Strain Fitness in Escherichia coli ECRC101 around MCAODC_18770

Experiment: WM_TP6

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdgcP and yoaJ are separated by 133 nucleotidesyoaJ and yeaQ are separated by 58 nucleotidesyeaQ and yoaG are separated by 146 nucleotidesyoaG and yeaR are separated by 3 nucleotidesyeaR and leuE are separated by 172 nucleotidesleuE and dmlR are separated by 126 nucleotides MCAODC_18750: dgcP - diguanylate cyclase DgcP, at 608,588 to 609,613 dgcP MCAODC_18755: yoaJ - protein YoaJ, at 609,747 to 609,821 yoaJ MCAODC_18760: yeaQ - UPF0410 protein YeaQ, at 609,880 to 610,128 yeaQ MCAODC_18765: yoaG - Protein YoaG, at 610,275 to 610,457 yoaG MCAODC_18770: yeaR - Uncharacterized protein YeaR, at 610,461 to 610,820 yeaR MCAODC_18775: leuE - leucine efflux protein LeuE, at 610,993 to 611,631 leuE MCAODC_18780: dmlR - DNA-binding transcriptional regulator DmlR, at 611,758 to 612,681 dmlR Position (kb) 610 611Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 609.489 kb on + strand, within dgcPat 609.529 kb on - strandat 609.674 kb on + strandat 609.675 kb on - strandat 609.684 kb on + strandat 609.743 kb on - strandat 609.767 kb on + strand, within yoaJat 609.785 kb on + strand, within yoaJat 609.819 kb on + strandat 609.889 kb on - strandat 609.908 kb on - strand, within yeaQat 609.908 kb on - strand, within yeaQat 609.908 kb on - strand, within yeaQat 610.060 kb on - strand, within yeaQat 610.183 kb on - strandat 610.273 kb on - strandat 610.276 kb on + strandat 610.277 kb on - strandat 610.313 kb on - strand, within yoaGat 610.313 kb on - strand, within yoaGat 610.441 kb on + strandat 610.441 kb on + strandat 610.442 kb on - strandat 610.544 kb on - strand, within yeaRat 610.548 kb on - strand, within yeaRat 610.548 kb on - strand, within yeaRat 610.658 kb on - strand, within yeaRat 611.083 kb on - strand, within leuEat 611.086 kb on + strand, within leuEat 611.086 kb on + strand, within leuEat 611.086 kb on + strand, within leuEat 611.087 kb on - strand, within leuEat 611.087 kb on - strand, within leuEat 611.116 kb on - strand, within leuEat 611.133 kb on - strand, within leuEat 611.215 kb on + strand, within leuEat 611.231 kb on + strand, within leuEat 611.479 kb on - strand, within leuEat 611.483 kb on - strand, within leuEat 611.502 kb on - strand, within leuEat 611.669 kb on - strandat 611.733 kb on - strandat 611.733 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP6
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609,489 + dgcP MCAODC_18750 0.88 -0.3
609,529 - -1.4
609,674 + +0.5
609,675 - -0.4
609,684 + -0.9
609,743 - +0.4
609,767 + yoaJ MCAODC_18755 0.27 -0.6
609,785 + yoaJ MCAODC_18755 0.51 +1.0
609,819 + -0.4
609,889 - -0.7
609,908 - yeaQ MCAODC_18760 0.11 -0.5
609,908 - yeaQ MCAODC_18760 0.11 +1.3
609,908 - yeaQ MCAODC_18760 0.11 -1.3
610,060 - yeaQ MCAODC_18760 0.72 +1.6
610,183 - -1.5
610,273 - -0.7
610,276 + +1.2
610,277 - +0.2
610,313 - yoaG MCAODC_18765 0.21 -0.4
610,313 - yoaG MCAODC_18765 0.21 -1.8
610,441 + -0.2
610,441 + -1.0
610,442 - -1.3
610,544 - yeaR MCAODC_18770 0.23 -1.0
610,548 - yeaR MCAODC_18770 0.24 -0.5
610,548 - yeaR MCAODC_18770 0.24 -1.4
610,658 - yeaR MCAODC_18770 0.55 -0.3
611,083 - leuE MCAODC_18775 0.14 +0.3
611,086 + leuE MCAODC_18775 0.15 +0.0
611,086 + leuE MCAODC_18775 0.15 -0.4
611,086 + leuE MCAODC_18775 0.15 +0.6
611,087 - leuE MCAODC_18775 0.15 +2.2
611,087 - leuE MCAODC_18775 0.15 +2.0
611,116 - leuE MCAODC_18775 0.19 +1.2
611,133 - leuE MCAODC_18775 0.22 +3.9
611,215 + leuE MCAODC_18775 0.35 -0.9
611,231 + leuE MCAODC_18775 0.37 +0.7
611,479 - leuE MCAODC_18775 0.76 +0.2
611,483 - leuE MCAODC_18775 0.77 -1.6
611,502 - leuE MCAODC_18775 0.80 -1.2
611,669 - +1.1
611,733 - -0.3
611,733 - +1.1

Or see this region's nucleotide sequence