Experiment: WM_EV219
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeeP and MCAODC_23320 are separated by 327 nucleotides MCAODC_23320 and MCAODC_23325 are separated by 213 nucleotides MCAODC_23325 and MCAODC_23330 overlap by 4 nucleotides MCAODC_23330 and MCAODC_23335 are separated by 53 nucleotides MCAODC_23335 and MCAODC_23340 overlap by 119 nucleotides
MCAODC_23315: yeeP - 50S ribosome-binding GTPase, at 1,396,701 to 1,397,573
yeeP
MCAODC_23320: MCAODC_23320 - hypothetical protein, at 1,397,901 to 1,398,083
_23320
MCAODC_23325: MCAODC_23325 - IS66 transposase, at 1,398,297 to 1,398,386
_23325
MCAODC_23330: MCAODC_23330 - putative transposase of insertion sequence IS682, at 1,398,383 to 1,398,808
_23330
MCAODC_23335: MCAODC_23335 - Integrase catalytic domain-containing protein, at 1,398,862 to 1,399,110
_23335
MCAODC_23340: MCAODC_23340 - BfpM-like protein, at 1,398,992 to 1,399,492
_23340
Position (kb)
1398
1399 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1397.450 kb on + strand, within yeeP at 1397.573 kb on + strand at 1397.573 kb on + strand at 1397.573 kb on + strand at 1397.573 kb on + strand at 1397.573 kb on + strand at 1397.573 kb on + strand at 1397.573 kb on + strand at 1397.573 kb on + strand at 1397.574 kb on - strand at 1397.574 kb on - strand at 1397.579 kb on + strand at 1397.579 kb on + strand at 1397.580 kb on - strand at 1397.580 kb on - strand at 1397.674 kb on - strand at 1397.690 kb on - strand at 1397.690 kb on - strand at 1397.690 kb on - strand at 1397.713 kb on - strand at 1397.906 kb on + strand at 1397.939 kb on - strand, within MCAODC_23320 at 1398.303 kb on + strand at 1398.303 kb on + strand at 1398.304 kb on - strand at 1398.304 kb on - strand at 1398.326 kb on + strand, within MCAODC_23325 at 1398.566 kb on + strand, within MCAODC_23330 at 1398.578 kb on + strand, within MCAODC_23330 at 1398.578 kb on + strand, within MCAODC_23330 at 1398.630 kb on - strand, within MCAODC_23330 at 1398.630 kb on - strand, within MCAODC_23330 at 1398.801 kb on - strand at 1398.812 kb on + strand at 1398.812 kb on + strand at 1398.812 kb on + strand at 1398.812 kb on + strand at 1398.812 kb on + strand at 1398.813 kb on - strand at 1398.824 kb on + strand at 1398.826 kb on + strand at 1398.842 kb on - strand at 1398.872 kb on - strand at 1399.189 kb on + strand, within MCAODC_23340
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV219 remove 1,397,450 + yeeP MCAODC_23315 0.86 +0.5 1,397,573 + +0.4 1,397,573 + +1.0 1,397,573 + -0.0 1,397,573 + -0.3 1,397,573 + +0.1 1,397,573 + +0.2 1,397,573 + +0.1 1,397,573 + +0.1 1,397,574 - -0.4 1,397,574 - +0.2 1,397,579 + -0.7 1,397,579 + +1.5 1,397,580 - -0.8 1,397,580 - -0.5 1,397,674 - +0.0 1,397,690 - +1.0 1,397,690 - +0.4 1,397,690 - +0.5 1,397,713 - +2.3 1,397,906 + +0.2 1,397,939 - MCAODC_23320 0.21 -2.1 1,398,303 + +0.5 1,398,303 + -0.2 1,398,304 - -0.0 1,398,304 - +1.3 1,398,326 + MCAODC_23325 0.32 -1.0 1,398,566 + MCAODC_23330 0.43 +1.4 1,398,578 + MCAODC_23330 0.46 -2.9 1,398,578 + MCAODC_23330 0.46 +0.4 1,398,630 - MCAODC_23330 0.58 -0.9 1,398,630 - MCAODC_23330 0.58 +0.7 1,398,801 - -1.8 1,398,812 + -0.6 1,398,812 + +0.8 1,398,812 + -2.9 1,398,812 + +0.3 1,398,812 + -0.4 1,398,813 - -1.4 1,398,824 + -2.4 1,398,826 + +1.5 1,398,842 - +0.5 1,398,872 - -2.5 1,399,189 + MCAODC_23340 0.39 -0.5
Or see this region's nucleotide sequence