Experiment: WM_TP6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt spoT and rpoZ are separated by 18 nucleotides rpoZ and gmk are separated by 54 nucleotides gmk and ligB are separated by 257 nucleotides
MCAODC_06065: spoT - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 1,217,801 to 1,219,909
spoT
MCAODC_06070: rpoZ - DNA-directed RNA polymerase subunit omega, at 1,219,928 to 1,220,203
rpoZ
MCAODC_06075: gmk - guanylate kinase, at 1,220,258 to 1,220,881
gmk
MCAODC_06080: ligB - NAD-dependent DNA ligase LigB, at 1,221,139 to 1,222,821
ligB
Position (kb)
1219
1220
1221 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1220.145 kb on - strand, within rpoZ at 1220.175 kb on - strand, within rpoZ at 1220.193 kb on - strand at 1220.208 kb on - strand at 1220.208 kb on - strand at 1220.208 kb on - strand at 1220.208 kb on - strand at 1220.895 kb on - strand at 1220.895 kb on - strand at 1220.895 kb on - strand at 1220.918 kb on - strand at 1220.918 kb on - strand at 1220.922 kb on - strand at 1220.922 kb on - strand at 1220.922 kb on - strand at 1220.922 kb on - strand at 1220.922 kb on - strand at 1220.922 kb on - strand at 1220.957 kb on - strand at 1220.957 kb on - strand at 1220.957 kb on - strand at 1221.000 kb on + strand at 1221.028 kb on - strand at 1221.028 kb on - strand at 1221.035 kb on + strand at 1221.105 kb on + strand at 1221.105 kb on + strand at 1221.105 kb on + strand at 1221.105 kb on + strand at 1221.105 kb on + strand at 1221.105 kb on + strand at 1221.105 kb on + strand at 1221.105 kb on + strand at 1221.105 kb on + strand at 1221.105 kb on + strand at 1221.105 kb on + strand at 1221.105 kb on + strand at 1221.105 kb on + strand at 1221.106 kb on - strand at 1221.106 kb on - strand at 1221.106 kb on - strand at 1221.106 kb on - strand at 1221.106 kb on - strand at 1221.106 kb on - strand at 1221.106 kb on - strand at 1221.106 kb on - strand at 1221.106 kb on - strand at 1221.106 kb on - strand at 1221.107 kb on + strand at 1221.107 kb on + strand at 1221.107 kb on + strand at 1221.107 kb on + strand at 1221.146 kb on + strand at 1221.146 kb on + strand at 1221.147 kb on - strand at 1221.147 kb on - strand at 1221.161 kb on + strand at 1221.161 kb on + strand at 1221.161 kb on + strand at 1221.161 kb on + strand at 1221.162 kb on - strand at 1221.162 kb on - strand at 1221.162 kb on - strand at 1221.162 kb on - strand at 1221.162 kb on - strand at 1221.164 kb on + strand at 1221.164 kb on + strand at 1221.164 kb on + strand at 1221.164 kb on + strand at 1221.164 kb on + strand at 1221.164 kb on + strand at 1221.165 kb on - strand at 1221.165 kb on - strand at 1221.165 kb on - strand at 1221.165 kb on - strand at 1221.165 kb on - strand at 1221.165 kb on - strand at 1221.165 kb on - strand at 1221.165 kb on - strand at 1221.165 kb on - strand at 1221.165 kb on - strand at 1221.165 kb on - strand at 1221.168 kb on + strand at 1221.169 kb on - strand at 1221.169 kb on - strand at 1221.169 kb on - strand at 1221.169 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP6 remove 1,220,145 - rpoZ MCAODC_06070 0.79 -0.6 1,220,175 - rpoZ MCAODC_06070 0.89 -1.1 1,220,193 - -2.5 1,220,208 - +0.4 1,220,208 - -0.2 1,220,208 - -1.2 1,220,208 - -3.2 1,220,895 - -0.5 1,220,895 - +0.2 1,220,895 - -0.4 1,220,918 - -1.8 1,220,918 - -0.7 1,220,922 - -1.1 1,220,922 - +0.1 1,220,922 - -0.2 1,220,922 - +0.9 1,220,922 - +0.6 1,220,922 - +0.2 1,220,957 - -0.8 1,220,957 - +1.1 1,220,957 - -0.1 1,221,000 + +0.6 1,221,028 - +0.8 1,221,028 - -0.2 1,221,035 + -1.5 1,221,105 + -0.8 1,221,105 + +0.2 1,221,105 + -0.1 1,221,105 + +0.3 1,221,105 + +0.5 1,221,105 + -1.8 1,221,105 + -1.2 1,221,105 + -0.2 1,221,105 + +0.6 1,221,105 + -1.2 1,221,105 + +0.4 1,221,105 + -0.6 1,221,105 + +0.9 1,221,106 - +1.4 1,221,106 - +0.3 1,221,106 - +0.4 1,221,106 - -0.4 1,221,106 - -0.1 1,221,106 - -1.7 1,221,106 - -0.0 1,221,106 - +0.6 1,221,106 - +2.1 1,221,106 - +0.2 1,221,107 + +0.4 1,221,107 + +0.9 1,221,107 + +0.1 1,221,107 + -0.2 1,221,146 + +0.8 1,221,146 + +0.3 1,221,147 - +0.0 1,221,147 - +0.0 1,221,161 + -1.3 1,221,161 + +0.1 1,221,161 + +0.5 1,221,161 + +3.0 1,221,162 - -0.1 1,221,162 - -0.1 1,221,162 - -2.3 1,221,162 - -0.6 1,221,162 - +0.5 1,221,164 + +2.4 1,221,164 + +0.2 1,221,164 + -0.9 1,221,164 + +0.8 1,221,164 + -0.4 1,221,164 + -0.8 1,221,165 - -0.4 1,221,165 - +0.1 1,221,165 - -0.9 1,221,165 - +0.8 1,221,165 - -1.0 1,221,165 - +0.7 1,221,165 - -1.5 1,221,165 - -0.1 1,221,165 - +0.3 1,221,165 - -1.2 1,221,165 - -0.2 1,221,168 + +0.2 1,221,169 - +4.1 1,221,169 - -0.5 1,221,169 - +0.3 1,221,169 - +0.3
Or see this region's nucleotide sequence