Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt escU and etgA overlap by 4 nucleotides etgA and grlR are separated by 195 nucleotides grlR and grlA are separated by 54 nucleotides grlA and cesD are separated by 383 nucleotides
MCAODC_05815: escU - type III secretion system LEE export apparatus switch protein EscU, at 1,169,877 to 1,170,914
escU
MCAODC_05820: etgA - type III secretion system LEE muramidase EtgA, at 1,170,911 to 1,171,369
etgA
MCAODC_05825: grlR - type III secretion system LEE GrlA-binding negative regulator GrlR, at 1,171,565 to 1,171,936
grlR
MCAODC_05830: grlA - type III secretion system LEE transcriptional regulator GrlA, at 1,171,991 to 1,172,404
grlA
MCAODC_05835: cesD - SycD/LcrH family type III secretion system chaperone CesD, at 1,172,788 to 1,173,243
cesD
Position (kb)
1171
1172 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1170.626 kb on + strand, within escU at 1170.630 kb on - strand, within escU at 1170.686 kb on - strand, within escU at 1170.690 kb on + strand, within escU at 1170.692 kb on + strand, within escU at 1170.712 kb on + strand, within escU at 1170.836 kb on + strand at 1170.846 kb on + strand at 1171.003 kb on + strand, within etgA at 1171.183 kb on + strand, within etgA at 1171.195 kb on - strand, within etgA at 1171.281 kb on - strand, within etgA at 1171.547 kb on + strand at 1171.570 kb on + strand at 1171.828 kb on - strand, within grlR at 1172.045 kb on + strand, within grlA at 1172.238 kb on - strand, within grlA at 1172.240 kb on - strand, within grlA at 1172.533 kb on + strand at 1172.566 kb on + strand at 1172.579 kb on - strand at 1172.656 kb on + strand at 1172.933 kb on + strand, within cesD
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 1,170,626 + escU MCAODC_05815 0.72 +2.1 1,170,630 - escU MCAODC_05815 0.73 -0.2 1,170,686 - escU MCAODC_05815 0.78 -0.2 1,170,690 + escU MCAODC_05815 0.78 -0.2 1,170,692 + escU MCAODC_05815 0.79 -1.0 1,170,712 + escU MCAODC_05815 0.80 +0.0 1,170,836 + +0.2 1,170,846 + -0.6 1,171,003 + etgA MCAODC_05820 0.20 -1.9 1,171,183 + etgA MCAODC_05820 0.59 +0.5 1,171,195 - etgA MCAODC_05820 0.62 -0.7 1,171,281 - etgA MCAODC_05820 0.81 -0.4 1,171,547 + -0.6 1,171,570 + -0.4 1,171,828 - grlR MCAODC_05825 0.71 -1.0 1,172,045 + grlA MCAODC_05830 0.13 -0.2 1,172,238 - grlA MCAODC_05830 0.60 -0.3 1,172,240 - grlA MCAODC_05830 0.60 +1.4 1,172,533 + +0.5 1,172,566 + -0.2 1,172,579 - -0.3 1,172,656 + -0.1 1,172,933 + cesD MCAODC_05835 0.32 -1.3
Or see this region's nucleotide sequence