at 1921.587 kb on - strand at 1921.587 kb on - strand at 1921.589 kb on + strand at 1921.590 kb on - strand at 1921.592 kb on - strand at 1921.627 kb on + strand at 1921.665 kb on + strand at 1921.890 kb on - strand, within ybgE at 1921.890 kb on - strand, within ybgE at 1921.890 kb on - strand, within ybgE at 1921.957 kb on + strand at 1921.976 kb on + strand at 1922.108 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP6 remove 1,919,514 + tolA MCAODC_26030 0.66 +1.0 1,919,875 + +1.6 1,919,902 - +3.0 1,919,906 - +0.6 1,920,015 + -1.6 1,920,016 - +1.3 1,920,016 - +1.1 1,920,016 - +0.2 1,920,019 + -1.6 1,920,033 - -0.2 1,920,058 + tolR MCAODC_26035 0.10 -0.8 1,920,062 + tolR MCAODC_26035 0.11 +2.0 1,920,084 - tolR MCAODC_26035 0.16 +1.0 1,920,523 - tolQ MCAODC_26040 0.11 +1.0 1,920,723 + tolQ MCAODC_26040 0.40 -0.1 1,920,888 + tolQ MCAODC_26040 0.64 +0.6 1,920,942 + tolQ MCAODC_26040 0.72 -1.7 1,920,943 - tolQ MCAODC_26040 0.72 -1.2 1,921,143 + -1.4 1,921,158 + -1.0 1,921,159 - +0.6 1,921,254 + ybgC MCAODC_26045 0.29 +1.0 1,921,255 - ybgC MCAODC_26045 0.30 -0.1 1,921,255 - ybgC MCAODC_26045 0.30 -1.6 1,921,255 - ybgC MCAODC_26045 0.30 +1.6 1,921,255 - ybgC MCAODC_26045 0.30 +0.9 1,921,307 - ybgC MCAODC_26045 0.42 -1.2 1,921,307 - ybgC MCAODC_26045 0.42 +1.4 1,921,318 - ybgC MCAODC_26045 0.45 -0.9 1,921,341 - ybgC MCAODC_26045 0.51 +0.0 1,921,341 - ybgC MCAODC_26045 0.51 +0.6 1,921,344 - ybgC MCAODC_26045 0.52 +0.7 1,921,344 - ybgC MCAODC_26045 0.52 +0.8 1,921,359 + ybgC MCAODC_26045 0.55 -2.1 1,921,360 - ybgC MCAODC_26045 0.56 +2.1 1,921,360 - ybgC MCAODC_26045 0.56 +1.1 1,921,446 - ybgC MCAODC_26045 0.77 -0.1 1,921,457 + ybgC MCAODC_26045 0.80 -1.6 1,921,458 - ybgC MCAODC_26045 0.80 +0.3 1,921,499 + -1.2 1,921,499 + -1.0 1,921,500 - +0.4 1,921,503 - -0.1 1,921,543 - +0.5 1,921,543 - -0.4 1,921,543 - -1.5 1,921,543 - +0.3 1,921,543 - -0.5 1,921,587 - +0.3 1,921,587 - +0.9 1,921,589 + -0.2 1,921,590 - +0.8 1,921,592 - -0.0 1,921,627 + +3.4 1,921,665 + +2.0 1,921,890 - ybgE MCAODC_26050 0.68 +0.8 1,921,890 - ybgE MCAODC_26050 0.68 -1.5 1,921,890 - ybgE MCAODC_26050 0.68 -0.1 1,921,957 + +1.0 1,921,976 + -0.4 1,922,108 - +0.8
Or see this region's nucleotide sequence