Experiment: WM_TP6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt puuD and puuR are separated by 26 nucleotides puuR and puuC are separated by 149 nucleotides puuC and puuB are separated by 1 nucleotides
MCAODC_15830: puuD - gamma-glutamyl-gamma-aminobutyrate hydrolase, at 59,041 to 59,805
puuD
MCAODC_15835: puuR - HTH-type transcriptional regulator PuuR, at 59,832 to 60,389
puuR
MCAODC_15840: puuC - aldehyde dehydrogenase PuuC, at 60,539 to 62,026
puuC
MCAODC_15845: puuB - gamma-glutamylputrescine oxidase, at 62,028 to 63,308
puuB
Position (kb) 60
61
62
63 Strain fitness (log2 ratio) -2
-1
0
1
2 at 59.651 kb on + strand, within puuD at 59.651 kb on + strand, within puuD at 59.742 kb on + strand at 59.781 kb on + strand at 59.818 kb on - strand at 59.950 kb on + strand, within puuR at 59.981 kb on + strand, within puuR at 59.982 kb on - strand, within puuR at 60.007 kb on - strand, within puuR at 60.071 kb on + strand, within puuR at 60.171 kb on + strand, within puuR at 60.203 kb on + strand, within puuR at 60.204 kb on - strand, within puuR at 60.223 kb on + strand, within puuR at 60.223 kb on + strand, within puuR at 60.248 kb on + strand, within puuR at 60.297 kb on + strand, within puuR at 60.305 kb on + strand, within puuR at 60.305 kb on + strand, within puuR at 60.404 kb on - strand at 60.508 kb on + strand at 60.509 kb on - strand at 60.509 kb on - strand at 60.510 kb on + strand at 60.511 kb on - strand at 60.511 kb on - strand at 60.511 kb on - strand at 60.579 kb on + strand at 60.620 kb on + strand at 60.620 kb on + strand at 60.620 kb on + strand at 60.899 kb on - strand, within puuC at 60.953 kb on + strand, within puuC at 60.953 kb on + strand, within puuC at 60.954 kb on - strand, within puuC at 61.698 kb on + strand, within puuC at 62.039 kb on + strand at 62.040 kb on - strand at 62.040 kb on - strand at 62.040 kb on - strand at 62.049 kb on + strand at 62.052 kb on + strand at 62.052 kb on + strand at 62.069 kb on + strand at 62.357 kb on + strand, within puuB at 62.420 kb on + strand, within puuB at 62.568 kb on - strand, within puuB at 62.632 kb on - strand, within puuB at 62.685 kb on - strand, within puuB at 62.712 kb on + strand, within puuB at 62.713 kb on - strand, within puuB at 62.853 kb on + strand, within puuB at 62.871 kb on - strand, within puuB at 62.938 kb on - strand, within puuB
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP6 remove 59,651 + puuD MCAODC_15830 0.80 -0.7 59,651 + puuD MCAODC_15830 0.80 +1.0 59,742 + +2.7 59,781 + -0.2 59,818 - -0.1 59,950 + puuR MCAODC_15835 0.21 +0.3 59,981 + puuR MCAODC_15835 0.27 -1.2 59,982 - puuR MCAODC_15835 0.27 -0.0 60,007 - puuR MCAODC_15835 0.31 -0.0 60,071 + puuR MCAODC_15835 0.43 -0.5 60,171 + puuR MCAODC_15835 0.61 -0.1 60,203 + puuR MCAODC_15835 0.66 -1.0 60,204 - puuR MCAODC_15835 0.67 -1.1 60,223 + puuR MCAODC_15835 0.70 -0.3 60,223 + puuR MCAODC_15835 0.70 -0.8 60,248 + puuR MCAODC_15835 0.75 +1.1 60,297 + puuR MCAODC_15835 0.83 +2.1 60,305 + puuR MCAODC_15835 0.85 +1.3 60,305 + puuR MCAODC_15835 0.85 -2.7 60,404 - -0.8 60,508 + +0.8 60,509 - +2.7 60,509 - +0.7 60,510 + -0.7 60,511 - -0.6 60,511 - +0.1 60,511 - +0.6 60,579 + +0.6 60,620 + +0.4 60,620 + +0.7 60,620 + +2.7 60,899 - puuC MCAODC_15840 0.24 -0.2 60,953 + puuC MCAODC_15840 0.28 -0.0 60,953 + puuC MCAODC_15840 0.28 -1.0 60,954 - puuC MCAODC_15840 0.28 -1.4 61,698 + puuC MCAODC_15840 0.78 -1.2 62,039 + -0.4 62,040 - +0.7 62,040 - +1.4 62,040 - -1.3 62,049 + +0.1 62,052 + +0.3 62,052 + -0.4 62,069 + +0.8 62,357 + puuB MCAODC_15845 0.26 +0.9 62,420 + puuB MCAODC_15845 0.31 +1.4 62,568 - puuB MCAODC_15845 0.42 +0.7 62,632 - puuB MCAODC_15845 0.47 -1.4 62,685 - puuB MCAODC_15845 0.51 -1.0 62,712 + puuB MCAODC_15845 0.53 +1.2 62,713 - puuB MCAODC_15845 0.53 -0.1 62,853 + puuB MCAODC_15845 0.64 -0.1 62,871 - puuB MCAODC_15845 0.66 -1.4 62,938 - puuB MCAODC_15845 0.71 +0.6
Or see this region's nucleotide sequence