Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvB and ruvA are separated by 8 nucleotides ruvA and yobI are separated by 138 nucleotides yobI and MCAODC_19135 are separated by 70 nucleotides MCAODC_19135 and ruvC are separated by 1 nucleotides ruvC and MCAODC_19145 overlap by 1 nucleotides MCAODC_19145 and MCAODC_19150 overlap by 1 nucleotides
MCAODC_19120: ruvB - Holliday junction branch migration DNA helicase RuvB, at 675,543 to 676,553
ruvB
MCAODC_19125: ruvA - Holliday junction branch migration protein RuvA, at 676,562 to 677,173
ruvA
MCAODC_19130: yobI - Uncharacterized protein YobI, at 677,312 to 677,377
yobI
MCAODC_19135: MCAODC_19135 - Protein, at 677,448 to 678,050
_19135
MCAODC_19140: ruvC - crossover junction endodeoxyribonuclease RuvC, at 678,052 to 678,573
ruvC
MCAODC_19145: MCAODC_19145 - Crossover junction endodeoxyribonuclease RuvC, at 678,573 to 678,608
_19145
MCAODC_19150: MCAODC_19150 - putative transcriptional regulatory protein YebC, at 678,608 to 679,348
_19150
Position (kb) 677
678
679 Strain fitness (log2 ratio) -1
0
1
2 at 676.462 kb on + strand at 676.500 kb on + strand at 677.203 kb on - strand at 677.211 kb on - strand at 677.211 kb on - strand at 677.295 kb on - strand at 677.295 kb on - strand at 677.302 kb on - strand at 677.302 kb on - strand at 677.527 kb on + strand, within MCAODC_19135 at 677.934 kb on - strand, within MCAODC_19135 at 678.046 kb on + strand at 678.046 kb on + strand at 678.047 kb on - strand at 678.049 kb on - strand at 678.115 kb on - strand, within ruvC at 678.472 kb on + strand, within ruvC at 678.652 kb on - strand at 678.728 kb on - strand, within MCAODC_19150 at 678.728 kb on - strand, within MCAODC_19150 at 678.856 kb on - strand, within MCAODC_19150 at 678.856 kb on - strand, within MCAODC_19150
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 676,462 + -0.9 676,500 + +2.2 677,203 - -0.5 677,211 - +0.3 677,211 - +2.1 677,295 - +0.1 677,295 - +0.0 677,302 - -1.4 677,302 - +0.2 677,527 + MCAODC_19135 0.13 -1.7 677,934 - MCAODC_19135 0.81 -1.5 678,046 + -0.0 678,046 + -0.9 678,047 - +0.3 678,049 - -0.6 678,115 - ruvC MCAODC_19140 0.12 +0.0 678,472 + ruvC MCAODC_19140 0.80 -1.6 678,652 - +0.5 678,728 - MCAODC_19150 0.16 +0.6 678,728 - MCAODC_19150 0.16 -0.5 678,856 - MCAODC_19150 0.33 +0.2 678,856 - MCAODC_19150 0.33 +0.3
Or see this region's nucleotide sequence