Strain Fitness in Escherichia coli ECRC101 around MCAODC_15705

Experiment: WM_TP6

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpgpB and lapA are separated by 148 nucleotideslapA and lapB are separated by 6 nucleotides MCAODC_15700: pgpB - phosphatidylglycerophosphatase B, at 30,024 to 30,788 pgpB MCAODC_15705: lapA - lipopolysaccharide assembly protein LapA, at 30,937 to 31,245 lapA MCAODC_15710: lapB - lipopolysaccharide assembly protein LapB, at 31,252 to 32,421 lapB Position (kb) 30 31 32Strain fitness (log2 ratio) -2 -1 0 1 2 3at 30.007 kb on - strandat 30.074 kb on + strandat 30.136 kb on + strand, within pgpBat 30.257 kb on - strand, within pgpBat 30.299 kb on + strand, within pgpBat 30.394 kb on + strand, within pgpBat 30.459 kb on + strand, within pgpBat 30.587 kb on + strand, within pgpBat 30.664 kb on + strand, within pgpBat 30.668 kb on + strand, within pgpBat 30.668 kb on + strand, within pgpBat 30.668 kb on + strand, within pgpBat 30.669 kb on - strand, within pgpBat 30.734 kb on - strandat 30.763 kb on + strandat 30.763 kb on + strandat 30.874 kb on + strandat 30.874 kb on + strandat 30.882 kb on + strandat 30.901 kb on + strandat 30.947 kb on + strandat 31.026 kb on + strand, within lapAat 31.030 kb on + strand, within lapAat 31.056 kb on + strand, within lapAat 31.169 kb on + strand, within lapA

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP6
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30,007 - -0.9
30,074 + +0.1
30,136 + pgpB MCAODC_15700 0.15 +3.5
30,257 - pgpB MCAODC_15700 0.30 +0.5
30,299 + pgpB MCAODC_15700 0.36 +0.7
30,394 + pgpB MCAODC_15700 0.48 -2.2
30,459 + pgpB MCAODC_15700 0.57 +0.9
30,587 + pgpB MCAODC_15700 0.74 +0.9
30,664 + pgpB MCAODC_15700 0.84 -0.2
30,668 + pgpB MCAODC_15700 0.84 +0.8
30,668 + pgpB MCAODC_15700 0.84 +0.6
30,668 + pgpB MCAODC_15700 0.84 +0.5
30,669 - pgpB MCAODC_15700 0.84 -0.2
30,734 - +0.2
30,763 + -1.1
30,763 + +0.5
30,874 + +1.3
30,874 + +0.4
30,882 + +1.3
30,901 + -1.1
30,947 + -1.0
31,026 + lapA MCAODC_15705 0.29 -1.6
31,030 + lapA MCAODC_15705 0.30 +0.5
31,056 + lapA MCAODC_15705 0.39 -1.2
31,169 + lapA MCAODC_15705 0.75 -0.9

Or see this region's nucleotide sequence