Strain Fitness in Escherichia coli ECRC101 around MCAODC_20485

Experiment: Control_ECRC101_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcpsG and manC2 are separated by 3 nucleotidesmanC2 and wbdQ overlap by 19 nucleotideswbdQ and wcaG are separated by 2 nucleotides MCAODC_20480: cpsG - colanic acid biosynthesis phosphomannomutase CpsG, at 903,350 to 904,720 cpsG MCAODC_20485: manC2 - mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase, at 904,724 to 906,172 manC2 MCAODC_20490: wbdQ - GDP-mannose mannosyl hydrolase WbdQ/WbhG, at 906,154 to 906,663 wbdQ MCAODC_20495: wcaG - GDP-fucose synthetase, at 906,666 to 907,631 wcaG Position (kb) 904 905 906 907Strain fitness (log2 ratio) -2 -1 0 1at 904.235 kb on - strand, within cpsGat 904.237 kb on - strand, within cpsGat 904.411 kb on - strand, within cpsGat 904.721 kb on - strandat 904.760 kb on - strandat 904.760 kb on - strandat 904.760 kb on - strandat 904.780 kb on - strandat 904.861 kb on - strandat 905.072 kb on - strand, within manC2at 905.077 kb on - strand, within manC2at 905.077 kb on - strand, within manC2at 906.176 kb on - strandat 906.350 kb on - strand, within wbdQat 906.534 kb on - strand, within wbdQat 906.991 kb on - strand, within wcaG

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101_WM
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904,235 - cpsG MCAODC_20480 0.65 -1.0
904,237 - cpsG MCAODC_20480 0.65 -1.1
904,411 - cpsG MCAODC_20480 0.77 -1.7
904,721 - +0.9
904,760 - -0.4
904,760 - +0.2
904,760 - +0.0
904,780 - +0.2
904,861 - +1.6
905,072 - manC2 MCAODC_20485 0.24 -2.7
905,077 - manC2 MCAODC_20485 0.24 -1.7
905,077 - manC2 MCAODC_20485 0.24 -0.6
906,176 - +0.5
906,350 - wbdQ MCAODC_20490 0.38 -2.7
906,534 - wbdQ MCAODC_20490 0.75 +0.2
906,991 - wcaG MCAODC_20495 0.34 -1.2

Or see this region's nucleotide sequence