Experiment: WM_TP6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt surA and pdxA overlap by 1 nucleotides pdxA and rsmA overlap by 4 nucleotides rsmA and apaG are separated by 2 nucleotides apaG and apaH are separated by 6 nucleotides
MCAODC_01340: surA - peptidylprolyl isomerase SurA, at 257,518 to 258,804
surA
MCAODC_01345: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 258,804 to 259,793
pdxA
MCAODC_01350: rsmA - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA, at 259,790 to 260,611
rsmA
MCAODC_01355: apaG - Co2+/Mg2+ efflux protein ApaG, at 260,614 to 260,991
apaG
MCAODC_01360: apaH - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH, at 260,998 to 261,846
apaH
Position (kb) 259
260
261 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 258.803 kb on - strand at 258.803 kb on - strand at 258.803 kb on - strand at 258.865 kb on + strand at 258.865 kb on + strand at 258.865 kb on + strand at 258.865 kb on + strand at 258.866 kb on - strand at 258.866 kb on - strand at 258.979 kb on - strand, within pdxA at 258.979 kb on - strand, within pdxA at 258.979 kb on - strand, within pdxA at 259.011 kb on - strand, within pdxA at 259.027 kb on + strand, within pdxA at 259.028 kb on - strand, within pdxA at 259.028 kb on - strand, within pdxA at 259.028 kb on - strand, within pdxA at 259.070 kb on - strand, within pdxA at 259.089 kb on + strand, within pdxA at 259.090 kb on - strand, within pdxA at 259.090 kb on - strand, within pdxA at 259.212 kb on - strand, within pdxA at 259.328 kb on + strand, within pdxA at 259.329 kb on - strand, within pdxA at 259.362 kb on - strand, within pdxA at 259.394 kb on + strand, within pdxA at 259.455 kb on - strand, within pdxA at 259.471 kb on + strand, within pdxA at 259.471 kb on + strand, within pdxA at 259.472 kb on - strand, within pdxA at 259.472 kb on - strand, within pdxA at 259.481 kb on + strand, within pdxA at 259.482 kb on - strand, within pdxA at 259.482 kb on - strand, within pdxA at 259.482 kb on - strand, within pdxA at 259.563 kb on + strand, within pdxA at 259.564 kb on - strand, within pdxA at 259.597 kb on - strand, within pdxA at 259.597 kb on - strand, within pdxA at 259.597 kb on - strand, within pdxA at 259.655 kb on + strand, within pdxA at 259.655 kb on + strand, within pdxA at 259.655 kb on + strand, within pdxA at 259.818 kb on + strand at 260.135 kb on + strand, within rsmA at 260.137 kb on + strand, within rsmA at 260.137 kb on + strand, within rsmA at 260.138 kb on - strand, within rsmA at 260.170 kb on + strand, within rsmA at 260.174 kb on + strand, within rsmA at 260.288 kb on + strand, within rsmA at 260.467 kb on + strand, within rsmA at 260.576 kb on + strand at 260.637 kb on + strand at 260.657 kb on - strand, within apaG at 260.692 kb on + strand, within apaG at 260.704 kb on + strand, within apaG at 260.705 kb on - strand, within apaG at 260.705 kb on - strand, within apaG at 260.706 kb on + strand, within apaG at 260.706 kb on + strand, within apaG at 260.717 kb on + strand, within apaG at 260.857 kb on + strand, within apaG at 260.858 kb on - strand, within apaG at 261.007 kb on + strand at 261.007 kb on + strand at 261.007 kb on + strand at 261.007 kb on + strand at 261.008 kb on - strand at 261.008 kb on - strand at 261.173 kb on + strand, within apaH at 261.173 kb on + strand, within apaH at 261.174 kb on - strand, within apaH at 261.294 kb on + strand, within apaH at 261.580 kb on + strand, within apaH
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP6 remove 258,803 - +0.7 258,803 - -0.5 258,803 - +2.2 258,865 + -1.8 258,865 + +0.2 258,865 + -0.7 258,865 + +0.3 258,866 - +0.7 258,866 - +0.2 258,979 - pdxA MCAODC_01345 0.18 +0.4 258,979 - pdxA MCAODC_01345 0.18 +1.3 258,979 - pdxA MCAODC_01345 0.18 +0.4 259,011 - pdxA MCAODC_01345 0.21 -0.2 259,027 + pdxA MCAODC_01345 0.23 -1.5 259,028 - pdxA MCAODC_01345 0.23 -0.1 259,028 - pdxA MCAODC_01345 0.23 -0.2 259,028 - pdxA MCAODC_01345 0.23 -0.4 259,070 - pdxA MCAODC_01345 0.27 +0.9 259,089 + pdxA MCAODC_01345 0.29 +0.0 259,090 - pdxA MCAODC_01345 0.29 -1.4 259,090 - pdxA MCAODC_01345 0.29 +0.5 259,212 - pdxA MCAODC_01345 0.41 -2.1 259,328 + pdxA MCAODC_01345 0.53 -0.1 259,329 - pdxA MCAODC_01345 0.53 +0.3 259,362 - pdxA MCAODC_01345 0.56 +1.1 259,394 + pdxA MCAODC_01345 0.60 +1.1 259,455 - pdxA MCAODC_01345 0.66 -1.3 259,471 + pdxA MCAODC_01345 0.67 +0.8 259,471 + pdxA MCAODC_01345 0.67 +0.8 259,472 - pdxA MCAODC_01345 0.67 -1.4 259,472 - pdxA MCAODC_01345 0.67 +0.6 259,481 + pdxA MCAODC_01345 0.68 +0.4 259,482 - pdxA MCAODC_01345 0.68 -1.8 259,482 - pdxA MCAODC_01345 0.68 +0.1 259,482 - pdxA MCAODC_01345 0.68 -0.7 259,563 + pdxA MCAODC_01345 0.77 -1.0 259,564 - pdxA MCAODC_01345 0.77 -0.4 259,597 - pdxA MCAODC_01345 0.80 +0.9 259,597 - pdxA MCAODC_01345 0.80 -1.5 259,597 - pdxA MCAODC_01345 0.80 -2.3 259,655 + pdxA MCAODC_01345 0.86 -0.3 259,655 + pdxA MCAODC_01345 0.86 -0.1 259,655 + pdxA MCAODC_01345 0.86 -0.3 259,818 + +3.9 260,135 + rsmA MCAODC_01350 0.42 +1.6 260,137 + rsmA MCAODC_01350 0.42 +0.7 260,137 + rsmA MCAODC_01350 0.42 +1.1 260,138 - rsmA MCAODC_01350 0.42 +1.4 260,170 + rsmA MCAODC_01350 0.46 +1.1 260,174 + rsmA MCAODC_01350 0.47 +2.3 260,288 + rsmA MCAODC_01350 0.61 -0.1 260,467 + rsmA MCAODC_01350 0.82 -0.3 260,576 + -0.6 260,637 + +0.7 260,657 - apaG MCAODC_01355 0.11 +2.1 260,692 + apaG MCAODC_01355 0.21 -1.5 260,704 + apaG MCAODC_01355 0.24 +0.1 260,705 - apaG MCAODC_01355 0.24 -0.1 260,705 - apaG MCAODC_01355 0.24 -0.8 260,706 + apaG MCAODC_01355 0.24 -1.2 260,706 + apaG MCAODC_01355 0.24 +0.6 260,717 + apaG MCAODC_01355 0.27 +0.6 260,857 + apaG MCAODC_01355 0.64 -0.0 260,858 - apaG MCAODC_01355 0.65 +1.1 261,007 + +0.0 261,007 + +0.3 261,007 + +2.9 261,007 + -0.0 261,008 - +2.3 261,008 - -0.1 261,173 + apaH MCAODC_01360 0.21 +0.1 261,173 + apaH MCAODC_01360 0.21 -0.9 261,174 - apaH MCAODC_01360 0.21 -1.4 261,294 + apaH MCAODC_01360 0.35 +0.3 261,580 + apaH MCAODC_01360 0.69 -1.2
Or see this region's nucleotide sequence