Experiment: WM_TP6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnp-IS3 and MCAODC_14870 are separated by 54 nucleotides MCAODC_14870 and renD overlap by 4 nucleotides renD and emrE are separated by 67 nucleotides emrE and ybcK are separated by 256 nucleotides
MCAODC_14865: tnp-IS3 - IS3 family transposase, at 2,917,025 to 2,917,351
tnp-IS3
MCAODC_14870: MCAODC_14870 - replication protein P, at 2,917,406 to 2,917,843
_14870
MCAODC_14875: renD - Putative protein RenD, at 2,917,840 to 2,918,142
renD
MCAODC_14880: emrE - multidrug efflux SMR transporter EmrE, at 2,918,210 to 2,918,542
emrE
MCAODC_14885: ybcK - Uncharacterized protein YbcK, at 2,918,799 to 2,920,325
ybcK
Position (kb) 2918
2919 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 2917.526 kb on - strand, within MCAODC_14870 at 2917.526 kb on - strand, within MCAODC_14870 at 2918.222 kb on - strand at 2918.422 kb on + strand, within emrE at 2918.427 kb on + strand, within emrE at 2918.474 kb on - strand, within emrE at 2918.476 kb on - strand, within emrE
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP6 remove 2,917,526 - MCAODC_14870 0.27 -0.1 2,917,526 - MCAODC_14870 0.27 +0.9 2,918,222 - +3.3 2,918,422 + emrE MCAODC_14880 0.64 -2.2 2,918,427 + emrE MCAODC_14880 0.65 -2.8 2,918,474 - emrE MCAODC_14880 0.79 +0.0 2,918,476 - emrE MCAODC_14880 0.80 -1.2
Or see this region's nucleotide sequence