Experiment: WM_EV219
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ureD1 and MCAODC_23655 are separated by 380 nucleotides MCAODC_23655 and opgE are separated by 224 nucleotides opgE and dgkA are separated by 92 nucleotides
MCAODC_23650: ureD1 - Putative urease accessory UreD-like protein, at 1,449,291 to 1,449,989
ureD1
MCAODC_23655: MCAODC_23655 - hypothetical protein, at 1,450,370 to 1,450,645
_23655
MCAODC_23660: opgE - phosphoethanolamine transferase, at 1,450,870 to 1,452,489
opgE
MCAODC_23665: dgkA - Diacylglycerol kinase, at 1,452,582 to 1,452,941
dgkA
Position (kb)
1450
1451
1452
1453 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1450.092 kb on - strand at 1450.093 kb on + strand at 1450.095 kb on + strand at 1450.095 kb on + strand at 1450.181 kb on + strand at 1450.181 kb on + strand at 1450.181 kb on + strand at 1450.181 kb on + strand at 1450.182 kb on - strand at 1450.182 kb on - strand at 1450.204 kb on + strand at 1450.260 kb on - strand at 1450.316 kb on + strand at 1450.480 kb on + strand, within MCAODC_23655 at 1450.541 kb on + strand, within MCAODC_23655 at 1450.542 kb on - strand, within MCAODC_23655 at 1450.634 kb on - strand at 1450.664 kb on - strand at 1450.723 kb on + strand at 1450.723 kb on + strand at 1450.727 kb on + strand at 1451.007 kb on + strand at 1451.652 kb on + strand, within opgE at 1451.960 kb on + strand, within opgE at 1452.536 kb on - strand at 1452.536 kb on - strand at 1452.608 kb on + strand at 1452.608 kb on + strand at 1452.860 kb on - strand, within dgkA at 1452.906 kb on - strand at 1452.909 kb on - strand at 1452.957 kb on - strand at 1453.199 kb on + strand at 1453.245 kb on - strand at 1453.310 kb on + strand at 1453.311 kb on - strand at 1453.311 kb on - strand at 1453.311 kb on - strand at 1453.376 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV219 remove 1,450,092 - -1.4 1,450,093 + -0.6 1,450,095 + +0.4 1,450,095 + +1.3 1,450,181 + +0.4 1,450,181 + -0.8 1,450,181 + -1.2 1,450,181 + -0.3 1,450,182 - -0.9 1,450,182 - -0.6 1,450,204 + +0.7 1,450,260 - +0.3 1,450,316 + -1.1 1,450,480 + MCAODC_23655 0.40 -0.1 1,450,541 + MCAODC_23655 0.62 +1.2 1,450,542 - MCAODC_23655 0.62 +0.7 1,450,634 - -1.2 1,450,664 - +2.0 1,450,723 + -0.3 1,450,723 + -2.2 1,450,727 + -0.7 1,451,007 + -1.3 1,451,652 + opgE MCAODC_23660 0.48 -2.0 1,451,960 + opgE MCAODC_23660 0.67 -0.1 1,452,536 - +1.7 1,452,536 - -0.3 1,452,608 + +0.5 1,452,608 + +1.6 1,452,860 - dgkA MCAODC_23665 0.77 +2.6 1,452,906 - -0.6 1,452,909 - -1.6 1,452,957 - -0.3 1,453,199 + -0.5 1,453,245 - +1.2 1,453,310 + +0.3 1,453,311 - +1.7 1,453,311 - +0.3 1,453,311 - +1.0 1,453,376 - +1.1
Or see this region's nucleotide sequence