Experiment: WM_EV219
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycjG and MCAODC_15980 overlap by 26 nucleotides MCAODC_15980 and wcaG are separated by 290 nucleotides wcaG and MCAODC_15990 are separated by 20 nucleotides
MCAODC_15975: ycjG - L-Ala-D/L-Glu epimerase, at 86,732 to 87,697
ycjG
MCAODC_15980: MCAODC_15980 - Murein peptide amidase A, at 87,672 to 88,400
_15980
MCAODC_15985: wcaG - NAD-dependent dehydratase, at 88,691 to 89,329
wcaG
MCAODC_15990: MCAODC_15990 - Hydrolase, at 89,350 to 89,973
_15990
Position (kb) 87
88
89 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 86.748 kb on + strand at 86.769 kb on + strand at 86.770 kb on - strand at 86.770 kb on - strand at 86.845 kb on + strand, within ycjG at 86.848 kb on + strand, within ycjG at 86.848 kb on + strand, within ycjG at 86.917 kb on + strand, within ycjG at 86.938 kb on - strand, within ycjG at 86.995 kb on + strand, within ycjG at 87.070 kb on - strand, within ycjG at 87.089 kb on - strand, within ycjG at 87.306 kb on + strand, within ycjG at 87.319 kb on - strand, within ycjG at 87.453 kb on - strand, within ycjG at 87.453 kb on - strand, within ycjG at 87.463 kb on + strand, within ycjG at 87.469 kb on + strand, within ycjG at 87.648 kb on + strand at 87.696 kb on - strand at 87.967 kb on - strand, within MCAODC_15980 at 87.967 kb on - strand, within MCAODC_15980 at 87.967 kb on - strand, within MCAODC_15980 at 87.968 kb on + strand, within MCAODC_15980 at 88.024 kb on - strand, within MCAODC_15980 at 88.026 kb on + strand, within MCAODC_15980 at 88.026 kb on + strand, within MCAODC_15980 at 88.124 kb on + strand, within MCAODC_15980 at 88.426 kb on - strand at 88.561 kb on + strand at 88.565 kb on + strand at 88.566 kb on - strand at 88.603 kb on + strand at 88.635 kb on - strand at 88.660 kb on - strand at 88.739 kb on + strand at 88.783 kb on + strand, within wcaG at 88.804 kb on + strand, within wcaG at 88.805 kb on - strand, within wcaG at 88.811 kb on + strand, within wcaG at 88.847 kb on - strand, within wcaG at 88.855 kb on - strand, within wcaG at 89.011 kb on - strand, within wcaG at 89.044 kb on + strand, within wcaG at 89.089 kb on + strand, within wcaG at 89.118 kb on + strand, within wcaG at 89.126 kb on - strand, within wcaG at 89.183 kb on - strand, within wcaG at 89.183 kb on - strand, within wcaG at 89.194 kb on + strand, within wcaG at 89.238 kb on - strand, within wcaG at 89.238 kb on - strand, within wcaG
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV219 remove 86,748 + -0.7 86,769 + -0.2 86,770 - -1.1 86,770 - +0.7 86,845 + ycjG MCAODC_15975 0.12 +0.9 86,848 + ycjG MCAODC_15975 0.12 +0.3 86,848 + ycjG MCAODC_15975 0.12 +0.5 86,917 + ycjG MCAODC_15975 0.19 -1.0 86,938 - ycjG MCAODC_15975 0.21 +0.4 86,995 + ycjG MCAODC_15975 0.27 +1.4 87,070 - ycjG MCAODC_15975 0.35 -2.9 87,089 - ycjG MCAODC_15975 0.37 +0.4 87,306 + ycjG MCAODC_15975 0.59 -0.0 87,319 - ycjG MCAODC_15975 0.61 +0.1 87,453 - ycjG MCAODC_15975 0.75 -0.2 87,453 - ycjG MCAODC_15975 0.75 -0.2 87,463 + ycjG MCAODC_15975 0.76 -0.7 87,469 + ycjG MCAODC_15975 0.76 -0.1 87,648 + -0.9 87,696 - -0.5 87,967 - MCAODC_15980 0.40 -0.3 87,967 - MCAODC_15980 0.40 -2.1 87,967 - MCAODC_15980 0.40 -0.7 87,968 + MCAODC_15980 0.41 -2.8 88,024 - MCAODC_15980 0.48 -2.8 88,026 + MCAODC_15980 0.49 -0.6 88,026 + MCAODC_15980 0.49 -0.1 88,124 + MCAODC_15980 0.62 +0.7 88,426 - +1.1 88,561 + -0.0 88,565 + +0.7 88,566 - +0.5 88,603 + +1.9 88,635 - -0.0 88,660 - -0.6 88,739 + -0.8 88,783 + wcaG MCAODC_15985 0.14 +0.0 88,804 + wcaG MCAODC_15985 0.18 +1.3 88,805 - wcaG MCAODC_15985 0.18 -0.6 88,811 + wcaG MCAODC_15985 0.19 +0.1 88,847 - wcaG MCAODC_15985 0.24 +1.0 88,855 - wcaG MCAODC_15985 0.26 -0.3 89,011 - wcaG MCAODC_15985 0.50 -0.1 89,044 + wcaG MCAODC_15985 0.55 -0.5 89,089 + wcaG MCAODC_15985 0.62 -2.4 89,118 + wcaG MCAODC_15985 0.67 +0.6 89,126 - wcaG MCAODC_15985 0.68 -0.1 89,183 - wcaG MCAODC_15985 0.77 -0.4 89,183 - wcaG MCAODC_15985 0.77 -0.1 89,194 + wcaG MCAODC_15985 0.79 -0.1 89,238 - wcaG MCAODC_15985 0.86 -0.3 89,238 - wcaG MCAODC_15985 0.86 +0.3
Or see this region's nucleotide sequence