Experiment: WM_EV219
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_17230 and MCAODC_17235 overlap by 4 nucleotides MCAODC_17235 and MCAODC_17240 are separated by 105 nucleotides MCAODC_17240 and iS285 are separated by 49 nucleotides
MCAODC_17230: MCAODC_17230 - Exonuclease, at 333,149 to 334,393
_17230
MCAODC_17235: MCAODC_17235 - site-specific integrase, at 334,390 to 334,926
_17235
MCAODC_17240: MCAODC_17240 - DinI-like protein Z2083/ECs2153, at 335,032 to 335,346
_17240
MCAODC_17245: iS285 - IS256 family transposase, at 335,396 to 336,265
iS285
Position (kb) 334
335 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 333.430 kb on + strand, within MCAODC_17230 at 333.431 kb on - strand, within MCAODC_17230 at 333.431 kb on - strand, within MCAODC_17230 at 333.438 kb on + strand, within MCAODC_17230 at 333.438 kb on + strand, within MCAODC_17230 at 333.442 kb on + strand, within MCAODC_17230 at 333.490 kb on + strand, within MCAODC_17230 at 333.532 kb on + strand, within MCAODC_17230 at 333.637 kb on - strand, within MCAODC_17230 at 333.640 kb on + strand, within MCAODC_17230 at 333.640 kb on + strand, within MCAODC_17230 at 333.643 kb on - strand, within MCAODC_17230 at 333.643 kb on - strand, within MCAODC_17230 at 333.775 kb on + strand, within MCAODC_17230 at 333.816 kb on - strand, within MCAODC_17230 at 333.816 kb on - strand, within MCAODC_17230 at 334.211 kb on - strand, within MCAODC_17230 at 334.211 kb on - strand, within MCAODC_17230 at 334.279 kb on + strand at 334.279 kb on + strand at 334.280 kb on - strand at 334.280 kb on - strand at 334.328 kb on - strand at 334.328 kb on - strand at 334.426 kb on - strand at 334.426 kb on - strand at 334.729 kb on + strand, within MCAODC_17235 at 334.746 kb on - strand, within MCAODC_17235 at 334.857 kb on - strand, within MCAODC_17235 at 335.607 kb on + strand, within iS285 at 335.764 kb on - strand, within iS285 at 335.836 kb on + strand, within iS285 at 335.836 kb on + strand, within iS285 at 335.836 kb on + strand, within iS285 at 335.837 kb on - strand, within iS285 at 335.872 kb on - strand, within iS285
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV219 remove 333,430 + MCAODC_17230 0.23 +0.8 333,431 - MCAODC_17230 0.23 +2.5 333,431 - MCAODC_17230 0.23 -0.1 333,438 + MCAODC_17230 0.23 -0.3 333,438 + MCAODC_17230 0.23 +1.0 333,442 + MCAODC_17230 0.24 -1.2 333,490 + MCAODC_17230 0.27 -0.7 333,532 + MCAODC_17230 0.31 +0.1 333,637 - MCAODC_17230 0.39 -0.0 333,640 + MCAODC_17230 0.39 -0.2 333,640 + MCAODC_17230 0.39 -0.4 333,643 - MCAODC_17230 0.40 +1.3 333,643 - MCAODC_17230 0.40 +0.3 333,775 + MCAODC_17230 0.50 -0.2 333,816 - MCAODC_17230 0.54 +0.4 333,816 - MCAODC_17230 0.54 +0.2 334,211 - MCAODC_17230 0.85 -0.7 334,211 - MCAODC_17230 0.85 +1.2 334,279 + +1.0 334,279 + +0.6 334,280 - -0.2 334,280 - -0.4 334,328 - -1.4 334,328 - +0.8 334,426 - +2.3 334,426 - +0.5 334,729 + MCAODC_17235 0.63 -0.9 334,746 - MCAODC_17235 0.66 +0.0 334,857 - MCAODC_17235 0.87 -2.8 335,607 + iS285 MCAODC_17245 0.24 -0.5 335,764 - iS285 MCAODC_17245 0.42 -0.4 335,836 + iS285 MCAODC_17245 0.51 +0.5 335,836 + iS285 MCAODC_17245 0.51 +0.9 335,836 + iS285 MCAODC_17245 0.51 -0.4 335,837 - iS285 MCAODC_17245 0.51 -1.8 335,872 - iS285 MCAODC_17245 0.55 +0.0
Or see this region's nucleotide sequence