Experiment: WM_TP6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fMet_trna and rimP are separated by 206 nucleotides rimP and nusA are separated by 27 nucleotides nusA and infB are separated by 24 nucleotides
MCAODC_08615: fMet_trna - tRNA-fMet(cat), at 1,725,408 to 1,725,484
_trna
MCAODC_08620: rimP - ribosome maturation factor RimP, at 1,725,691 to 1,726,143
rimP
MCAODC_08625: nusA - Transcription termination/antitermination protein NusA, at 1,726,171 to 1,727,658
nusA
MCAODC_08630: infB - Translation initiation factor IF-2, at 1,727,683 to 1,730,355
infB
Position (kb)
1726
1727
1728 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1725.179 kb on - strand at 1725.197 kb on + strand at 1725.207 kb on - strand at 1725.233 kb on - strand at 1725.310 kb on + strand at 1725.310 kb on + strand at 1725.313 kb on - strand at 1725.328 kb on + strand at 1725.328 kb on + strand at 1725.361 kb on + strand at 1725.512 kb on + strand at 1725.601 kb on + strand at 1725.605 kb on + strand at 1727.451 kb on + strand, within nusA at 1727.489 kb on + strand, within nusA at 1727.532 kb on + strand at 1727.532 kb on + strand at 1727.532 kb on + strand at 1727.628 kb on + strand at 1727.666 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP6 remove 1,725,179 - +1.1 1,725,197 + -0.5 1,725,207 - +0.2 1,725,233 - +1.2 1,725,310 + -0.9 1,725,310 + -1.4 1,725,313 - +0.2 1,725,328 + -0.3 1,725,328 + +1.5 1,725,361 + +0.7 1,725,512 + -0.5 1,725,601 + +4.0 1,725,605 + -0.8 1,727,451 + nusA MCAODC_08625 0.86 -2.9 1,727,489 + nusA MCAODC_08625 0.89 -0.4 1,727,532 + +0.4 1,727,532 + -0.7 1,727,532 + -0.6 1,727,628 + +0.4 1,727,666 + +1.8
Or see this region's nucleotide sequence