Strain Fitness in Escherichia coli ECRC101 around MCAODC_16225

Experiment: WM_TP6

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_16210 and MCAODC_16215 are separated by 4 nucleotidesMCAODC_16215 and MCAODC_16220 overlap by 14 nucleotidesMCAODC_16220 and MCAODC_16225 are separated by 124 nucleotidesMCAODC_16225 and ydcF are separated by 115 nucleotides MCAODC_16210: MCAODC_16210 - hypothetical protein, at 140,502 to 140,927 _16210 MCAODC_16215: MCAODC_16215 - hypothetical protein, at 140,932 to 141,270 _16215 MCAODC_16220: MCAODC_16220 - hypothetical protein, at 141,257 to 141,688 _16220 MCAODC_16225: MCAODC_16225 - hypothetical protein, at 141,813 to 142,190 _16225 MCAODC_16230: ydcF - Protein YdcF, at 142,306 to 143,106 ydcF Position (kb) 141 142 143Strain fitness (log2 ratio) -2 -1 0 1 2at 141.797 kb on + strandat 141.947 kb on - strand, within MCAODC_16225at 142.001 kb on + strand, within MCAODC_16225at 142.001 kb on + strand, within MCAODC_16225at 142.076 kb on + strand, within MCAODC_16225at 142.077 kb on - strand, within MCAODC_16225at 142.119 kb on + strand, within MCAODC_16225at 142.119 kb on + strand, within MCAODC_16225at 142.178 kb on - strandat 142.182 kb on + strandat 142.219 kb on + strandat 142.262 kb on + strandat 142.265 kb on + strandat 142.266 kb on - strandat 142.266 kb on - strandat 142.266 kb on - strandat 142.346 kb on + strandat 142.346 kb on + strandat 142.347 kb on - strandat 142.427 kb on + strand, within ydcFat 142.470 kb on + strand, within ydcFat 142.567 kb on + strand, within ydcFat 142.701 kb on + strand, within ydcFat 142.747 kb on - strand, within ydcFat 142.823 kb on + strand, within ydcFat 142.862 kb on + strand, within ydcFat 142.884 kb on - strand, within ydcFat 142.890 kb on + strand, within ydcFat 142.901 kb on + strand, within ydcFat 142.927 kb on + strand, within ydcFat 143.100 kb on - strandat 143.183 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP6
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141,797 + -0.7
141,947 - MCAODC_16225 0.35 +2.3
142,001 + MCAODC_16225 0.50 -0.3
142,001 + MCAODC_16225 0.50 -0.6
142,076 + MCAODC_16225 0.70 -0.1
142,077 - MCAODC_16225 0.70 -0.8
142,119 + MCAODC_16225 0.81 -1.8
142,119 + MCAODC_16225 0.81 +1.0
142,178 - -0.5
142,182 + -0.8
142,219 + +0.4
142,262 + -1.2
142,265 + -0.3
142,266 - +1.4
142,266 - +0.9
142,266 - +1.1
142,346 + +0.0
142,346 + +0.3
142,347 - +0.8
142,427 + ydcF MCAODC_16230 0.15 +0.9
142,470 + ydcF MCAODC_16230 0.20 -0.3
142,567 + ydcF MCAODC_16230 0.33 -0.5
142,701 + ydcF MCAODC_16230 0.49 +2.7
142,747 - ydcF MCAODC_16230 0.55 +1.0
142,823 + ydcF MCAODC_16230 0.65 -0.3
142,862 + ydcF MCAODC_16230 0.69 +1.7
142,884 - ydcF MCAODC_16230 0.72 +0.6
142,890 + ydcF MCAODC_16230 0.73 -0.5
142,901 + ydcF MCAODC_16230 0.74 +0.6
142,927 + ydcF MCAODC_16230 0.78 -0.0
143,100 - -0.4
143,183 - +2.3

Or see this region's nucleotide sequence